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A kind of loading device and loading method of cell stretching force

A loading device and tension technology, which is applied in the field of cell mechanics, can solve the problems of high maintenance difficulty, many components, and large footprint of the instrument, and achieve the effect of small footprint, few components, and favorable price

Active Publication Date: 2021-03-23
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The cell mechanical force loading device in the prior art is to cause the membrane to change by pumping the elastic membrane into a vacuum state, and then make the cells attached to the elastic membrane grow on the elastic membrane to be subjected to mechanical force to study the stretching force. The loaded force is accurate and controllable, but this device is limited in practicability due to its high price, poor portability, large footprint, many components, and high difficulty in instrument maintenance, so it is difficult to promote in small and medium-sized laboratories in China use

Method used

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  • A kind of loading device and loading method of cell stretching force
  • A kind of loading device and loading method of cell stretching force
  • A kind of loading device and loading method of cell stretching force

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A loading device for cell tension, such as Figure 1-7 As shown, it includes a cupping vacuum suction device, an elastic membrane culture plate and a supporting device. The cupping vacuum suction device includes a vacuum device, a rubber tube and a vacuum suction head, and the vacuum suction head includes an integrally formed suction A suction head and a suction chamber, and the suction head is provided with a suction hole, one end of the rubber tube is detachably connected with the vacuum equipment, the other end is inserted into the suction hole, and connected with the The suction head is detachably connected; the elastic membrane culture plate is provided with several culture tanks, and the bottom of each culture tank is an elastic membrane made of elastic silicone membrane; the supporting device can be placed on the The spring support device or the sterile cotton support device in the suction chamber mentioned above.

[0033] The model of the cupping vacuum suction...

Embodiment 2

[0036] A method for utilizing upper-loaded cell tension, comprising the steps of:

[0037] Step 1, pre-planting adherent cells in the elastic membrane culture plate;

[0038] Step 2, placing the vacuum suction head under the elastic membrane culture plate, and making the suction chamber completely cover any elastic membrane on the elastic membrane culture plate;

[0039] Step 3, one end of the rubber tube is communicated with the suction head of the vacuum suction head, and the other end is connected with the vacuum pumping equipment, and the applied force of -538mmHg is set on the vacuum pumping equipment (the secondary suction of the cupping vacuum equipment), After adding force for 1.5s, close the vent port of the vacuum equipment, open the vacuum equipment, and start the shaft vacuum;

[0040] Step 4. After reaching the time for adding force, immediately open the air vent and pour the quick-setting dental cement into the elastic membrane culture plate, and let it stand un...

Embodiment 3

[0042] A method for utilizing upper-loaded cell tension, comprising the steps of:

[0043] Step 1, pre-planting adherent cells in the elastic membrane culture plate;

[0044] Step 2, placing the supporting device in the suction cavity of the vacuum suction head to form a supporting suction device, and placing the supporting suction device under the elastic membrane culture plate, And make the suction chamber supporting the suction device completely cover any elastic membrane on the elastic membrane culture plate; the supporting device is a spring supporting device.

[0045] Step 3, one end of the rubber tube is communicated with the suction head of the vacuum suction head, and the other end is connected with the vacuum pumping equipment, and the applied force of -538mmHg is set on the vacuum pumping equipment (the secondary suction of the cupping vacuum equipment), After adding force for 1.5s, close the vent port of the vacuum equipment, open the vacuum equipment, and start t...

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Abstract

The invention relates to the field of cytomechanics, in particular to a loading device based on cell stretch stress. The loading device is characterized by comprising a traditional Chinese medicine cupping vacuum suction device, an elastic film culture board and supporting devices. Different suction force and acting time durations are set on the vacuum suction device. Different degrees of stretchstress are exerted on cells attached to an elastic film through the different supporting device for research. The elastic film deformation and deformation rate caused by the different supporting devices under the effect of same suction force for different time durations are measured at the same time, and therefore the deformation conditions formed after the cells attached to the elastic film bearthe stretch stress are researched.

Description

technical field [0001] The invention belongs to the field of cell mechanics, and specifically relates to a cell tension loading device and a loading method thereof. Background technique [0002] In organisms, biological behaviors such as cell growth, proliferation, and secretion are inseparable from the mechanical environment in which they live. Cells can perform their functions normally in a normal physiological and mechanical environment. However, when the mechanical environment around cells changes, various functions of cells also change. Many scholars have applied cell mechanics experiment methods to apply stress and strain stimuli simulating the in vivo environment to tissue cells cultured in vitro through mechanical force loading devices, combined with morphological and molecular biology testing techniques, and carried out a large number of cell growth under various stress environments. , metabolism, and changes in the expression of intracellular proteins and cytokine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M23/48C12M33/04C12M45/02
Inventor 董红梅邱明洁吴嘉燕吕斌麻婧邓末宏黄伟胜
Owner HUAZHONG UNIV OF SCI & TECH