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Quasi-wild habitat orchid mycorrhizal fungus and cymbidium longibracteatum seed symbiosis culture method

A technology of mycorrhizal fungi and symbiotic culture, applied in the field of plant biology, can solve the problems of inability to grow limited, low survival rate, etc., and achieve the effect of a simple operation path

Inactive Publication Date: 2019-05-17
SOUTHWEAT UNIV OF SCI & TECH
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Problems solved by technology

At present, the germination of Orchidaceae seeds mostly uses artificial medium for non-symbiotic germination under sterile conditions. Although the germination rate is high, when returning to the field, the survival rate of seedlings transplanted to the natural environment is low, and because they cannot be compared with Fungi in nature establish symbiotic relationships that result in severely restricted subsequent growth

Method used

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  • Quasi-wild habitat orchid mycorrhizal fungus and cymbidium longibracteatum seed symbiosis culture method
  • Quasi-wild habitat orchid mycorrhizal fungus and cymbidium longibracteatum seed symbiosis culture method
  • Quasi-wild habitat orchid mycorrhizal fungus and cymbidium longibracteatum seed symbiosis culture method

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Embodiment 1

[0025] Preparation of spring sword capsules: adopt the method of reciprocal cross breeding, the hybrid combination form is 'Yuhaitang' (♀)×'Huangmei' (♂), when the female parent flower is just opened, use sterile tweezers to remove the anther, and carry out after 3 days of flowering For artificial pollination, use sterile tweezers to remove the pollen mass of the male parent, move the pollen mass to the stigma at the top of the female parent's stamen, and let the mucus secreted by the stamen cavity stick the pollen mass, and pollinate 2- 3 flowers, after pollination, put them in a sterile gauze bag, place a tag to indicate the time of pollination, and manage them in isolation. One month after pollination, cut off the weak capsules, keep 1-2 strong capsules in each pot, and apply 1000 times of potassium dihydrogen phosphate solution 2-3 times when the capsules start to expand, so as to promote seed development. About 180 days after pollination, the fruit pods are green and ripe...

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Abstract

The invention discloses a quasi-wild habitat orchid mycorrhizal fungus and cymbidium longibracteatum seed symbiosis culture method. Endophytic orchidaceous rhizoctonia separated from orchids mycorrhiza and cymbidium longibracteatum rhizome tissue homogenate are mixed to serve as an inoculant, mature seeds in cymbidium longibracteatum capsules are sowed on the inoculant to germinate, and a fungus plant symbiotic system is established in the cymbidium longibracteatum native wild environment to obtain cymbidium longibracteatum seedlings. The method particularly comprises the following steps: cultivating orchidaceous rhizoctonia, preparing the inoculant, disinfecting the surfaces of the capsules, establishing a symbiotic system, performing management after inoculation, performing microscopic examination on a fungus plant system, performing mycorrhiza examination, performing seedling regression and the like. According to the technology of the invention, sexual mass propagation of the cymbidium longibracteatum can be realized, and important theory and application value in cymbidium longibracteatum breeding and continuous use of cymbidium longibracteatum resources are achieved.

Description

technical field [0001] The invention belongs to the field of plant biology. Specifically, it relates to a method of using Orchidaceous rhizoctonia (Orchidaceous rhizoctonia) and a homogenate of a spring sword tissue to make an inoculant and a spring sword seed to simulate a wild habitat for symbiotic culture to obtain a spring sword seedling. Background technique [0002] Chunjian is a plant of the genus Orchidaceae. It is a very important plant resource with high ecological and economic value. The "Huangmei" and "Jade Begonia" unearthed in wild resources have been exhibited at the Lambeau Expo many times. Received a gold medal. For a long time, due to blind and predatory excavation, wild spring sword resources are on the verge of extinction. However, the seeds of Spring Sword are very small and have no endosperm. Under natural conditions, seed germination needs to rely on specific symbiotic fungi to obtain nutrients, and the probability of seedlings germinating from its s...

Claims

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Application Information

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IPC IPC(8): A01G22/60A01G18/10A01C1/00A01C1/08
Inventor 李杰匡萍张巧
Owner SOUTHWEAT UNIV OF SCI & TECH
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