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Method for improving property of microbial fuel cell sensor

A technology of fuel cells and microorganisms, applied in biochemical fuel cells, fuel cells, circuits, etc., can solve the problems that the sensitivity and stability of MFC sensors cannot be fundamentally improved, and the sensing performance declines, and achieve faster in-situ on-line monitoring process, promotes agility and strength, improves proportion and activity

Inactive Publication Date: 2019-06-21
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

[0006] For the above defects or improvement needs of the prior art, the invention provides a method for improving the performance of the microbial fuel cell sensor, by adding a quorum-sensing self-inducing agent to the culture solution of the microbial fuel cell sensor, and the MFC sensor is fully cycled. The regulation of the microbial community and activity in the electroactive biofilm can effectively solve the problem of the decline in sensing performance caused by the excessive proportion of dead cells in the anode biofilm, and significantly improve the sensitivity and environmental shock resistance of the MFC sensor, thus solving the existing The technical problem that the technology cannot fundamentally improve the sensitivity and stability of the MFC sensor

Method used

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  • Method for improving property of microbial fuel cell sensor

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Embodiment 1

[0047] (I) Construction of the MFC sensor: The MFC sensor is a 28mL cubic reactor, and the anode is a graphite plate with a size of 2cm×2cm×0.5cm. It is polished with sandpaper and soaked in a 1:3 concentrated sulfuric acid / hydrogen peroxide solution for 60s, and then Sonicate with pure water for 10 minutes, and fix the graphite plate with titanium wire after drilling holes. The cathode is selected by using polyvinylidene fluoride (PVDF) binder to mix and bond activated carbon and carbon black powder evenly on the stainless steel mesh, soak in pure water for 15 minutes, and air dry for later use. After fixing the anode and cathode in the reactor, connect the anode and cathode with titanium wire and alligator clips, and connect a 1000Ω resistor externally. The two ends of the external resistance are connected to the data acquisition instrument, and the voltage data at both ends of the external resistance are collected every 1 min.

[0048] figure 1 It is an MFC sensor constru...

Embodiment 2

[0053] Adopt same method to repeat embodiment 1, difference is, select 2mg / L-10mg / L Cu for use in described step (Ⅴ). 2+ solution for highly toxic heavy metal sensing experiments. After the MFC sensor replaces the culture medium, the output voltage rises and after the highest output voltage stabilizes for 2-3 hours, add a certain volume of Cu to the culture medium 2+ standard solution. Two hours after the sensing experiment, the MFC sensor culture medium was replaced with a fresh culture medium, and the recovery of the MFC output voltage signal was observed 4 hours after the culture medium was replaced.

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Abstract

The invention belongs to the field of microbial electrochemistry, and particularly relates to a method for improving the property of a microbial fuel cell sensor by quorum sensing from a microbial level. The method comprises the following steps that: in the whole operation process of a MFC (Microbial Fuel Cell) sensor, adding quorum sensing autoinducer into culture solution; and after the MFC sensor is stably started, applying the MFC sensor to water environment heavy metal sensing. A microbial quorum sensing phenomenon is used for regulating and controlling the electrogenesis bacteria ratio and the activity of an MFC sensor anode biological membrane. Compared with the prior art, the method disclosed by the invention can effectively solve the problem that the sensor property is lowered since an anode biological membrane dead cell ratio is overlarge, the method has the advantages of being simple in operation and stable in effects, the sensing flexibility and the toxic impact resistanceof the MFC sensor can be effectively improved, and a process that the MFC sensor realizes in-situ online monitoring is quickened.

Description

technical field [0001] The invention belongs to the field of microbial electrochemistry, and more specifically relates to a method for improving the performance of microbial fuel cell sensors from the microbial level by using quorum sensing. Background technique [0002] Due to the imperfect environmental supervision system and the substandard clean production technology, environmental pollution problems have occurred frequently in my country in recent years. All kinds of heavy metals and highly toxic organic and inorganic compounds are discharged into the natural environment through industrial wastewater and urban domestic wastewater, which seriously damages natural ecological functions and directly endangers public health and social stability. Therefore, there is an urgent need for an effective water toxicity early warning system. At present, the detection of toxic substances in the water environment mostly adopts non-situ chemical means, which has poor timeliness, high c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M8/04664H01M8/16G01N27/327G01N33/18
CPCY02E60/50
Inventor 刘冰川潘静怡卜晨鹏黎建峰汪东亮胡敬平侯慧杰杨家宽
Owner HUAZHONG UNIV OF SCI & TECH
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