Method for rapidly detecting Sudan red in pepper products

A detection method, Sudan red technology, applied in the detection field of Sudan red, can solve the problems of complicated operation and high price, and achieve the effect of accurate quantitative results, reduced time and good extraction effect

Inactive Publication Date: 2019-05-17
GUANGXI UNIVERSITY OF TECHNOLOGY +1
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Problems solved by technology

[0003] At present, high performance liquid chromatography, gas chromatography-mass spectrometry and liquid chromatography-mas

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  • Method for rapidly detecting Sudan red in pepper products
  • Method for rapidly detecting Sudan red in pepper products
  • Method for rapidly detecting Sudan red in pepper products

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content taught in the present invention, those skilled in the art can make various modifications or changes to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0041] 1. Experimental part

[0042] 1.1 Determination of sample pretreatment method

[0043] Accurately weigh 0.0010 g of Sudan Red I, II, III, and IV standard products, dissolve them into 40.00 mL of blank chili oil, and ultrasonicate for 10 min. After fully dissolving, a spiked sample of 25 μg / mL is obtained.

[0044] The added chili oil is evenly divided into two parts, one part is processed according to the national stand...

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Abstract

The invention relates to a Sudan red detection method and in particular to a method for rapidly detecting Sudan red in pepper products. According to the method, a pepper product is rapidly extracted by using acetone and acetonitrile so as to obtain extracting solution S, a liquid chromatography-ultraviolet visible fiber optic spectrometer is applied to analyzing the extracting solution S, and dataprocessing is carried out to obtain a background spectral database N which does not contain Sudan red; ultraviolet-visible spectral databases V of a series of Sudan red standard products with concentrations; and finally, a vector-subspace included angle criterion method is used for analyzing Sudan red in the pepper product: the to-be-detected product is rapidly extracted to obtain extracting solution C and ultraviolet-visible spectral data of the extracting solution C is acquired to calculate the content of Sudan red in the to-be-detected pepper product. The method is rapid and good in effectand is capable of respectively carrying out qualitative recognition and quantitative analysis on pure spectrums of Sudan red I, II, III and IV in mixed spectrums of marked samples.

Description

technical field [0001] The invention relates to a detection method for Sudan red, in particular to a rapid detection method for Sudan red in pepper products. Background technique [0002] In order to pursue a broader food market, some merchants add some food additives to their products to obtain food with beautiful colors and good appearance, to please consumers and to obtain good returns. Sudan red is also illegally added to food. Sudan red dye is carcinogenic, and its metabolites in the human body are all toxic organic compounds. If it is ingested for a long time, it will accumulate in the body and cause damage to the human body or gene mutation and cause cancer. Strengthening the detection and supervision of Sudan Red, an illegal additive in food, is of great significance to maintaining food safety and protecting human health. [0003] At present, the detection of Sudan red mainly adopts high performance liquid chromatography, gas chromatography-mass spectrometry and li...

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Application Information

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IPC IPC(8): G01N30/88
Inventor 刘柳徐传杰韩莹忻欣林华姚志湘廖小梅刘金梦
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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