Preparation method of allograft-derived platelet-rich gelator

Abstract

The invention discloses a preparation method of an allograft-derived platelet-rich gelator. The method comprises the steps as follows: (1) umbilical cord blood collection; (2) primary centrifugation:centrifuging a blood sample at the centrifugation parameter of 1500 r / min for 10 min; sucking a supernatant, a middle layer and the upper end of a red blood cell layer for 0.5-1 mm after centrifugation, and performing secondary centrifugation; (3) secondary centrifugation: performing secondary centrifugation at the centrifugation parameter of 2800 r / min for 10 min; sucking a supernatant liquid after centrifugation until 5 ml of the lower layer is left, which is the platelet-rich factor; and (4) uniformly mixing the platelet-rich factor with a calcium chloride-bovine thrombin solution in the volume ratio being 1:(8-10), leaving the mixture to stand for 2 min or longer to obtain the platelet-rich gelator. The allograft-derived platelet-rich gelator prepared by the method can be used for allograft therapy, has concentration up to 9 times that of original whole blood, can be used for acute and chronic wound repair and can be used as compound artificial bone for treatment of nonunion.

Description

technical field [0001] The invention relates to a preparation method of allogeneic platelet-rich gelatin factor, which belongs to the technical field of blood products. Background technique [0002] Platelet-rich factor is a platelet concentrate obtained by gradient centrifugation and separation of autologous whole blood, and is rich in platelets. The normal platelet concentration in human blood is 100,000 μl to 350,000 μl, with an average of 200,000 μl. The platelet concentration in platelet-rich factor is generally 3 to 10 times that of whole blood. When platelets are activated, they can release a variety of growth factors, which play an important role in promoting the proliferation, growth, differentiation and tissue formation of bone cells and osteoblasts. Since Marx et al first used platelet-rich factor composite bone graft to repair mandibular defects in 1998, platelet-rich factor has been gradually applied to tissue repair in the fields of stomatology, plastic surger...

AI Technical Summary

Problems solved by technology

[0010] In view of the above-mentioned prior art, in order to solve the limitation that the platelet-rich factor extracted and prepared in the prior art is only used for autologous treatment, and to solve the defect of the low concentration of the platelet-rich factor extracted and prepared in the prior art, the present invention provides a Preparation method of allogeneic platelet-rich gel factor