Probiotics, fermenting agent and application in medicinal herb preparation

Abstract

The invention discloses probiotics, a fermenting agent and application in a medicinal herb preparation. The fermenting agent comprises 3 strains of probiotics, two kinds of bacillus have a complex enzyme producing effect, and the other kind of lactic acid bacteria has high stress resistance. According to the fermenting agent, Chinese herbal medicine cinnamomum camphora seeds and wingedtooth laggera herb can be effectively fermented, and liquid fermentation and solid fermentation are included. The inhibiting effect of the fermented Chinese herbal medicine on pathogenic vibrio can be remarkablyenhanced, and is 2-4 times that of the Chinese herbal medicine which is not fermented. The fermenting agent is applied to aquaculture, has a disease prevention and treatment effect, and is good in effect, free of drug resistance and environmentally friendly.

Description

technical field

[0001] The invention relates to a probiotic and a starter, in particular to an application of the probiotic, a starter and a Chinese herbal medicine preparation. Background technique

[0002] Dried fruit of camphor tree (Cinnamomum camphora), spherical, brown-black to purple-black, wrinkled and uneven on the surface, about 5-10 mm in diameter. Spicy and warm in nature, non-toxic, has the effects of expelling cold and dampness, promoting qi and relieving pain, inhibiting bacteria, treating vomiting and diarrhea, stomach cold abdominal pain, and swelling. Lingdancao is the dry aboveground part of Hexapteria pterygium. Harvest in autumn when the stems and leaves are lush. Its taste is pungent, bitter, cold in nature. It has the effects of clearing away heat and detoxification, relieving cough and eliminating phlegm.

[0003] Vibrio (Vibrio) is widely distributed in seawater and marine animals in estuaries, bays, and coastal waters. Vibriosis is a group of d...

Examples

preparation example Construction

[0030] 3. Preparation of starter

[0031] Bacillus subtilis BSX and Bacillus licheniformis BL3C were activated in LB liquid medium, transferred to 100mL LB liquid medium with 1% inoculum size, and cultivated for 14 hours; Enterococcus faecium Ef026 was activated in MRS liquid medium, with 1 The % inoculum size was transferred to 100mL MRS liquid medium, which was used as seed solution after 15 hours of cultivation. The well-growing Bacillus subtilis, Enterococcus faecium and Bacillus licheniformis in the logarithmic growth phase were mixed and fermented according to the bacterial activity ratio of 1:1:1.

[0032] 4. Preparation of Chinese herbal preparations

Embodiment 1

[0033] Embodiment 1, the preparation of liquid fermentation Cinnamomum camphora preparation

[0034] (1) Preparation of Chinese herbal medicine preparation from liquid fermented camphor seed

[0035] Cinnamomum camphora is crushed to 40 mesh, accounts for 15% of the total mass, water accounts for 70% of the total mass, inoculates the 15% starter prepared in the above 3, and ferments for 72 hours at 37°C.

[0036] Indicator bacteria: Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi etc.

[0037] (2) Antibacterial test of fermentation broth

[0038]The fermentation broth was sterilized by filtration through a 0.22nm membrane after sterilization, and diluted by the double dilution method. Take 100 μL of indicator bacteria and smear it on TCBS solid medium, spot 10 μL of fermentation broth and the double-diluted fermentation broth on the TCBS medium coated with indicator bacteria, cultivate overnight at 37°C, and observe the transparent circle.

[0039] The diamete...

Embodiment 2

[0040] Embodiment 2, the preparation of solid fermentation camphor seed preparation

[0041] (1) Preparation of Chinese herbal preparations by solid fermentation of Cinnamomum camphora.

[0042] Cinnamomum camphora is pulverized to 40 orders, accounts for 15% of total mass, bran accounts for 20% of total mass, corn flour accounts for 10% of total mass, soybean meal accounts for 9% of total mass, and ammonium sulfate accounts for 0.7% of total mass, Potassium dihydrogen phosphate accounts for 0.25% of the total mass, magnesium sulfate accounts for 0.05% of the total mass, water accounts for 30% of the total mass, inoculates 15% of the starter prepared in the above 3, and ferments for 72 hours at 30°C. The indicator bacteria are the same as shown in Example 1.

