Method for purifying and concentrating composite biological active factors and concentrated liquid thereof

Abstract

The present invention discloses a method for purifying and concentrating composite biological active factors and concentrated liquid thereof. An organic solvent is added into biological tissue cultureliquid containing the composite biological active factors, the biological tissue culture liquid is continuously stirred to be uniform to obtain biological tissue mixed liquid, the obtained biologicaltissue mixed liquid is put into a pressure processor, a temperature-rising treatment is conducted, rapid depressurizing is conducted to normal pressure, besides, the biological tissue mixed liquid istaken out, centrifuging is conducted, after the centrifuging, filtration is conducted to remove residual biological tissue impurities in the biological tissue mixed liquid to obtain clarified liquidof the biological tissue mixed liquid, besides, an ultrafiltration membrane is used to filter out the clarified liquid to obtain a biological tissue ultrafiltrate containing the active factors, and besides, the ultrafiltrate is further purified and concentrated to obtain the concentrated liquid containing the composite biological active factors. The concentrated liquid containing the composite biological active factors can accelerate repair after a skin surgery and also has functions of smoothing fine lines, delaying skin aging, etc.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for purifying and concentrating complex bioactive factors and a concentrated solution thereof. Background technique [0002] Active factors, also known as growth factors, active growth factors, and active cytokines, are a class of polypeptide substances that regulate cell growth and other cell functions by binding to specific, high-affinity cell membrane receptors. Active factor is a combination of various amino acids that can regulate cell growth and differentiation, also known as "peptide". Peptide is a precise protein fragment, so active factor is also called active protein factor. It is distributed in various tissues of the human body, mainly In platelets and various adult and embryonic tissues and most cultured cells. The content is extremely small but it is very important to the regulation function of human cells. From the perspective of molecular structur...

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Problems solved by technology

[0003] With the development of society, the use of compound active factors in medical treatment and skin care is getting higher and higher, and the extraction, concentration and preparation of concentrates containing compound active factors generally require the concentration and extraction of tissue culture fluids containing compound active factors. However, the existing concentrated extraction method has high cost and low efficiency, and the effective activity of the composite active factor cannot be guaranteed during the extraction process, and the utilization rate is poor.