CLIA (chemiluminescence immunoassay) device based on micro-fluidic chip and use method

Abstract

The invention provides a CLIA (chemiluminescence immunoassay) device based on a micro-fluidic chip and a use method and belongs to the field of biochemical inspection equipment. The device comprises areaction chamber, the micro-fluidic chip arranged in the reaction chamber and driven by a rotating motor to rotate, a lifting assembly, a magnetic ring, an acquisition module, a temperature control assembly and a control panel, wherein the lifting assembly is used for driving the rotating motor to move up and down to realize chip washing, reaction and switching of acquisition positions, the magnetic ring is arranged at the lower end of the inside of the reaction chamber and used for attracting magnetic beads to provide continuous magnetic attraction force during high-speed rotation of the chip, the acquisition module is used for acquiring optical signals and uploading the optical signals to the control panel, the temperature control assembly is used for controlling temperature of the reaction chamber to meet the enzyme reaction requirement, and the control panel controls the acquisition module, the temperature control assembly and the rotating motor to act and controls the micro-fluidic chip to rotate and stop. The CLIA device is high in integration degree, convenient to operate and capable of realizing full-automatic detection.

Description

Technical field [0001] The invention belongs to the field of biochemical inspection equipment, and relates to a chemiluminescence immunodetection device based on a microfluidic chip and a use method. Background technique [0002] Microfluidic chip technology is a new technology for precise manipulation and control of nanoliter and picoliter fluids (biological sample fluids) in micrometer-scale flow channels. The application of this technology can be used to prepare samples in the fields of chemistry and biology. The basic operation units of reaction, separation, detection, cell culture, sorting, lysis, etc. are integrated or basically integrated on a chip of a few square centimeters (or even smaller), and a network is formed by micro-channels to control the flow of fluid throughout the entire system. A technology platform to replace various functions of conventional chemical or biological laboratories. The basic feature and biggest advantage of the microfluidic lab-on-a-chip is ...

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Problems solved by technology

[0008] At present, there is no related equipment on the market based on microfluidic chips and multi-item simultaneous detection. After the production of double-antibody sandwich complexes, in the process of washing and removing unbound enzyme-labeled antibodies, the method adopted by traditional large-scale detection equipment is to absorb multiple times -Dilution-absorption method, the process is complicated and produces more waste liquid

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