Fucus oligosaccharide and preparation method and application thereof

A technology of fucoidan oligosaccharides and fucoidan, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of low absorption rate and bioavailability, and achieve good moisturizing effect and high fat content. Acidase inhibitory activity, high application value effect

Active Publication Date: 2019-07-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the huge molecular weight of fucoidan, generally ranging from tens of t

Method used

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  • Fucus oligosaccharide and preparation method and application thereof
  • Fucus oligosaccharide and preparation method and application thereof
  • Fucus oligosaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation of fucoidal oligosaccharides

[0062] Fucoidan was treated with 0.01-0.5M H 2 SO 4 Prepare a fucoidan solution with a concentration of 0.3% to 1%, put the fucoidan aqueous solution in a water bath at 60 to 80°C, degrade for 3 to 12 hours, and start to degrade at a shaker speed of 50 to 180r / min. After the degradation is complete, Ba(OH) is added to the aqueous solution 2 powder, make the pH of the fucoidan solution to neutral to terminate the reaction. Centrifuge the final solution (5000r / min, 10min), take the supernatant, and use the ultrafiltration membrane system to divide the fucoidan degradation solution into less than 5kDa, 5-10kDa, 10-30kDa, 30-50kDa, 50-100kDa, and greater than The molecular weight segment of 100kDa was separately freeze-dried to obtain fucoidan oligosaccharide powder.

[0063] Set up three experimental groups A1, A2, and A3, and prepare them according to the following conditions:

[0064]

[0065] The degradation p...

Embodiment 2

[0067] Example 2 Preparation of fucoidal oligosaccharides

[0068] The fucoidan is formulated with 0.01-0.5M HCl to form a fucoidan solution with a concentration of 0.3%-1%. Put the fucoidan aqueous solution in a water bath at 90-100°C, degrade it for 3-12 hours, and start the degradation with the rotating speed of the shaker at 50-180r / min. After the degradation is completed, NaOH powder is added to the aqueous solution to make the pH of the fucoidan solution to neutral to terminate the reaction. Centrifuge the final solution (5000r / min, 10min), take the supernatant, and use the ultrafiltration membrane system to divide the fucoidan degradation solution into fractions of less than 5kDa, 5~10kDa, 10~30kDa, 30- 50kDa, 50-100kDa, molecular weight segment greater than 100kDa, through freeze-drying to powder state is fucoid oligosaccharide.

[0069] Set up three experimental groups B1, B2, and B3, and prepare them according to the following conditions:

[0070]

[0071] Acco...

Embodiment 3

[0072] Example 3 Preparation of fucoidal oligosaccharides

[0073] Weigh 1-5g fucoidan respectively, add 25-80ml 90% formic acid, stir and mix, heat and react in a water bath at 60-90°C, react for 3-12h, and remove formic acid with 25-80ml absolute ethanol after the reaction. Using a membrane ultrafiltration system with a molecular weight cut-off of 5000Da, 10000Da, 30000Da, 50000Da, and 1000000Da, the fucoidan degradation solution is divided into molecular weight segments less than 5kDa, 5-10kDa, 10-30kDa, 30-50kDa, 50-100kDa, and greater than 100kDa. Fucoidal oligosaccharides are obtained by freeze-drying to a powder state.

[0074] Set up three experimental groups C1, C2, and C3, and prepare them according to the following conditions:

[0075]

[0076] The degradation products of fucoidan oligosaccharides prepared by experimental groups B1, B2, and B3 were analyzed by gravimetric analysis and high-performance liquid chromatography. For specific methods, refer to Example...

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Abstract

The invention discloses a fucus oligosaccharide preparation method and prepared fucus oligosaccharide and application thereof. The preparation method includes: S1, adding fucoidin acid solution in concentration of 0.1%-10% into a water bath at 50-100 DEG C, and performing acidolysis for 2-12h under an oscillating condition; S2, after degradation, adding Ba(OH)2 or NaOH powder into the solution, and after pH of the fucoidin solution is neutral, terminating reaction to obtain degradation liquid; S3, centrifuging the degradation liquid for 10min at a rotating speed of 5000r/min, and collecting supernatant; S4, subjecting the supernatant to staged treatment by an ultrafiltration membrane system to obtain degradation products in multistage molecular weight ranges, and respectively subjecting the degradation products to freeze drying to obtain lyophilized powder namely the fucus oligosaccharide. The fucus oligosaccharide prepared according to the method is high in yield, high in skin whitening activity, excellent in moisturizing, bactericidal and antibacterial effect, applicable to preparation of moisturizing cosmetics, skin whitening and freckle removing cosmetics, anti-aging cosmeticsand antibacterial and anti-inflammation cosmetics and high in application value.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a fucoidal oligosaccharide and its preparation method and application. Background technique [0002] Since ancient times, Chinese people have always had the aesthetic concept of "one white covers three ugliness". People's pursuit of fair and moist skin has never stopped. In recent years, with the development of internationalization, the market of whitening and freckle-removing cosmetics has become increasingly active. Product sales are increasing day by day, and it has become one of the mainstream varieties of skin care cosmetics. The development of modern whitening cosmetics in China has gone through different stages of development. In the future, cosmetics will develop into the stage of "green whitening". People pursue natural green functional ingredients. They not only have whitening effects, but also provide nutrition for the skin. Improve cell vitality and self-repair ability, thus...

Claims

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Application Information

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IPC IPC(8): C07H11/00C07H1/00A61K8/60A61Q17/00A61Q19/02A61Q19/00A61Q19/08
CPCA61K8/60A61K2800/782A61Q17/005A61Q19/00A61Q19/02A61Q19/08C07H1/00C07H11/00
Inventor 王莹寇铃赟牛德军
Owner QINGDAO AGRI UNIV
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