Phlegm resolving liquid for extracting free DNAs from cell-free supernatant of sputum specimen

A cell-free and sputum technology, applied in the field of sputum removal, can solve the problems of low cfDNA content in cell-free supernatant, affecting the accuracy of gene detection results, etc., and achieve the effects of abundant cfDNA content, easy availability, and widespread promotion.
CN109762808AActive Publication Date: 2019-05-17BEIJING HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING HOSPITAL
Publication Date
2019-05-17

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Abstract

The invention provides a phlegm resolving liquid for extracting free DNAs from a cell-free supernatant of a sputum specimen. The phlegm resolving liquid consists of physiological saline and a dithiothreitol water solution with the concentration of 0.1-0.7 mol / L; the physiological saline and the dithiothreitol water solution are independently packaged and stored before use; normal saline is mixed with the sputum specimen and the dithiothreitol water solution on the use site. The volume ratio of the physiological saline to the dithiothreitol water solution is 9:1. Reagents of a formula are easily obtained, a preparation method is simple, and wide popularization is promoted. Compared with a phlegm resolving liquid reagent which has been applied clinically, the phlegm resolving liquid has advantages in the aspects of the phlegm resolving effect, the amount of obtained cfDNAs and the accuracy of a gene change detection result. Since the amount of the obtained cfDNAs is large, different kinds of genes can be detected. The formula belongs to isotonic liquids; after the cell-free supernatant is collected through short-term treatment, a cell fixing liquid is added into a precipitate, correspondingly cell morphology cannot be damaged, and a subsequent cytological pathological diagnosis result is not influenced.
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Description

technical field

[0001] The present invention relates to a phlegm-resolving liquid for extracting free DNA in the cell-free supernatant of a sputum sample, and in particular to a phlegm-resolving liquid formula for extracting non-small cell lung cancer (non-small cell lung cancer, NSCLC) patients The sputum sample has no free DNA (cell free DNA, cfDNA) in the supernatant, and the obtained cfDNA can be used for detection of relevant driver gene changes in NSCLC patients. Background technique

[0002] In recent years, the role of targeted drugs in the clinical tumor treatment of NSCLC has been confirmed, which is the so-called "precision treatment". The application of targeted drug therapy requires different gene change detection to select patients, and at the same time, corresponding drug selection is carried out for different gene changes carried. After tumor progression in patients resistant to targeted drug therapy, genetic alteration detection is still required to guide s...

Claims

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