Applications of golden camellia extract for improving anti-glycation activity and inhibiting cell fat accumulation

A golden camellia tea and extract technology, which is applied in the field of golden flower tea extract to enhance anti-glycation activity and inhibit cell fat accumulation, can solve problems such as harmful human health and high product price.

Pending Publication Date: 2019-07-09
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the products currently used for fat loss and anti-glycation are made of chemical ingredients. Long-term use is not only harmful to human health, but also these products are often expensive and cannot be afforded by ordinary users

Method used

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  • Applications of golden camellia extract for improving anti-glycation activity and inhibiting cell fat accumulation
  • Applications of golden camellia extract for improving anti-glycation activity and inhibiting cell fat accumulation
  • Applications of golden camellia extract for improving anti-glycation activity and inhibiting cell fat accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1. Preparation of Camellia Camellia Extract

[0029] First, crush Camellia chrysantha from Guangzhou, China into fragments less than 0.5 cm in size, then take the crushed Camellia chrysantha at 80±10°C, extract solvent to Camellia chrysantha at a volume ratio of 5-20:1-5 The extraction is carried out for 0.5-2 hours to obtain a crude extract, wherein the extraction solvent is water, alcohols, aqueous alcohols or a combination thereof. Then, cool to room temperature, then centrifuge the crude extract with 5000rpm for 10 minutes and deslagging and filter, and collect the supernatant. Afterwards, the supernatant was filtered with a 400-mesh filter to obtain a filtrate, and then the filtrate was concentrated under reduced pressure at 55±10° C. to obtain a concentrated product. Next, spray-dry the concentrated product to obtain Camellia japonica extract.

Embodiment 2

[0030] Example 2. Efficacy Evaluation of Camellia Camellia Extract in Anti-Glycation

[0031] This example is to test the anti-glycation activity of the Camellia camellia extract of the present invention. Therefore, the saccharification activity is quantified by inhibiting the efficiency of D-fructose from saccharifying collagen. First, take 0.2mL of water as a control group, or take 0.2mL of Camellia camellia extract of the present invention with a concentration of 100mg / mL, 10 mg / mL, 5mg / mL, 1mg / mL and 0.5mg / mL as an experiment respectively. Group 1~experimental group 5, add 0.2mL NaN containing 0.06% 360mg / mL collagen solution (prepared with 200mM sodium phosphate buffer, pH 7.4), and mixed evenly with 0.2mL of 1.5M D-fructose (prepared with 200mM sodium phosphate buffer, pH 7.4), and took out 0.1mL of the mixed solution was used as the origin product, and the remaining mixed solution was reacted at 50°C for 24 hours, and then 0.1mL was taken out as the end product, and th...

Embodiment 3

[0034] Example 3. Efficacy Evaluation of Camellia Camellia Extract in Inhibiting Fat Accumulation

[0035] In this example, mouse bone marrow stromal cells (bone marrow stromal cells) were used to test the efficacy of the Camellia camellia extract of the present invention in inhibiting fat accumulation. The mouse bone marrow stromal cells were purchased from the American Type Culture Collection (USA ), No. CRL-2749 TM . The cells were cultured in pre-adipocyte expansion medium (Pre-adipocyte Expansion Medium) before differentiation, which contained 90% minimum essential medium Alpha medium (minimum essential medium alpha medium, purchased from Gibco, the United States, 12100- 046), 20% fetal bovine serum (Fetal Bovine Serum, purchased from Gibco, the U.S.), and adding 1% penicillin / streptomycin (Penicillin-streptomycin, purchased from Gibco, the U.S.); Medium) to differentiate mouse bone marrow stromal cells containing 90% minimal essential medium Alpha medium, 20% fetal c...

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Abstract

The invention relates to a plant extract, in particular to application of a golden camellia extract to improvement of anti-glycation activity and inhibition of cell fat accumulation. The invention provides an application of a golden camellia extract in preparation of a composition for improving anti-glycation and inhibiting cell fat accumulation. The golden camellia extraction can improve the ability of removing highly-glycated end products, inhibit fat accumulation, reduce the fat content in cells and achieve the effect of reducing fat.

Description

technical field [0001] The present invention relates to plant extracts, in particular to the use of a Camellia japonica extract for improving anti-glycation activity and inhibiting cell fat accumulation. Background technique [0002] Fat is an essential component in the human body, but excessive fat content will cause damage to the human body. [0003] In addition, Taiwan, China has now become the "fattest region in Asia". Among the residents over 18 years old, 51.1% of males are overweight, and 35.8% of females are overweight, and the proportion is still rising. The overweight age group is also declining year by year, and the proportion of overweight primary and middle school students has risen to more than 25%, ranking the eighth highest in the world. Therefore, obesity is an urgent problem to be solved. [0004] In addition, glycation is related to skin and internal aging, and it is also related to diabetes, which is very harmful to the health of Chinese people. The es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P3/04A23L33/105
CPCA61K36/82A61P3/04A23L33/105A61K2236/331A61K2236/333A23V2002/00A23V2200/332A23V2250/21
Inventor 林咏翔
Owner TCI CO LTD
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