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Method for screening prognostic markers of DNA methylation in acute myeloid leukemia

A prognostic marker, acute myeloid technology, applied in the field of biomedicine, can solve the problems of large heterogeneity, high morbidity and mortality of AML, defects in research methods, etc., to improve prognosis, improve the prognosis stratification system, The effect of good feasibility and practicality

Active Publication Date: 2019-06-07
深圳豪石生物科技有限公司
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Problems solved by technology

[0009] The technical problems to be solved by the present invention are: in view of the high morbidity and mortality of AML, the large heterogeneity, the current prognostic stratification system is still insufficient, DNA methylation is closely related to AML, and the previous methylation research methods have defects, MCC-Seq detection of methylation has multiple advantages. The present invention uses MCC-Seq technology to perform DNA methylation sequencing on AML bone marrow samples, quantitatively detects the methylation status of CpG sites in the whole genome of AML, and uses DNA methylation differences Analysis, looking for methylation regulatory genes related to poor prognosis, guiding the precise diagnosis and treatment of AML, improving the prognosis of patients, reducing the economic burden for the country and society, and contributing to the development of precision medicine

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  • Method for screening prognostic markers of DNA methylation in acute myeloid leukemia
  • Method for screening prognostic markers of DNA methylation in acute myeloid leukemia
  • Method for screening prognostic markers of DNA methylation in acute myeloid leukemia

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Embodiment Construction

[0043] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0044] Unless otherwise specified in the examples, the experimental methods used are conventional methods; the experimental materials, reagents, etc. used can be obtained through commercial channels.

[0045] 1. Experimental materials

[0046] 1. MCC-Seq sequencing case

[0047] The present invention selects AML patients, according to the inclusion criteria: (1) definitely diagnosed as non-M3 acute myeloid leukemia; (2) aged over 18 years; (3) at least one course of chemotherapy and evaluation of curative effect; (4) MICM diagnosis The data and clinical treatment data are complete; (5) There are mutation-targeted sequencing results (for accurate molecular biology prognostic stratification); (6) At least the bone marrow specimens at the time of initial treatment are preserved. ...

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Abstract

The invention discloses a method for screening prognostic markers of DNA methylation in acute myeloid leukemia. The method includes the following steps: (1) sample and clinical data collection; (2) sample preparation; (3) genomic DNA extration; (4) whole-genome CpG site methylation capture sequencing; and (5)methylation sequencing data analysis and marker screening. The method uses the whole-genome CpG site methylation capture sequencing technology to detect DNA methylation in acute myeloid leukemia and perform prognosis analysis, and screens genetic markers which are regulated by DNA methylation and has prognostic significance through information of integrative genetics and epigenetics to guide accurate diagnosis and treatment of AML, thereby improving the curative effect and improving the prognosis of patients.

Description

technical field [0001] The invention relates to a method for screening DNA methylation prognostic markers of acute myeloid leukemia, which belongs to the field of biomedicine. Background technique [0002] Leukemia is one of the most common malignant tumors in my country, and among children and young cancer patients under the age of 40, the death rate of leukemia ranks first. In 2016, Chinese scholars published an important article "Cancer Statistics in China, 2015", which revealed that the incidence of leukemia ranked 12th, and the mortality rate rose to 9th. There are about 50,000 new cases of leukemia every year, and the incidence rate and The death rate is showing an increasing trend year by year [1] . In leukemia, acute myeloid leukemia (acute myeloidleukemia, AML) is the main one, accounting for about 70% of the incidence of acute leukemia [2] . AML has complex pathogenesis, large clinical heterogeneity, and high recurrence and mortality [3-7] , treatment costs ar...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6811C12Q1/6886
Inventor 刘春蕙于川高丽军祁红双侯晨芳王雪阳
Owner 深圳豪石生物科技有限公司
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