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A method for directly extracting plant urease from soil to prepare calcium carbonate

A technology of calcium carbonate and plant enzymes, applied in the direction of fermentation, etc., can solve the problems of manpower and material resources, consumption, and affect the effect of solidification, and achieve the effect of high extraction efficiency and easy operation

Active Publication Date: 2022-04-22
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Chinese patent CN201610828703.2 discloses a method of using microorganisms to prepare calcium carbonate to solidify sand. The sand is poured into the bottle so that it evenly covers the bottom of the bottle, and then the gelling liquid and the cultured bacteria liquid are poured in, and the culture is shaken at a constant temperature. , and then quantitatively draw part of the mixed solution in the bottle every day, then add the same amount of gelling solution, and evenly add a small amount of sand, and finally obtain a solidified sand column after several days; but the cultivation of bacteria in this method often consumes a lot of manpower Material resources, and the cultivation process needs to strictly control the temperature and pH value of the growth of the bacteria, which is difficult to meet in the actual engineering site, which directly affects the curing effect

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  • A method for directly extracting plant urease from soil to prepare calcium carbonate
  • A method for directly extracting plant urease from soil to prepare calcium carbonate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for directly extracting plant urease from soil to prepare calcium carbonate, the steps are as follows:

[0019] 1) Collect local soil samples in Nanjing (in the small garden of Sipailou Small Campus of Southeast University) with an organic matter content of 20-40 g / kg, take soil samples within a depth of 5-30cm, put the soil samples into a sterilized sample bag and seal them Store in a refrigerator at 4°C.

[0020] 2) Extract and isolate plant urease from soil samples:

[0021] a) Weigh 200g of the soil sample, pour it into 200ml of 32% acetone and ethanol mixture, shake it sufficiently to make the soil sample a suspension, and let it stand at room temperature for 4 hours;

[0022] b) Pour the filtered soil supernatant into a beaker filled with 200ml of 32% acetone and ethanol mixture, oscillate to make it evenly mixed, then let it stand at room temperature for 4 hours, then filter twice, and finally Obtain an enzyme solution.

[0023] 3) Dilute 1 mL of the ...

Embodiment 2

[0027] The difference from Example 1 is that in step (4), the molar concentration of the calcium acetate solution is 0.75 mol / L, and the yield of calcium carbonate precipitation induced by microorganisms is 57%.

Embodiment 3

[0029] The difference from Example 1 is that in step (4), the molar concentration of the calcium acetate solution is 1 mol / L, and the microbial-induced calcium carbonate precipitation yield is 49%.

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Abstract

The invention relates to a method for directly extracting plant urease from soil to prepare calcium carbonate. The soil sample with an organic matter content of 20-40 g / kg is collected on site, and a mixture of acetone and pure ethanol is used as an extraction solvent to separate plant urease from the soil. , add urea after dilution, urea is hydrolyzed to CO 3 2‑ and NH 4 1+ , maintained at a constant temperature for more than 5 days, added calcium acetate solution, and sprayed the obtained mixture on a plate covered with sand, the Ca in the solution 2+ with hydrolyzed CO 3 2‑ Combined to form calcium carbonate precipitate. The operation is simple, no complicated bacterial cultivation and reproduction are required, and plant urease is directly used to catalyze the necessary reactions, thereby inducing carbonate precipitation, and the extraction efficiency of calcium carbonate is high, so it has low environmental requirements and is easy to popularize and apply.

Description

technical field [0001] The invention belongs to organically induced calcium carbonate solidification, which can be used for sand prevention and sand consolidation, and environment improvement. Background technique [0002] Biodiagenetic and mineralization is a natural phenomenon, which has played an important role in the history of the formation of the earth. This is the result of nearly 30 years of geochemical research. Re-understanding the role and status of biodiagenetic and mineralization has great impact on geotechnical engineering. Technological development is of great significance. The principle is that urease is produced during the growth and reproduction of microorganisms, which continuously decomposes urea to form CO 3 2- , in the negatively charged aqueous solution at the bacterial cell membrane interface, it continuously chelates Ca in the environment with organic matter 2+ , inducing a local crystal anion (CO 3 2- ), with CO 3 2- The concentration is furt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00
Inventor 缪林昌孙潇昊
Owner SOUTHEAST UNIV