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Raistrickindole A and preparation method and anti-hepatitis c virus application thereof

A kind of technology of resindole and indole, which is applied in the field of medicine and biology

Active Publication Date: 2019-06-18
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Related to the chemical structure, preparation method and anti-hepatitis C virus application of the compound shown in formula I, so far, no relevant reports have been seen

Method used

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  • Raistrickindole A and preparation method and anti-hepatitis c virus application thereof
  • Raistrickindole A and preparation method and anti-hepatitis c virus application thereof
  • Raistrickindole A and preparation method and anti-hepatitis c virus application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Identification of the fungus IMB17-034 (Penicillium raistrickii)

[0049] a) Source of the strain: the fungus IMB17-034 (Penicillium raistrickii) was isolated from a sea mud sample (18°24′09.00″N, 109°51′08.00″E) collected by our laboratory from the Sanya mangrove forest.

[0050] b) Strain identification: The fungus IMB17-034 (Penicillium raistrickii) was identified based on the conservation of the DNA sequence of the ITS and 18S regions in the microbial species. The genome of the fungus IMB17-034 (Penicilliumraistrickii) was extracted, and its ITS and 18S rRNA genes were amplified by PCR and sequenced, and submitted to the NCBI GenBank database to obtain accession numbers MK027053 and MK204575. The sequences are as SEQ ID NO. 1 and SEQ ID NO. 2 shown.

[0051] SEQ ID NO.1

[0052]

[0053]

[0054] SEQ ID NO.2

[0055]

[0056] It was shown that the bacteria belonged to the genus Penicillium of the Aspergillus family. Accordingly, the bacteria was named Penicillium raistrick...

Embodiment 2

[0057] Fermentation of fungus IMB17-034 (Penicillium raistrickii)

[0058] Inoculate the spore liquid of the strain on a PDA medium plate [formulation: glucose 2.0g, potato extract powder 0.3g, agar powder 18g, deionized water 100mL], and place it in a 28℃ constant temperature incubator for 7-14 days. Sterile shovel to take about 1cm 2 The bacteria-containing agar block was inoculated into 100mL liquid PDB fermentation medium [the formula is: glucose 2.0g, potato extract 0.3g, deionized water 100mL], in a 500mL Erlenmeyer flask, 28℃, 200r / min, shake flask culture for 5d Make seed liquid. Next, transfer the seed liquid to a solid rice fermentation medium at a 10% inoculum amount [the formula is: 100g rice, 0.3g peptone, and artificial sea salt 3.0g deionized water 100mL], a total of 30 bottles, stand at 28°C. 30 days.

Embodiment 3

[0059] Extraction of fermentation products and obtainment of extract

[0060] The solid fermentation product of strain IMB17-034 was collected, and an equal volume of ethyl acetate was added for ultrasonic extraction 3 times, 60 minutes each time, and the solvent was recovered under reduced pressure to obtain an ethyl acetate extract.

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Abstract

The invention belongs to the field of medical biology and relates to a preparation method of a novel structural compound raistrickindole A acquired from marine-derived Penicillium raistrickii, and anti-HCV (anti-hepatitis c virus) application of this compound.

Description

Technical field: [0001] The present invention belongs to the field of medical biology, and relates to a preparation method of a new structural compound Raistrichindole A (English name: raistrickindole A) obtained from Penicillium lecidium from marine sources; the present invention also relates to the compound against hepatitis C virus (HCV) Infected application. Background technique: [0002] Hepatitis C virus (HCV) poses a serious threat to human health. It is estimated that there are approximately 170 million people chronically infected with HCV in the world [Lauer, et al. New England Journal of Medicine, 2001, 345, 41-51.]. More than 390,000 people die from HCV infection globally every year [World Health Organization, Global Hepatitis Report, 2017]. Patients with chronic hepatitis C have a high risk of developing fatal liver disease complications, including liver cirrhosis and liver cancer. Among them, the incidence of liver cirrhosis reaches 20%, and in patients with liver ...

Claims

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Application Information

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IPC IPC(8): C07D498/14A61K31/542A61P1/16A61P31/14C12P17/18C12N1/14C12R1/645
CPCC12P17/188C12N1/145C12R2001/80
Inventor 甘茂罗胡媛媛李娇陈明华郝晓萌肖春玲彭宗根
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI