Microsphere self-assembled laser based on coaxial three-waveguide fiber

Abstract

The invention provides a microsphere self-assembled laser system based on a coaxial three-waveguide fiber. The "fiber-microsphere" laser is mainly composed of the following four parts: (1) A coaxial three-waveguide optical fiber with a new structure. (2) microspherical optical resonant cavity, gain medium with optical amplification function in the cavity; (3) including light source and gain medium excitation light source that can provide microspheres to capture photodynamic force; (4) detection spectrometer for microsphere output laser. The output spectrum of the microsphere optical resonant cavity inside the cell is very sensitive to the slight changes of the environmental physical parameters such as the cytosol inside the cell, and can be amplified and measured by the laser output signal of the multi-core cone fiber. The invention can be used for single cell capture and cell laser spectrum measurement, and can be widely used in the technical fields of single cell manipulation, sensing, measurement and analysis.

Description

technical field [0001] The invention relates to a microsphere self-assembly laser based on a coaxial three-waveguide fiber, which can be used for microsphere capture, microsphere laser spectrum measurement and microsphere laser self-assembly, and is especially suitable for the technical fields of single cell manipulation, measurement and analysis . Background technique [0002] In 1960, American scientist T.H. Maiman and others successfully created the world's first ruby ​​crystal laser. In 1961, A. Jia Wen and others successfully developed a helium-neon laser. In 1962, R.N. Hall and others developed a gallium arsenide semiconductor laser. . The birth of lasers marks that people have the ability to control the emission direction, phase, frequency and polarization of multiple photons, which makes people's understanding and application of light reach a higher level. Lasers have shown unimaginable application value in the direction of miniaturization and interdisciplinary, so...

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Problems solved by technology

[0004] In June 2001, Gather et al. from Harvard University enabled human embryonic kidney cells to emit laser signals (NATUREPHOTONICS, Single-cell biological lasers, 2011, 5:406-410). The light spot is reduced to the size of a single cell, and a Fabry-Perot resonant cavity with a space slightly larger than the cell size is bonded by two high-reflection mirrors to limit the cells in the position of the excitation light, so the device is bulky. The direction and position of the spatial excitation light are inconvenient to adjust single cells, and the cells can only be captured by means of external space constraints.

In 2015, Humar et al. from Harvard Medical School developed a variety of cell lasers based on whispering gallery mode microcavities (NATURE PHOTONICS, Intracellular microlasers, 2015, 9:572-576), which proved that it can also be realized in natural cells For laser output, a regular circular lipid droplet is artificially embedded in the cell as a whispering gallery mode, and the output signal is coupled to the spectral detector through a multimode optical fiber with a core diameter of 200 μm, but the device is large in size, and the optical fiber used to receive the signal It is thicker and does not have micro-control functions such as precise capture of cells and regulation of the temperature around the cells, making the operation of irradiating the cells with the excitation beam less accurate, and the small displacement of the cells in the liquid will cause the excitation beam to be unable to be accurately coupled into the lipid. drop, so that the gain signal cannot be continuously enhanced, and it also increases the difficulty of the operation of the experiment

[0005] The invention patent with the patent number of CN201510295509.8 proposes a tunable liquid microsphere laser. In this patent, two optical fiber optical tweezers are required to capture the microspheres at the same time, and the output of one end of the optical fiber is opposite to the receiving mode of the other end of the optical fiber. Signal light; the invention patent with the patent number CN201510267391.8 proposes a droplet whispering gallery mode laser and its manufacturing method. In this patent, the input light needs to be coupled into the In the annular fiber core, the droplet also needs to be in contact with the micro-nano fiber to transmit the signal light; the invention patent with the patent number CN201510271055.0 proposes a multi-wavelength droplet laser. For excitation detection, the same as the previous patent, each drop needs to be in contact with a micro-nano fiber for output. This method undoubtedly increases the difficulty of the device. As we all know, the size of the micro-nano fiber is only a few microns, which is extremely vulnerable to The influence of the external environment, and it is difficult to keep the surface of the optical fiber clean for a long time, and the patent requires a linear arrangement of multiple liquid droplets, which means that a linear distribution of multiple micro-nano optical fibers is required. Due to the small size of the liquid droplets, This also places extremely high demands on the experimental operation.

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