Probiotics, fermenting agent and application in medicinal herb preparation
A fermentation agent and probiotic technology, applied in the field of probiotics and fermentation agents, can solve the problems of single type of enzyme production and weak enzyme production ability, and achieve the effects of improving nutrient absorption, improving inhibition, and reducing diarrhea rate
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[0030] 3. Preparation of starter
[0031] Bacillus subtilis BSX and Bacillus licheniformis BL3C were activated in LB liquid medium, transferred to 100mL LB liquid medium with 1% inoculum size, and cultivated for 14 hours; Enterococcus faecium Ef026 was activated in MRS liquid medium, with 1 The % inoculum size was transferred to 100mL MRS liquid medium, which was used as seed solution after 15 hours of cultivation. The well-growing Bacillus subtilis, Enterococcus faecium and Bacillus licheniformis in the logarithmic growth phase were mixed and fermented according to the bacterial activity ratio of 1:1:1.
[0032] 4. Preparation of Chinese herbal preparations
Embodiment 1
[0033] Embodiment 1, the preparation of liquid fermentation Cinnamomum camphora preparation
[0034] (1) Preparation of Chinese herbal medicine preparation from liquid fermented camphor seed
[0035] Cinnamomum camphora is crushed to 40 mesh, accounts for 15% of the total mass, water accounts for 70% of the total mass, inoculates the 15% starter prepared in the above 3, and ferments for 72 hours at 37°C.
[0036] Indicator bacteria: Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi etc.
[0037] (2) Antibacterial test of fermentation broth
[0038]The fermentation broth was sterilized by filtration through a 0.22nm membrane after sterilization, and diluted by the double dilution method. Take 100 μL of indicator bacteria and smear it on TCBS solid medium, spot 10 μL of fermentation broth and the double-diluted fermentation broth on the TCBS medium coated with indicator bacteria, cultivate overnight at 37°C, and observe the transparent circle.
[0039] The diamete...
Embodiment 2
[0040] Embodiment 2, the preparation of solid fermentation camphor seed preparation
[0041] (1) Preparation of Chinese herbal preparations by solid fermentation of Cinnamomum camphora.
[0042] Cinnamomum camphora is pulverized to 40 orders, accounts for 15% of total mass, bran accounts for 20% of total mass, corn flour accounts for 10% of total mass, soybean meal accounts for 9% of total mass, and ammonium sulfate accounts for 0.7% of total mass, Potassium dihydrogen phosphate accounts for 0.25% of the total mass, magnesium sulfate accounts for 0.05% of the total mass, water accounts for 30% of the total mass, inoculates 15% of the starter prepared in the above 3, and ferments for 72 hours at 30°C. The indicator bacteria are the same as shown in Example 1.
[0043] (2) Antibacterial test after fermentation
[0044] Take 1 g of the fermented Chinese herbal medicine preparation, fully dissolve it with 9 mL of sterile water, filter and sterilize it with a 0.22 nm membrane aft...
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