Genital tract flora nucleic acid preservation solution formula and preparation and application method

A preservation solution and reproductive tract technology, applied in the field of microorganisms, can solve the problems of reproductive tract flora composition changes, nucleic acid degradation, cell wall rupture, etc., and achieve the effects of facilitating downstream gene detection, protecting microbial diversity, and facilitating collection and transportation
CN109735600AInactive Publication Date: 2019-05-10深圳市金锐生物科技有限公司
6 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
深圳市金锐生物科技有限公司
Publication Date
2019-05-10
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a genital tract flora nucleic acid preservation solution formula. A genital tract flora nucleic acid preservation solution comprises a Tris-HCl buffer solution with the concentration of 0.01-1 M, ethylenediaminetetraacetic acid with the concentration of 0.01-1 M, sodium chloride with the concentration of 0.01-0.5 M, sodium citrate with the concentration of 0.01-0.5 M and 10-30% anhydrous ethanol. The pH value of the prepared preservation solution is 8.0-10.0. The preservation solution is prepared through the method and used for collecting a genital tract sample, has the advantages of protecting microorganisms and nucleic acids thereof in the genital tract from being degraded, protecting the microbial diversity, facilitating collection and transportation of the genital tract sample and facilitating downstream gene detection, is beneficial to genital tract flora diversity research, and is applied to gene detection by taking genital tract floras as target points.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of microbes, and more specifically, to a formula, preparation and use method of a genital tract flora nucleic acid preservation solution. Background technique

[0002] Bacteria inhabit almost every part of the human body, and the number is in the trillions, up to 1014, which is about 10 times the total number of human cells. It is roughly estimated that there are nearly a thousand species of bacteria in the human gastrointestinal tract. Compared with the intestinal flora, the types of vaginal flora are simpler. There can be about 40 genera and more than 100 species of bacteria on the side wall mucosa around the vagina of women of childbearing age, weighing up to 20 grams. Including Lactobacillus, Atopobium vaginalis, Macrococcus, Corynebacterium, Escherichia coli, Veillonella and Gardnerella, etc. These vaginal bacteria can be roughly divided into two categories: "beneficial bacteria" and "conditional path...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More