Method for increasing potassium content of tobacco leaves by combining microbial bacterial fertilizer with microbial fermentation liquid

A technology of microbial bacterial fertilizer and biological bacterial fertilizer, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of improving absorption capacity, improving soil fertility, and increasing soil organic matter

Inactive Publication Date: 2019-05-17
云南省农业科学院生物技术与种质资源研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the deficiencies in existing technique...

Method used

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  • Method for increasing potassium content of tobacco leaves by combining microbial bacterial fertilizer with microbial fermentation liquid
  • Method for increasing potassium content of tobacco leaves by combining microbial bacterial fertilizer with microbial fermentation liquid
  • Method for increasing potassium content of tobacco leaves by combining microbial bacterial fertilizer with microbial fermentation liquid

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Experimental program
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Embodiment 1

[0027] Shafu Bacillus (Bacillus safensis) colony figure in the present invention has the following biological characteristics: on the LB solid medium, the colony is smaller, milky yellow, with smooth and delicate edges, and a diameter of 0.3-1.1mm, see figure 1 , figure 2 . Tequila bacillus (Bacillus tequilensis) bacterium colony figure among the present invention has following biological characteristics: on LB solid medium, bacterium colony is pink, flat, and edge is rough, and diameter is 0.6-1.6mm, see image 3 , Figure 4 .

[0028] 1. Preparation of fermentation broth

[0029] Bacillus safensis (Bacillus safensis) and Bacillus tequilensis (Bacillus tequilensis) preserved in solid were fermented and cultured respectively by using different improved spore-producing liquid medium to obtain Bacillus safensis fermentation broth and Bacillus tequilensis fermentation broth Bacterial liquid, mix above-mentioned two kinds of bacterial liquids. The number of effective viable ...

Embodiment 2

[0040] 1. Preparation of fermentation broth

[0041] Bacillus safensis (Bacillus safensis) and Bacillus tequilensis (Bacillus tequilensis) preserved in solid were fermented and cultured respectively by using different improved spore-producing liquid medium to obtain Bacillus safensis fermentation broth and Bacillus tequilensis fermentation broth Bacterial liquid, mix above-mentioned two kinds of bacterial liquids. The number of effective viable bacteria in the fermentation broth is more than or equal to 100 million / g (cfu). The composition of the improved spore-producing liquid medium adopted by the spore bacillus is as follows: glucose 2.5g, yeast extract 1.5g, peptone 1g, KH 2 PO 4 0.3g, NaCl 2.0g, (NH 4 ) 2 SO 4 1.0g, Na 2 B 4 o 7 10H 2 O 4.5mg, anhydrous CaCl 2 0.33g, Na 2 MoO 4 2H 2 O0.03mg, H 2 O 1000m1. The improved spore-producing liquid medium composition that described Bacillus tequila adopts is as follows: glucose 2.0g, peptone 1.0g, NaCl 1.5g, (NH 4...

Embodiment 3

[0052] 1. Preparation of fermentation broth

[0053] Bacillus safensis (Bacillus safensis) and Bacillus tequilensis (Bacillus tequilensis) preserved in solid were fermented and cultured respectively by using different improved spore-producing liquid medium to obtain Bacillus safensis fermentation broth and Bacillus tequilensis fermentation broth Bacterial liquid, mix above-mentioned two kinds of bacterial liquids. The number of effective viable bacteria in the fermentation broth is more than or equal to 100 million / g (cfu). The composition of the improved spore-producing liquid medium adopted by the spore bacillus is as follows: glucose 2.5g, yeast extract 1.5g, peptone 1g, KH 2 PO 4 0.3g, NaCl 2.0g, (NH 4 ) 2 SO 4 1.0g, Na 2 B 4 o 7 10H 2 O 4.5mg, anhydrous CaCl 2 0.33g, Na 2 MoO 4 2H 2 O0.03mg, H 2 O 1000m1. The improved spore-producing liquid medium composition that described Bacillus tequila adopts is as follows: glucose 2.0g, peptone 1.0g, NaCl 1.5g, (NH 4...

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Abstract

The invention discloses a method for increasing potassium content of tobacco leaves by combining microbial bacterial fertilizer with microbial fermentation liquid. The method comprises the following steps: (1) preparing the microbial fermentation liquid; (2) preparing the bacterial fertilizer; (3) applying base fertilizer; (4) watering with the microbial fermentation liquid. The method utilizes bacillus safensis and bacillus tequilensis to prepare the microbial fermentation liquid and the bacterial fertilizer, and adopts a way of combining the microbial fermentation liquid and the bacterial fertilizer; the bacillus safensis and the bacillus tequilensis are applied respectively in solid state form and a liquid state form, and are promoted to colonize in the soil so as to finally form a dominant bacterial community; the bacterial fertilizer and the microbial fermentation liquid are organically combined together, so that the potassium content of the tobacco leaves can be effectively increased. The method reduces the consumption of chemical fertilizer and chemical pesticide, protects and promotes the growth of tobaccos, and promotes environmental friendliness.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to using bacterial fertilizers produced by Bacillus safensis and Bacillus tequilensis in combination with their fermented liquors to increase the potassium content of tobacco leaves. Background technique [0002] Potassium is a crucial indicator for evaluating the quality of tobacco leaves. The lower the potassium content of tobacco leaves, the worse their combustibility, and the tar content of single-material smoke may be high, which is not conducive to the development of high-end cigarettes. The higher the value, the stronger the combustibility, the more complete the tobacco leaves will be burned, the tar content will be reduced correspondingly, and the commodity value of the tobacco leaves will be improved. The potassium content of high-quality flue-cured tobacco leaves in the United States is 4% to 6%, and some even reach 8% to 10%, while the potassium content ...

Claims

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Application Information

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IPC IPC(8): C05F17/00C05G3/04C12N1/20C12R1/07C05G3/80
CPCY02W30/40
Inventor 周晓罡张滨姚春馨余萍丁玉梅杨映平
Owner 云南省农业科学院生物技术与种质资源研究所
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