Method of detecting erythrothioneine
A technology of ergothioneine and samples, which is applied in the field of chemical analysis, can solve problems such as chromatographic peak shape, difficulty in ensuring accuracy and stability, and collapse of chromatographic column fillers, and achieve long use time, short detection time and high accuracy. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment approach
[0035] According to the present invention, as long as there is an ergothioneine chromatographic peak on the high-pressure liquid chromatography analysis spectrum, the existence of ergothioneine in the sample to be tested can be known. In the method of the present invention, the peak-out time of ergothioneine is the 5th to 6th minute. Further measure the peak area of the ergothioneine chromatographic peak according to the integral method and then it is possible to quantify the ergothioneine content in the sample to be tested. Therefore, according to a specific implementation of the present invention, the method also includes calculating the peak area corresponding to the 5th to 6th minute on the chromatogram obtained by high-pressure liquid chromatography analysis, to carry out the ergothioneine in the described sample to be tested. Quantitative. Quantification can be performed by means of a standard curve, and the method of drawing a standard curve is well known to those sk...
preparation example 1
[0047] Preparation of reference substance solution: Accurately weigh 10 mg of ergothioneine reference substance, add pure water in a 10ml volumetric flask to form a reference substance stock solution with a concentration of 1000 mg / L. Then draw an appropriate amount of stock solution, add pure water to make a solution with a concentration of 50mg / L, 100mg / L, 200mg / L, 300mg / L, and 400mg / L, and filter through a 0.22μm microporous membrane to obtain reference substances with different concentrations solution.
[0048] Test solutions 1-4 with other concentrations were obtained in the same manner as above.
preparation example 2
[0050] Preparation of fermentation broth of Pleurotus otreatus CGMCC No.6232 mycelium.
[0051] Slant medium: PDA medium (Becton, Dickinson and Company).
[0052] Liquid seed medium (g / L): corn flour 30g / L, bean cake powder 15g / L, α-amylase 54U / L, KH 2 PO 4 3g / L, MgSO 4 ·7H 2 O 1.5g / L, the rest is water, sterilized at 121°C for 20 minutes, and the liquid volume in a 500mL Erlenmeyer flask is 150mL.
[0053] Liquid fermentation medium (g / L): glycerol 68g / L, casein peptone 45g / L, KH 2 PO 4 3g / L, MgSO 4 ·7H 2 O1.5g / L, cysteine 0.9g / L, methionine 2.1g / L, the rest is water, sterilized at 121°C for 20min, and the liquid volume in a 500mL Erlenmeyer flask is 150mL.
[0054] The bacterial lawn of strain CGMCC No.6232 from the slant medium was picked and inserted into the seed medium, and cultured on a shaker at 25° C. at 150 rpm for 4 days to obtain a seed liquid. The seed solution was added to the fermentation medium with an inoculation amount of 5% by volume, and cultur...
PUM
| Property | Measurement | Unit |
|---|---|---|
| wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More