Method for preparing sn-1-DHA-hemolytic phosphatidylserine
A technology of phosphatidylserine and glycerophosphorylserine is applied in the field of preparing sn-1-DHA-lysophosphatidylserine, which can solve the problems of reducing the utilization efficiency of DHA and the transportation efficiency, and achieve the effect of high DHA purity
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Embodiment 1
[0040] A method for preparing sn-1-DHA-lysophosphatidylserine, comprising the following steps:
[0041] (1) Weigh 5.0g of soybean phosphatidylserine (wherein phosphatidylserine accounts for 35% of the total phospholipids) and dissolve it in 20mL of n-hexane, add 15g of absolute ethanol and 1.5g of immobilized Candida antarctica lipase Lipozyme 435 (provided by Novozymes), heated to 60 ° C and stirred for 2 h, then filtered the reaction mixture to reclaim the immobilized Candida antarctica enzyme, and the filtrate was removed at 60 ° C and -0.088 MPa to remove n-hexane solvent and unreacted ethanol. Then add 50mL of acetone to fully shake and wash and collect the acetone insoluble matter;
[0042] (2) Dissolve the acetone-insoluble material obtained in step (1) in 20 mL of chloroform after evaporating to dry acetone, add 1.0 g of absolute ethanol and 1.5 g of Lipozyme 435, stir and react at 60°C for 8 hours, then keep 60°C and apply Vacuum to remove chloroform solvent and unre...
Embodiment 2
[0045] (1) Weigh 5.0g of soybean phosphatidylserine (wherein phosphatidylserine accounts for 50% of the total phospholipids) and dissolve it in 20mL of n-hexane, add 5.0g of absolute ethanol and 0.05g of immobilized Candida antarctica lipase Lipozyme 435 and heat Stir the reaction at 30°C for 8 hours, then filter the reaction mixture to recover the immobilized enzyme, remove the n-hexane solvent and unreacted ethanol from the filtrate at 60°C and -0.088MPa, then add 50mL of acetone to fully shake and wash, and collect the acetone insoluble matter;
[0046] (2) Dissolve the acetone-insoluble material obtained in step (1) in 20 mL of chloroform after evaporating the acetone, add 0.5 g of absolute ethanol and 0.05 g of Lipozyme 435, stir and react at 30 ° C for 24 h, then heat to 60 ° C and add to the reaction Vacuum was applied to the system to remove chloroform solvent and unreacted ethanol, then 10 mL of deionized water was added and stirred thoroughly, then suction filtered, t...
Embodiment 3
[0049] (1) Weigh 5.0 g of soybean phosphatidylserine (wherein phosphatidylserine accounts for 80% of the total phospholipids) and dissolve it in 20 mL of n-hexane, add 10 g of absolute ethanol and 0.5 g of immobilized Candida antarctica lipase Lipozyme 435 and heat for 40 Stir the reaction at ℃ for 2 hours, then filter the reaction mixture to recover the immobilized enzyme, remove the n-hexane solvent and unreacted ethanol from the filtrate under the condition of 60 ℃ and -0.088MPa, then add 50mL acetone to fully shake and wash, and collect the acetone insoluble matter;
[0050] (2) Dissolve the acetone-insoluble material obtained in step (1) in 20 mL of chloroform after evaporating the acetone, add 0.5 g of absolute ethanol and 0.05 g of Lipozyme 435, stir and react at 30°C for 24 hours, then heat to 60°C and apply Vacuum to remove chloroform solvent and unreacted ethanol, then add 10mL deionized water and stir well, then filter with suction, then centrifuge the filtrate, disc...
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