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Medicine and functional food for preventing and treating post-traumatic stress syndrome

A functional food, post-traumatic stress technology, applied in the field of preparation of amino acid oral liquid, can solve the problem of no research report on the prevention and treatment of mental diseases and so on

Active Publication Date: 2019-06-07
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that it has various pharmacological effects such as anti-tumor, immune regulation and regulation of Drosophila exercise ability imbalance, but there is no research report on its effect on the prevention and treatment of mental diseases at home and abroad

Method used

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  • Medicine and functional food for preventing and treating post-traumatic stress syndrome
  • Medicine and functional food for preventing and treating post-traumatic stress syndrome
  • Medicine and functional food for preventing and treating post-traumatic stress syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 SMLC enhances the antioxidant capacity of brain tissue

[0025] The luminol reaction was used to detect the total antioxidant capacity of brain tissue. We use the brain tissue-hydrogen peroxide-luminol luminescence system to observe the chemiluminescence produced by the reaction of hydrogen peroxide and luminol under the catalysis of some enzymes in the brain tissue. Hydrogen peroxide was purchased from Merck. Drugs such as luminol, SMLC and Trolox were purchased from Sigma Company with a purity of >99%. The detection instrument is a Bayer Centaur 240 automatic chemiluminescence instrument, 0.1mol / L PBS 135μL, 5mmol / L luminol 15μL, rat hippocampus tissue extract supernatant 10μL, and 20μL of different concentrations of SMLC were added to the measurement tube successively. Test solution (5mM, 1mM) or positive control strong antioxidant Trolox (62.5μM), mix well, put in the instrument at 37℃, add 20μL of hydrogen peroxide (100mM) to start the reaction, after a...

Embodiment 2

[0026] Example 2 SMLC reduces the accumulation of ROS in nerve cells

[0027] The fluorescent probe DCFH-DA was used to detect ROS in nerve cell line SH-SY5Y. DCFH-DA itself has no fluorescence and can freely pass through the cell membrane. After entering the cell, it can be hydrolyzed by intracellular lipase to generate DCFH. However, DCFH cannot permeate the cell membrane, so the probe can be easily loaded into the cell. Intracellular ROS can oxidize non-fluorescent DCFH to produce fluorescent DCF. The fluorescence of DCF represents the level of reactive oxygen species in the cells. The human neuroblastoma cell line SH-SY5Y was cultured in DMEM medium containing 15% fetal bovine serum in a 5% CO2 incubator at 37 degrees Celsius. Passage every 3-5 days. Seed SH-SY5Y into 6-well plates before the experiment. Nerve cell line SH-SY5Y treated with different drug concentrations of SMLC (0mM, 0.5mM, 1mM), cultured for 24 hours, added hydrogen peroxide 250μM for 1 hour, removed...

Embodiment 3

[0028] Example 3 SMLC reduces nerve cell damage caused by hydrogen peroxide

[0029] The human neuroblastoma cell line SH-SY5Y was cultured in DMEM medium containing 15% fetal bovine serum in a 5% CO2 incubator at 37 degrees Celsius. Passage every 3-5 days. Seed SH-SY5Y into a 96-well plate before the experiment. 250 μM hydrogen peroxide was used for 12 hours to create a cell model of oxidative stress injury. SMLC (1 mM and 0.5 mM) was added to the medium 2 hours before the addition of hydrogen peroxide and maintained until the end of the experiment. After the experiment, the cell viability was detected by MTT. Aspirate the culture supernatant in each well, add 200ul of serum-free cell culture medium containing 0.5mg / ml MTT and continue to incubate for 4 hours, terminate the culture, aspirate the culture supernatant in the well, add 100μl DMSO to each well, shake for 10 After fully melting the crystals, perform colorimetry immediately, the measuring wavelength is 570nm, an...

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Abstract

The invention discloses medicine or functional food for preventing and / or treating post-traumatic stress syndrome. The medicine or functional food consists of S-methyl-L-cysteine(thiomethy L-cysteine)or amino acid salts of thiomethy L-cysteine and additives or / and carriers acceptable in pharmacy or food industry. The S-methyl-L-cysteine can be provided by a plant extract which is used as a raw material and is rich in the S-methyl-L-cysteine. The medicine or functional food can generate a dissipation promoting effect on fear memory related to traumatic stress by reducing oxidative stress levelin a body, reducing free radicals and enhancing synaptic plasticity and other characteristics dependent on NMDA receptors in relevant brain regions, thereby treating behavioral abnormalities caused by post-traumatic stress.

Description

technical field [0001] The invention relates to a preparation method of an amino acid oral liquid and its new application in the preparation of medicines and functional foods for preventing and treating post-traumatic stress syndrome. Background technique [0002] With the development of our society and the acceleration of the pace of life, the accompanying pressure and stress have become the source of people's troubles. Moderate stress is good for the body to develop tolerance and resistance to adapt to stressors, but sudden severe and unpredictable stress can cause many people to suffer from mental illness. Posttraumatic stress disorder (PTSD) refers to a delayed and persistent mental disorder caused by individuals experiencing, witnessing or encountering one or more traumatic events. Its core symptoms include: Traumatic re-experiencing Symptoms, avoidance and numbness-like symptoms, and symptoms of increased alertness seriously impair the patient's physical and mental he...

Claims

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Application Information

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IPC IPC(8): A61K31/198A61K9/08A61P39/00A61P39/06A61P25/00
Inventor 陈建国吴鹏飞王芳关鑫磊沈祖成罗涵
Owner HUAZHONG UNIV OF SCI & TECH
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