Multiple gene detection kit for guiding antipsychotic drugs and using method thereof

Abstract

The invention discloses a multiple gene detection kit for guiding antipsychotic drugs and a using method thereof. The kit adopts multiple PCR combined with a fragment length / mass analysis method and synchronously and qualitatively detects 7 nucleotide polymorphism (SNP) locus on 6 genes including CYP2D6, MC4R, CYP1A2, CYP3A5, EPM2A, and SLC6A2 which are related to antipsychotic drug metabolism, transport and target action. The method comprises the detection steps of (1) collecting oral exfoliated cells and storing on a cell collection card or collecting blood samples and extracting nucleic acids; (2) performing multiple PCR amplification using the cell collection card or nucleic acid as described in step 1 as formwork; (3) according to PCR product fragment length / mass, simultaneously separating into 7 SNP loci, 3 human DNA internal reference genes and 1 PCR reaction internal reference PCR product; (4) analyzing results and interpreting. The kit has the advantages of fast performance, high sensitivity, good repeatability, strong specificity, high throughput and low cost.

Description

technical field [0001] The invention relates to a multiple gene detection kit, in particular to a multiple gene detection kit for guiding the use of antipsychotic drugs and a method for using the same. Background technique [0002] With the rapid development of my country's social economy and the increasing pressure of competition, the incidence of mental illness is increasing year by year. The prevalence rate of mental illness in our country is about 17%. Schizophrenia is one of the most serious mental illnesses, affecting approximately 1% of the population worldwide. Schizophrenia has a variety of complex clinical manifestations, mainly characterized by changes in basic personality, incoordination between thinking, emotion, and behavior, and separation of mental activities from reality. Schizophrenia not only brings great pain to the patients themselves and their families, but also brings a heavy economic burden to the family, the medical system and the whole society. ...

AI Technical Summary

Problems solved by technology

However, the Sanger sequencing method is complex and expensive

When there are many sequencing sites, it needs to be sequenced one by one, which takes a long time and the cumulative price is relatively expensive

The next-generation sequencing technology realizes sequencing while synthesizing, which has the advantages of high throughput and high efficiency. However, the next-generation sequencing platform is expensive and has low popularity, and its clinical application as a SNP detection technology is not mature enough.

[0014] Due to the large number of gene SNPs related to antipsychotic medication guidance, the above techniques have obvious limitations, so it is difficult to apply multiple gene detection for antipsychotic medication guidance

[0015] At present, there are no reports in China on the multiplex gene detection scheme based on multiplex PCR and CE separation technology for antipsychotic medication guidance

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