The invention relates to an avicennia mavina kernel mooncake filling. The avicennia mavina kernel mooncake filling is prepared from the following raw materials in parts by weight: 80-125 parts of avicennia mavina kernels, 25-60 parts of walnut kernels, 20-55 parts of Indian almond kernels, 75-120 parts of sesame seeds, 75-115 parts of apricot kernels, 60-95 parts of melon seed kernels, 10-22 parts of dried oranges, 20-45 parts of preserved white gourd strips, 35-70 parts of pork, and 25-35 parts of peanut oil. The invention further discloses a preparation method of the avicennia mavina kernel mooncake filling. According to the mooncake filling, the avicennia mavina kernels, the Indian almond kernels and the walnut kernels are used as main materials, and through cooperation of the sesame seeds, the melon seed kernels, the apricot kernels and the dried oranges, the effects of the moon cake filling for clearing heat for detumescence, promoting urination, promoting urination, cooling blood, relieving inflammation or internal heat, reducing blood lipid, removing toxicity, expelling toxin, invigorating the spleen and benefiting the stomach are greatly improved, the content of fat and the calorie in the mooncake filling can also be effectively reduced, the mooncake filling is free from any preservatives, body health is facilitated, a processing and using method for seasonal harvest of the avicennia mavina kernels is provided, and the avicennia mavina kernel mooncake filling fills the blank for making mooncakes from the avicennia mavina kernels.