[0043] (2) Antibacterial test after fermentation

[0044] Take 1 g of the fermented Chinese herbal medicine preparation, fully dissolve it with 9 mL of sterile water, filter and sterilize it with a 0.22 nm membrane aft...

technical field

[0001] The invention relates to a probiotic and a starter, in particular to an application of the probiotic, a starter and a Chinese herbal medicine preparation. Background technique

[0002] Dried fruit of camphor tree (Cinnamomum camphora), spherical, brown-black to purple-black, wrinkled and uneven on the surface, about 5-10 mm in diameter. Spicy and warm in nature, non-toxic, has the effects of expelling cold and dampness, promoting qi and relieving pain, inhibiting bacteria, treating vomiting and diarrhea, stomach cold abdominal pain, and swelling. Lingdancao is the dry aboveground part of Hexapteria pterygium. Harvest in autumn when the stems and leaves are lush. Its taste is pungent, bitter, cold in nature. It has the effects of clearing away heat and detoxification, relieving cough and eliminating phlegm.

[0003] Vibrio (Vibrio) is widely distributed in seawater and marine animals in estuaries, bays, and coastal waters. Vibriosis is a group of d...

Examples

preparation example Construction

[0030] 3. Preparation of starter

[0031] Bacillus subtilis BSX and Bacillus licheniformis BL3C were activated in LB liquid medium, transferred to 100mL LB liquid medium with 1% inoculum size, and cultivated for 14 hours; Enterococcus faecium Ef026 was activated in MRS liquid medium, with 1 The % inoculum size was transferred to 100mL MRS liquid medium, which was used as seed solution after 15 hours of cultivation. The well-growing Bacillus subtilis, Enterococcus faecium and Bacillus licheniformis in the logarithmic growth phase were mixed and fermented according to the bacterial activity ratio of 1:1:1.

[0032] 4. Preparation of Chinese herbal preparations

Embodiment 1

[0033] Embodiment 1, the preparation of liquid fermentation Cinnamomum camphora preparation

[0034] (1) Preparation of Chinese herbal medicine preparation from liquid fermented camphor seed

[0035] Cinnamomum camphora is crushed to 40 mesh, accounts for 15% of the total mass, water accounts for 70% of the total mass, inoculates the 15% starter prepared in the above 3, and ferments for 72 hours at 37°C.

[0036] Indicator bacteria: Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi etc.

[0037] (2) Antibacterial test of fermentation broth

[0038]The fermentation broth was sterilized by filtration through a 0.22nm membrane after sterilization, and diluted by the double dilution method. Take 100 μL of indicator bacteria and smear it on TCBS solid medium, spot 10 μL of fermentation broth and the double-diluted fermentation broth on the TCBS medium coated with indicator bacteria, cultivate overnight at 37°C, and observe the transparent circle.

[0039] The diamete...

Embodiment 2

[0040] Embodiment 2, the preparation of solid fermentation camphor seed preparation

[0041] (1) Preparation of Chinese herbal preparations by solid fermentation of Cinnamomum camphora.

[0042] Cinnamomum camphora is pulverized to 40 orders, accounts for 15% of total mass, bran accounts for 20% of total mass, corn flour accounts for 10% of total mass, soybean meal accounts for 9% of total mass, and ammonium sulfate accounts for 0.7% of total mass, Potassium dihydrogen phosphate accounts for 0.25% of the total mass, magnesium sulfate accounts for 0.05% of the total mass, water accounts for 30% of the total mass, inoculates 15% of the starter prepared in the above 3, and ferments for 72 hours at 30°C. The indicator bacteria are the same as shown in Example 1.

[0043] (2) Antibacterial test after fermentation

[0044] Take 1 g of the fermented Chinese herbal medicine preparation, fully dissolve it with 9 mL of sterile water, filter and sterilize it with a 0.22 nm membrane aft...

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