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Method for recovering proteins in protein wastewater

A protein wastewater and protein technology, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of limited acid adjustment ability, high recycling cost, unfavorable industrialization, etc., and achieve high production safety and low cost , Improve the effect of removal rate

Active Publication Date: 2019-07-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a method for recovering protein in protein wastewater, so as to solve the problems existing in the prior art such as limited ability to adjust acid, high recovery cost, complex process and unfavorable industrialization

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Effect test

Embodiment 1

[0049] The present embodiment reclaims protein from protein wastewater by the following methods:

[0050] Select a kind of waste water produced after processing pollock surimi. The initial pH value of the surimi wastewater is about 7.0. According to the analysis of protein components, the protein concentration of the waste water is 7.6 mg / mL after centrifugation pretreatment, and the isoelectric point is relatively dense. Concentrated in the vicinity of 6.40, 5.10 and 4.30, according to the analysis results, the proteins are divided into three levels according to the isoelectric point of the protein from the largest to the smallest. Among them, recycling equipment such as figure 1 shown.

[0051] First-level isoelectric precipitation, adding acetic acid to the acid-carrying kettle, passing air through the pressure buffer steel cylinder, and feeding air into the acid-carrying kettle, the safety valve of the acid-carrying kettle is kept at an absolute pressure of 1.5atm, and th...

Embodiment 2

[0055] The present embodiment reclaims protein from protein wastewater by the following methods:

[0056] Select a kind of wastewater produced after processing blue cod surimi. The initial pH value of the surimi wastewater is about 7.2. According to the analysis of protein components, the protein concentration of the wastewater after centrifugation pretreatment is 7.1mg / mL, and the isoelectric point is relatively dense. Concentrated in the vicinity of 6.45, 5.20 and 3.25, according to the analysis results, the proteins are divided into three levels according to the isoelectric point of the protein from the largest to the smallest.

[0057] First-level isoelectric precipitation, add acetic acid to the acid-carrying kettle, pass air through the pressure buffer steel cylinder, and feed air into the acid-carrying kettle. The acid-adjusting kettle maintains normal temperature and pressure, and the pH value of the acid-adjusting kettle reaches 6.45±0.05. After the gas in the acid a...

Embodiment 3

[0061] The present embodiment reclaims protein from protein wastewater by the following methods:

[0062] Select a kind of wastewater produced after processing mixed surimi. The initial pH value of the surimi wastewater is about 7.2. According to the analysis of protein components, the protein concentration of the wastewater after centrifugation pretreatment is 8.9 mg / mL, and the isoelectric point is relatively dense. Concentrated in the vicinity of 6.40, 5.15, 4.30 and 3.25, according to the analysis results, the proteins are divided into three levels according to the isoelectric point of the protein from the largest to the smallest.

[0063] First-level isoelectric precipitation, adding acetic acid to the acid-carrying kettle, passing air through the pressure buffer steel cylinder, and passing air into the acid-carrying kettle, the safety valve of the acid-carrying kettle is kept at an absolute pressure of 1.3atm, and the temperature of the acid-carrying kettle is 25°C. The ...

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Abstract

The invention provides a method for recovering proteins in protein wastewater. The method comprises the steps of introducing an air-carried volatile acid into the protein wastewater to adjust the pH value to a protein isoelectric point; performing precipitation to recover the proteins from the protein wastewater. According to the provided method, the air-carried volatile acid is used as an acid adjusting means, the pH value of the wastewater with high protein concentration can be adjusted to the isoelectric point of each protein under low pressure through air pressure, the carrying temperature, the carrying time and the like, the proteins with different isoelectric points in the wastewater are separately precipitated and removed, the protein removal rate is increased, the total protein recovery rate can reach about 95% or above, industrial production is facilitated, and the method has a high value in practical application.

Description

technical field [0001] The invention belongs to the field of biochemical separation, and relates to a method for recovering protein in protein wastewater. Background technique [0002] Most of the high-concentration protein wastewater adopts traditional biological treatment methods, but whether it is aerobic treatment or anaerobic treatment, there are problems such as the generation of sludge pollutants and odor, long treatment cycle, and complicated operation. In particular, biological methods not only cannot effectively recover proteins in high-protein wastewater, but also produce secondary pollutants, resulting in waste of resources. Therefore, in the treatment of high-protein wastewater, many researchers have tried various methods to recover protein, such as membrane separation, flocculation-sedimentation, and ohmic heat treatment. However, there are problems such as membrane fouling, long processing time and high cost, making it difficult for industrial application. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/30
CPCC07K1/30
Inventor 齐祥明褚灵燕黄文灿毛相朝
Owner OCEAN UNIV OF CHINA
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