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Preparation method of phosphoric acid modified sugarcane marrow insoluble carrier for cellulose binding domain fusion enzyme immobilization

A technology of sugarcane pith and binding domain, applied in the field of enzyme engineering, can solve the problems of high cost and difficulty in meeting the needs of industrial production, and achieve the effect of solving the shortage

Active Publication Date: 2019-06-28
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing reports mainly use microcrystalline cellulose for modification to prepare insoluble immobilized carriers, which is expensive and difficult to meet the needs of existing industrial production
There is no report on the preparation of phosphoric acid-modified sugarcane pith insoluble carrier for immobilization of cellulose-binding domain fusion enzyme

Method used

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  • Preparation method of phosphoric acid modified sugarcane marrow insoluble carrier for cellulose binding domain fusion enzyme immobilization
  • Preparation method of phosphoric acid modified sugarcane marrow insoluble carrier for cellulose binding domain fusion enzyme immobilization
  • Preparation method of phosphoric acid modified sugarcane marrow insoluble carrier for cellulose binding domain fusion enzyme immobilization

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Effect test

Embodiment 1

[0028] A method for preparing a phosphoric acid-modified sugarcane pith insoluble carrier for immobilization of cellulose-binding domain fusion enzyme, comprising the following steps:

[0029] (1) the sugarcane pith is pulverized by a pulverizer, sieved by a 60-purpose sieve, and the sifted matter (fine powder) is collected, which is called sugarcane pith powder;

[0030] (2) Soak the sugarcane pith powder with water of 4 times the quality for 5 hours, filter out the water, and obtain wet sugarcane pith powder;

[0031] (3) Under ice-bath conditions, slowly add 40 times its mass of concentrated phosphoric acid that has been pre-frozen at -5 to -10°C to the wet sugarcane pith powder while stirring, and continue stirring intermittently for 2 hours under ice-bath until the mixture becomes transparent;

[0032] (4) adopting a stainless steel mesh screen greater than 80 meshes to filter out insolubles to obtain sugarcane pulp;

[0033] (5) Slowly add water under stirring at room ...

Embodiment 2

[0038] A method for preparing a phosphoric acid-modified sugarcane pith insoluble carrier for immobilization of cellulose-binding domain fusion enzyme, comprising the following steps:

[0039] (1) the sugarcane pith is pulverized by a pulverizer, sieved by an 80-purpose sieve, and the sieve (fine powder) is collected, which is called sugarcane pith powder;

[0040] (2) Soak the sugarcane pith powder with 5 times the quality of water for 8 hours, filter out the water, and obtain wet sugarcane pith powder;

[0041] (3) Under ice-bath conditions, slowly add concentrated phosphoric acid 50 times its mass that has been pre-frozen at -5 to -10°C to wet sugarcane pith powder while stirring, and continue stirring intermittently for 1 hour under ice-bath until the mixture becomes transparent;

[0042] (4) adopt 80 mesh stainless steel mesh screens to filter out insoluble matter, obtain sugarcane pulp;

[0043](5) Slowly add water under stirring at room temperature to dilute the sugar...

Embodiment 3

[0048] A method for preparing a phosphoric acid-modified sugarcane pith insoluble carrier for immobilization of cellulose-binding domain fusion enzyme, comprising the following steps:

[0049] (1) the sugarcane pith is pulverized by a pulverizer, sieved by a 60-purpose sieve, and the sifted matter (fine powder) is collected, which is called sugarcane pith powder;

[0050] (2) Soak the sugarcane pith powder with 6 times the quality of water for 6 hours, filter out the water, and obtain wet sugarcane pith powder;

[0051] (3) Under ice-bath conditions, slowly add 60 times the mass of concentrated phosphoric acid that has been pre-frozen at -5 to -10°C to the wet sugarcane pith powder while stirring, and continue to stir intermittently for 1.5 hours under ice-bath until the mixture become transparent;

[0052] (4) adopt 100 mesh stainless steel mesh screens to filter out insoluble matter, obtain sugarcane pulp;

[0053] (5) Slowly add water under stirring at room temperature to...

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Abstract

The invention relates to a preparation method of a phosphoric acid modified sugarcane marrow insoluble carrier for cellulose binding domain fusion enzyme immobilization. The method comprises the stepsof sugarcane marrow->crushing->sieving->sugarcane marrow powder->soaking with water->moisture filtering->concentrated phosphoric acid adding (volume percentage greater than 85%) ->stirring even and dispersing->ice bathing for 1-2h (intermittent mixing)->insoluble matter filtering->filter slurry collecting->ice water adding for diluting->stirring at room temperature for 0.5-1h->full precipitating->filtering or centrifuging->sediment collecting-> precipitate washing 3 times with water->precipitate re-suspending with water->suspension adjusting with a Na2CO3 or NaOH solution ->filtering or centrifuging->precipitate collecting-> precipitate washing 5 times with water->filtering or centrifuging->precipitate collecting-> phosphoric acid modified sugarcane marrow insoluble carrier; the method uses phosphoric acid to modify the sugarcane marrow, prepares the immobilized carrier capable of efficiently adsorbing the cellulose binding domain fusion enzyme, solves the problem of increasing the added value of the comprehensive utilization of the sugarcane marrow, and solves the problem of shortage of the immobilized carrier for enzyme engineering.

Description

technical field [0001] The invention relates to a preparation method of a phosphoric acid-modified sugarcane pith insoluble carrier used for immobilizing cellulose binding domain fusion enzyme, and belongs to the field of enzyme engineering. Background technique [0002] Bagasse is the main waste of the sugar industry (accounting for 24% to 27%). The total output of sugarcane in the southern sugarcane region is more than 70 million tons, and the annual bagasse output is about 20 million tons. In terms of comprehensive utilization of bagasse, the long bagasse part (mainly the cortex of sugarcane) is often used in papermaking, while the short fiber and fine powder part (mainly the core part of sugarcane, called sugarcane pith, Sugarcane pulp, SP) cannot be used as Papermaking raw materials are often put into boilers for combustion treatment, or degraded into xylooligosaccharides. The morphology of cells in sugarcane pith is nearly round, not fibrous (aspect ratio greater tha...

Claims

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Application Information

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IPC IPC(8): C12N11/12C08H8/00
Inventor 杨胜远韦锦
Owner LINGNAN NORMAL UNIV