Wheat germ protein hydrolysate and preparation method and application thereof

A technology of wheat germ protein and hydrolyzate, which is applied in the field of wheat germ protein derivatives, can solve the problems of increased drug resistance, high toxicity and side effects of Helicobacter pylori infection, etc., and achieves a mild method, less drug-resistant bacteria, and low preparation cost Effect

Active Publication Date: 2019-05-31
QIQIHAR UNIVERSITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The present invention provides a wheat germ protein hydrolyzate in order to overcome the problems of large toxic and side effects and increasing drug resistance year by year in the treatment of Helicobacter pylori infection by existing antibiotic therapy A preparation method, the wheat germ protein hydrolyzate prepared by this method has significant activity against Helicobacter pylori adhering to gastric mucosal cells, and has the advantages of low cost and good safety at the same time

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  • Wheat germ protein hydrolysate and preparation method and application thereof
  • Wheat germ protein hydrolysate and preparation method and application thereof
  • Wheat germ protein hydrolysate and preparation method and application thereof

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preparation example Construction

[0039] The invention provides a kind of preparation method of wheat germ protein hydrolyzate, comprises the following steps:

[0040] (1) Extracting the wheat germ powder with an alkaline solution with a pH value of 9 to 10, separating the solid from the liquid, and obtaining the supernatant as an alkaline extract;

[0041] (2) adjusting the pH value of the alkali extract to 3.5 to 4.5, leaving it to stand, separating the solid from the liquid, and the resulting precipitate is wheat germ protein;

[0042] (3) enzymolyzing the wheat germ protein with protease for 2.5 to 5 hours, separating solid and liquid, drying the obtained supernatant, and obtaining a hydrolyzate of wheat germ protein;

[0043] The protease includes Pronase or Protease N.

[0044] The invention uses wheat germ powder as raw material to prepare wheat germ protein hydrolyzate. The wheat germ powder is derived from commercially available products, and degreased wheat germ powder can also be selected as the pr...

Embodiment 1

[0068] The preparation of embodiment 1 pronase wheat germ protein hydrolyzate

[0069] Wheat germ protein was extracted from defatted wheat germ powder by alkali extraction and acid precipitation. Weigh 100g of defatted wheat germ powder, add 1L of deionized water (w / v=1:10), adjust the pH to 9.5 with 1M sodium hydroxide while stirring at 400rpm, and centrifuge at 4000rpm for 10min after stirring for 30min. Collect the supernatant. The resulting precipitate was treated once by repeating the above steps, and the two supernatants were combined, and the pH of the solution was adjusted to 4.0 with 1M hydrochloric acid while stirring at 400 rpm, stirred for 30 minutes, left to stand for 20 minutes, centrifuged at 4000 rpm for 10 minutes, and the precipitate was collected. The precipitate was washed three times with 200 mL of deionized water at pH 4.0, dispersed with 50 mL of deionized water, and the pH was adjusted to 7.0, and the material obtained after freeze-drying was wheat ge...

Embodiment 2

[0071] Example 2 Detection of Anti-H.pylori Adhesion Activity of Pronase Protease Wheat Germ Protein Hydrolyzate

[0072] Experiment preparation:

[0073] (1) Culture of Helicobacter pylori

[0074] Since H.pylori ( 43504 TM ) is a model strain, so the present invention selects this strain as an anti-adhesion activity experimental strain.

[0075] The frozen H. pylori strain was thawed at 37°C and first suspended in #18 liquid medium to obtain a Helicobacter pylori mixture. The mixture was then inoculated onto #260 slant medium supplemented with 5% defibrinated sheep blood, in a microaerobic environment (5% O 2 , 10% CO 2 , 85%N 2 ) at 37° C. for 48 to 72 hours to obtain a Helicobacter pylori bacterial liquid (H. pylori bacterial liquid), which can be used for anti-adhesion activity detection and strain subculture.

[0076] The preparation method of #18 liquid medium is as follows: Weigh 17 grams of tryptone, 3 grams of soybean peptone, 5 grams of sodium chloride and 2...

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Abstract

The invention provides a wheat germ protein hydrolysate and a preparation method and application thereof, and relates to the technical field of wheat germ protein derivatives. The preparation method of the wheat germ protein hydrolysate adopts wheat germ powder as a raw material to carry out alkali extraction and acid precipitation so as to obtain wheat germ protein, and then the obtained wheat germ protein is enzymatically decomposed by pronase or protease N so as to obtain the wheat germ protein hydrolysate. Tests show that the wheat germ protein hydrolysate has the remarkable activity of resisting adhesion of helicobacter pylori to gastric mucosal cells, and can be used for preventing helicobacter pylori infection. Compared with existing medicine, the medicine has the advantages of being natural, safe, low in price, high in efficiency, mild, not prone to production of generate drug-resistant bacteria and the like. A new utilization way for wheat processing by-products, and the additional value of wheat processing is increased.

Description

technical field [0001] The invention relates to the technical field of wheat germ protein derivatives, in particular to a wheat germ protein hydrolyzate and its preparation method and application. Background technique [0002] 1. Overview of deep processing and comprehensive utilization of wheat germ in China [0003] Wheat is the food crop with the largest sowing area, the largest output, and the widest distribution in the world. At the same time, China is the largest country in wheat production, with an annual output of more than 100 million tons. However, in the process of processing wheat into flour, the existence of wheat germ will affect the processing quality, baking quality and storage period of flour. Therefore, wheat germ is one of the main by-products of wheat processing industry. The annual output of wheat germ in China is about 3 million tons. For a long time, due to the limitations of development technology and other issues, China's wheat germ resources have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12A23J3/18A23J3/34A23L33/18
Inventor 孙晓宏张凇源任健刘晓兰
Owner QIQIHAR UNIVERSITY
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