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Thin layer quantitative image recognition detection method for water-soluble natural product

A natural product and image recognition technology, which is applied in the field of natural products and food and medicine, achieves the effect of simple process, easy operation and wide application

Active Publication Date: 2019-07-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the method is limited to the analysis of one class of sugars

Method used

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  • Thin layer quantitative image recognition detection method for water-soluble natural product
  • Thin layer quantitative image recognition detection method for water-soluble natural product

Examples

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Effect test

Embodiment 1

[0068](+)-Catechin ethanol solution, spotting 0.5 microliters with a capillary to thin-layer chromatography, after the solvent evaporates, use ethyl acetate / ethanol / water / acetic acid (5 / 2.85 / 0.15 / 0.12, v / v / v / v) developer plate to a distance of 20 mm, then allowed to dry, and then re-developed to 40 mm with chloroform / ethyl acetate / formic acid (1 / 1 / 0.03, v / v / v) developer length. Then take it out and wait for the solvent to evaporate to dry, and then stain it with p-methoxybenzaldehyde staining agent, place it on a heating platform at 250°C and heat it for 15 seconds to develop the color. After developing the color of the thin layer, scan it with a computer scanner, and set the resolution to 300dpi. The scanned images were denoised and smoothed with ImageJ, and then the background was removed, and finally quantitatively integrated, and the integrated value was compared with the standard value to obtain the measurement result. The actual value is 1.20 mg / mL of (+)-catechin sol...

Embodiment 2

[0071] The ethanol solution of quercetin was sampled with 0.5 microliters of capillary to thin-layer chromatography, and after the solvent was evaporated, it was washed with ethyl acetate / ethanol / water / acetic acid (5 / 2.85 / 0.15 / 0.12, v / v / v / v ) on the developer plate to a distance of 20 mm, then dried, and then re-developed to a length of 40 mm with chloroform / ethyl acetate / formic acid (1 / 1 / 0.03, v / v / v) developer. Then take it out and wait for the solvent to evaporate to dry, and then stain it with p-methoxybenzaldehyde staining agent, place it on a heating platform at 250°C and heat it for 15 seconds to develop the color. After developing the color of the thin layer, scan it with a computer scanner, and set the resolution to 300dpi. The scanned images were denoised and smoothed with ImageJ, and then the background was removed, and finally quantitatively integrated, and the integrated value was compared with the standard value to obtain the measurement result. The quercetin eth...

Embodiment 3

[0073] With the ethanol solution of tea saponin, spot 0.5 microliters to thin-layer chromatography with a capillary tube and wait for the solvent to evaporate, then use ethyl acetate / ethanol / water / acetic acid (5 / 2.85 / 0.15 / 0.12, v / v / v / v) The developer plate was developed to a distance of 20 mm, then allowed to dry, and then re-developed to a length of 40 mm with chloroform / ethyl acetate / formic acid (1 / 1 / 0.03, v / v / v) developer. Then take it out and wait for the solvent to evaporate to dry, and then stain it with p-methoxybenzaldehyde staining agent, place it on a heating platform at 250°C and heat it for 15 seconds to develop the color. After developing the color of the thin layer, scan it with a computer scanner, and set the resolution to 300dpi. The scanned images were denoised and smoothed with ImageJ, and then the background was removed, and finally quantitatively integrated, and the integrated value was compared with the standard value to obtain the measurement result. For...

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Abstract

The invention discloses a thin layer quantitative image recognition detection method for a water-soluble natural product. The method comprises the steps that (1) a sample to be detected containing thewater-soluble natural product carries out spotting on a thin layer chromatography plate; (2) a first developing agent and a second developing agent are used to carry out gradual thin layer chromatography developing on the spotted thin layer chromatography plate, wherein the first developing agent is a developing system composed of ethyl acetate / ethanol / water / acetic acid, and the second developingagent is a developing system composed of chloroform / ethyl acetate / formic acid; (3) a dye system is used to develop the developed thin layer chromatography plate; and (4) image acquisition is carriedout on the developed thin-layer chromatography plate, and according to the relationship between an image and the content of the water-soluble natural product, the content of the water-soluble naturalproduct is acquired. The method provided by the invention is simple to operate, does not require a dedicated instrument, has the advantages of good quantitative feature, high accuracy and fast testing, and can simultaneously measure multiple samples.

Description

technical field [0001] The invention belongs to the field of natural products and food and medicine, and in particular relates to a thin-layer quantitative image recognition and detection method for water-soluble natural products. Background technique [0002] Water-soluble natural products include flavonoids, flavonoid glycosides, anthocyanins, and saponins, which are important active ingredients in plants. Flavonoids, flavonoid glycosides, and anthocyanins water-soluble natural products have excellent antioxidant effects, and can effectively balance free radicals in the human body by scavenging active oxygen species or their reactions to generate stable compounds, and their metal ion chelating ability can terminate free radicals. base chain reaction. A large number of in vivo and in vitro experiments and epidemiological data show that saponin water-soluble natural products have biological and pharmacological effects such as anti-inflammation, lipid-lowering, anti-diabetes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/95
CPCG01N30/90G01N30/95
Inventor 刘松柏许璐靖束彤
Owner ZHEJIANG UNIV
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