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Adulteration detection method to fibrates lipid-lowering chemicals of tea by high-performance thin-layer chromatograph(HPTLC)-bioluminescent method

A high-efficiency thin-layer chromatography and bioluminescence technology, applied in the field of food testing, can solve problems such as background interference and lack of selectivity for multiple targets

Active Publication Date: 2019-05-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this model is simple and efficient, it is not suitable for the detection of illegal addition of Chinese and Western medicines in tea, because it has the following two serious defects: heavy background noise
Lack of selectivity for multiple targets

Method used

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  • Adulteration detection method to fibrates lipid-lowering chemicals of tea by high-performance thin-layer chromatograph(HPTLC)-bioluminescent method
  • Adulteration detection method to fibrates lipid-lowering chemicals of tea by high-performance thin-layer chromatograph(HPTLC)-bioluminescent method
  • Adulteration detection method to fibrates lipid-lowering chemicals of tea by high-performance thin-layer chromatograph(HPTLC)-bioluminescent method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Preparation of simulated seawater liquid and solid medium: Prepare simulated seawater liquid medium according to the following formula: 30 g / L NaCl, 5 g / L Na 2 HPO 4 , 5 g / L KH 2 PO 4 , 3 ml / L glycerin, 5 g / L peptone, and 5 g / L yeast extract. After adding 1 L of ultrapure water and stirring to dissolve, adjust the pH value of the prepared liquid medium to 7.5±0.2 with 1 mol / L sodium hydroxide aqueous solution, and then use a high-pressure steam sterilizer to sterilize at 121°C for 15 minutes. The prepared liquid culture medium is packaged and stored in the refrigerator for later use, and can be stored at 4°C for 7 days when not in use.

[0041] (2) Cultivation and preservation of luminescent bacteria strains: inoculate the luminescent bacteria frozen and preserved in glycerol into the Erlenmeyer flask containing 100 mL of liquid medium prepared in step (1). The mouth of the bottle is wrapped with sterilized four-layer folded tinfoil to ensure that external oxyge...

Embodiment 2

[0052] (1) Preparation of simulated seawater liquid and solid medium: Prepare simulated seawater liquid medium according to the following formula: 30 g / L NaCl, 5 g / L Na 2 HPO 4 , 5 g / L KH 2 PO 4 , 3 ml / L glycerin, 5 g / L peptone, and 5 g / L yeast extract. After adding 1 L of ultrapure water and stirring to dissolve, adjust the pH value of the prepared liquid medium to 7.5±0.2 with 1 mol / L sodium hydroxide aqueous solution, and then use a high-pressure steam sterilizer to sterilize at 121°C for 15 minutes. The prepared liquid culture medium is packaged and stored in the refrigerator for later use, and can be stored at 4°C for 7 days when not in use.

[0053] (2) Cultivation and preservation of luminescent bacteria strains: inoculate the luminescent bacteria frozen and preserved in glycerol into the Erlenmeyer flask containing 100 mL of liquid medium prepared in step (1). The mouth of the bottle is wrapped with sterilized four-layer folded tinfoil to ensure that external oxyge...

Embodiment 3

[0065] (1) Preparation of simulated seawater liquid and solid medium: prepare simulated seawater liquid medium according to the following formula: 30 g / L NaCl, 5 g / L Na 2 HPO 4 , 5 g / L KH 2 PO 4 , 3 ml / L glycerin, 5 g / L peptone, and 5 g / L yeast extract. After adding 1 L of ultrapure water and stirring to dissolve, adjust the pH value of the prepared liquid medium to 7.5±0.2 with 1 mol / L sodium hydroxide aqueous solution, and then use a high-pressure steam sterilizer to sterilize at 121°C for 15 minutes. The prepared liquid culture medium is packaged and stored in the refrigerator for later use, and can be stored at 4°C for 7 days when not in use.

[0066] (2) Cultivation and preservation of luminescent bacteria strains: inoculate the luminescent bacteria frozen and preserved in glycerol into the Erlenmeyer flask containing 100 mL of liquid medium prepared in step (1). The mouth of the bottle is wrapped with sterilized four-layer folded tinfoil to ensure that external oxyge...

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Abstract

The invention discloses an adulteration detection method to fibrates lipid-lowering chemicals of tea by the high-performance thin-layer chromatograph(HPTLC)-bioluminescent method and belongs to the technical field of food detection. The adulteration detection method includes, firstly, preparing standard product solution of bezafibrate and ciprofibrate and a tea sample; secondly, pre-washing thin-layer plates and performing HPTLC sample application; thirdly, performing HPTLC separation to move target objects mixed originally to different positions of the thin-layer plates according to difference of molecular structures so as to form physical isolation; finally, simultaneously detecting multiple targets of the samples conveniently through luminous strains coupled with the thin-layer plate byan impregnation method. The adulteration detection method refers to a method capable of detecting lipid-lowering chemicals of tea rapidly quantitatively by the HPTCL-bioluminescent method, and has the advantages of being economic, rapid, simple and convenient.

Description

technical field [0001] The invention relates to a method for screening the adulteration of fibrates lipid-lowering chemical drugs in tea leaves by high-efficiency thin-layer chromatography combined with bioluminescence, and specifically relates to a method for organically integrating HPTLC high-throughput separation with bioluminescence inhibition imaging detection. The invention relates to a high-throughput screening method for adulterating illegal chemicals in different tea products, belonging to the technical field of food testing. Background technique [0002] The "rich man's disease" caused by unscientific daily diet has replaced pathogenic microbial infection as the most important threat to human health. Among them, hyperlipidemia is one of the most common "diseases of wealth". Hyperlipidemia not only directly has a serious negative impact on human health, but also easily leads to complications such as coronary heart disease, hypertension, cerebral thrombosis and ather...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/95
CPCG01N33/14G01N30/94G01N2030/945A23L33/105A23F3/00A23L33/10G01N30/95G01N33/92G01N2570/00
Inventor 陈益胜舒蓝萍王了徐学明张煌黄彩虹金征宇谢正军柏玉香
Owner JIANGNAN UNIV
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