Competitive ELISA (enzyme-linked immunosorbent assay) detection kit for type-O foot-and-mouth disease virus antibody

Abstract

The invention discloses a detection kit prepared from type-O foot-and-mouth disease VLPs (virus-like particles) and a preparation method of the detection kit. The preparation method of the detection kit includes that HRP is directly marked on a competitive antibody in simultaneous action with a tested antibody, internal ELISA plate coating type-O foot-and-mouth disease virus-like particles are formed by assembly of structural proteins VP0 and VP1 and optimized VP3 of the type-O foot-and-mouth disease virus. The adopted VLPs are available for efficient assembly without affecting diagnosis efficiency, and cost of diagnosis preparations can be reduced. Compared with the prior art, the method has advantages of reduction of detection steps, simplicity in testing operation, low workload, short time and improvement of specificity and sensitivity of the diagnostic reagents.

Description

technical field [0001] The invention relates to a virus detection kit and a preparation method thereof. Specifically, the invention relates to a detection kit and a preparation method prepared by using O-type foot-and-mouth disease virus-like particles (VLPs). Background technique [0002] Foot-and-mouth disease (FMD) is a highly contagious disease caused by foot-and-mouth disease virus (FMDV) that often occurs in cloven-hoofed animals and is zoonotic. The host range of FMDV is very wide, and there are more than 70 kinds of susceptible animals. The disease can be transmitted through the air and other ways, and it spreads rapidly with a 100% incidence rate, which can cause a worldwide pandemic. The onset symptoms are mainly manifested in blisters, salivation, body temperature height, or ulcers or scabs after damage to the hooves, mouth, and breasts. As a result, FMD causes a substantial drop in livestock productivity. The disease causes huge economic losses in the world ev...

AI Technical Summary

Problems solved by technology

Most of them use inactivated virus, recombinant virus, single protein or polypeptide as antigen, which leads to high false positive, low specificity, poor sensitivity, low accuracy and heavy workload in the existing ELISA antibody detection technology Or various shortcomings such as higher experimental conditions

And there is a safety risk in using the complete virion as an antigen, and the immunogenicity of a single protein or polypeptide is relatively poor

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