A method for selecting and breeding Wuding chickens based on osteoprotegerin blood biochemical markers

A technology of osteoprotegerin, Wuding chicken, applied in the direction of instruments for radiological diagnosis, testing material strength by applying stable bending force, biological testing, etc.

Inactive Publication Date: 2019-06-07
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

There is no research on the effect of osteoprotegerin content on bone development of Wuding chickens. In order to meet people's needs for local chicken breeds, it is necessary to propose a new method for breeding Yunnan Wuding chickens with good bone development. The research on element and skeletal development, establish a blood biochemical marker, pave the way for the early breeding of Yunnan Wuding chicken

Method used

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  • A method for selecting and breeding Wuding chickens based on osteoprotegerin blood biochemical markers
  • A method for selecting and breeding Wuding chickens based on osteoprotegerin blood biochemical markers
  • A method for selecting and breeding Wuding chickens based on osteoprotegerin blood biochemical markers

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Collection and storage of experimental samples

[0022] (1) Preparation of serum

[0023] 20 Wuding chickens with normal skeletal development and abnormal skeletal development were randomly selected from the Wuding chicken family with normal skeletal development and Wuding chicken with abnormal skeletal development. Metabolism-related biochemical indicators. Water and food were cut off for 12 hours before blood collection, and blood was collected from the wing vein the next morning. Collect 3-4ml of blood sample in a 10ml centrifuge tube, let it stand for 30min, centrifuge at 3000rpm at room temperature for 20min, take the supernatant (serum) into a 1.5ml centrifuge tube, and store it in a -20°C refrigerator for later use.

[0024] (2) Collection of leg bone samples

[0025] 20 Wuding chickens with normal skeletal development and abnormal skeletal development were slaughtered at 1 and 2 months respectively, and the femurs and tibias of the left and right legs of t...

Embodiment 2

[0063] 100 one-month-old Wuding chickens with consistent body size and good health were selected in Wuding, Yunnan and its farms, and the OPG concentration values ​​in their blood were detected respectively. According to the obtained concentration values, they were statistically grouped within a certain range, and the following statistical tables were obtained. .

[0064] Table 9 The content statistics of OPG in one-month-old Wuding chicken

[0065]

[0066] These 5 groups of one-month-old Wuding chickens were raised to two-month-old under the same feeding conditions in strict accordance with the immunization program, and their tibial bone mineral density was measured respectively (using a portable bone densitometer to detect). The statistical results of each group are as follows (see table 10).

[0067] Table 10 Statistical statistics of average tibial bone density of one-month-old Wuding chickens

[0068]

[0069] It can be seen from Table 10 that the average tibial ...

Embodiment 3

[0081] 1. Preparation of experimental materials

[0082] A total of 120 one-month-old Wuding chickens (including normal and abnormal bone development types) and 120 two-month-old Wuding chickens (including normal and abnormal bone development types) were sampled in Wuding chicken farms in Wuding County, Yunnan Province. Skeletal dysplasia type), measure the bone density of the tibia respectively; take blood from the wing vein, collect the blood sample with a 5ml centrifuge tube, let it stand for 30 minutes, and centrifuge at 3000rpm for 20 minutes at room temperature, and take the supernatant, which is extremely serum.

[0083] 2. Detect the concentration of OPG in serum

[0084] Carry out strict specification operation according to OPG enzyme-linked immunoassay (ELISA) kit, apply double-antibody sandwich method to measure the level of chicken osteocalcin (OPG) in the sample, measure absorbance (OD value) at 450nm wavelength with microplate reader, pass The standard curve was...

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Abstract

The invention relates to a method for selecting and breeding Wuding chickens based on osteoprotegerin blood biochemical markers, and belongs to the technical field of poultry blood biochemical markers. The method comprises the following steps: 1) collecting blood samples of Wuding chickens to be detected, preparing serum and detecting osteoprotegerin (OPG) concentration thereof; and 2) comparing the concentration measured in the step 1) with the blood biochemical markers of the Wuding chickens to classify the Wuding chickens to be detected into the Wuding chickens with normal bone developmentand the Wuding chickens with abnormal bone development. By measuring change of concentration of osteoprotegerin in the serum of the Wuding chickens with normal bone development and the Wuding chickenswith abnormal bone development and combining the detected bone density and bone strength of the Wuding chickens, the method gives blood biochemical markers of the Wuding chickens with normal bone development and the Wuding chickens with abnormal bone development to screen out the high-quality chickens, thereby providing theoretical basis for early selection of the Wuding chickens, and providing anew idea for research on the breeding method of the Wuding chickens and breeding of Yunnan local chicken breeds.

Description

technical field [0001] The invention belongs to the technical field of poultry blood biochemical markers, and in particular relates to a method for breeding Wuding chickens based on osteoprotegerin blood biochemical markers. Background technique [0002] As an important organ in the poultry, the bone functions to support, protect and exercise the poultry's body. In the past century, the high-intensity production performance of poultry has had a negative impact on poultry bone development, resulting in increased bone development defects, osteoporosis, rickets and other bone diseases, affecting the healthy development of the poultry industry. Chicken bone density and strength are important economic traits of chickens. Every year, bone diseases such as bone development defects, osteoporosis, and rickets cause huge economic losses to the chicken industry. Studies in the United States have shown that the mortality rate caused by skeletal deformities is about 1.5%-3.2%, indicatin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N3/20G01N9/24G01N33/53G01N33/58A01K67/02A61B6/00
Inventor 豆腾飞闫世雄贾俊静李涛徐志强段小花谷大海刘丽仙葛长荣黄英李琦华荣华王坤孙帅张霞程志斌
Owner YUNNAN AGRICULTURAL UNIVERSITY
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