Method for purifying and concentrating composite biological active factors and concentrated liquid thereof

A technology of bioactive factors and growth factors, which is applied in the field of purification and concentration of compound bioactive factors and its concentrate, can solve the problems of inability to guarantee the effective activity of compound active factors, high cost of concentration and extraction, poor utilization rate, etc., and achieve delay Effect of skin aging, guaranteed nutrition and functional ingredients, low cost

Inactive Publication Date: 2019-07-19
雷艳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the development of society, the use of compound active factors in medical treatment and skin care is getting higher and higher, and the extraction, concentration and preparation of concentrates containing compound active factors generally require the concentration and extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] The present invention provides a method for purifying and concentrating composite biologically active factors and a concentrated solution thereof, including the following steps:

[0021] S1, adding the biological tissue culture medium containing the compound biologically active factors to the organic solvent, and continuously stirring until uniform, to obtain the biological tissue mixture;

[0022] S2: Put the biological tissue mixture obtained in S1 into a pressure processor, perform a pressure increase treatment, pressurize to 120 MPa, and hold for 5 minutes, then quickly release the pressure to normal pressure, and take out the biological tissue mixture;

[0023] S3: Add the biological tissue mixture solution taken out in S2 to the centrifuge and perform centrifugation. After the centrifugation is completed, filter through a 100nm filter medium to remove residual biological tissue impurities in the biological tissue mixture solution to obtain a biological t...

Example Embodiment

[0032] Example 2

[0033] A method for purification and concentration of compound biologically active factors and a concentrated solution thereof include the following steps:

[0034] S1, adding the biological tissue culture medium containing the compound biologically active factors to the organic solvent, and continuously stirring until uniform, to obtain the biological tissue mixture;

[0035] S2: Put the biological tissue mixture obtained in S1 into a pressure processor, perform pressure-increasing treatment, pressurize to 400MPa, and hold for 8 minutes, then quickly release the pressure to normal pressure, and take out the biological tissue mixture;

[0036] S3: Add the biological tissue mixture solution taken out in S2 to the centrifuge and perform centrifugation. After the centrifugation is completed, filter through a 100nm filter medium to remove residual biological tissue impurities in the biological tissue mixture solution to obtain a biological tissue mixture The clear liqui...

Example Embodiment

[0045] Example 3

[0046] A method for purification and concentration of compound biologically active factors and a concentrated solution thereof include the following steps:

[0047] S1, adding the biological tissue culture medium containing the compound biologically active factors to the organic solvent, and continuously stirring until uniform, to obtain the biological tissue mixture;

[0048] S2: Put the biological tissue mixture obtained in S1 into a pressure processor, perform pressure-increasing treatment, pressurize to 700 MPa, and hold for 10 minutes, quickly release the pressure to normal pressure, and take out the biological tissue mixture;

[0049] S3: Add the biological tissue mixture solution taken out in S2 to the centrifuge and perform centrifugation. After the centrifugation is completed, filter through a 100nm filter medium to remove residual biological tissue impurities in the biological tissue mixture solution to obtain a biological tissue mixture The clear liquid o...

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PUM

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Abstract

The present invention discloses a method for purifying and concentrating composite biological active factors and concentrated liquid thereof. An organic solvent is added into biological tissue cultureliquid containing the composite biological active factors, the biological tissue culture liquid is continuously stirred to be uniform to obtain biological tissue mixed liquid, the obtained biologicaltissue mixed liquid is put into a pressure processor, a temperature-rising treatment is conducted, rapid depressurizing is conducted to normal pressure, besides, the biological tissue mixed liquid istaken out, centrifuging is conducted, after the centrifuging, filtration is conducted to remove residual biological tissue impurities in the biological tissue mixed liquid to obtain clarified liquidof the biological tissue mixed liquid, besides, an ultrafiltration membrane is used to filter out the clarified liquid to obtain a biological tissue ultrafiltrate containing the active factors, and besides, the ultrafiltrate is further purified and concentrated to obtain the concentrated liquid containing the composite biological active factors. The concentrated liquid containing the composite biological active factors can accelerate repair after a skin surgery and also has functions of smoothing fine lines, delaying skin aging, etc.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for purifying and concentrating complex bioactive factors and a concentrated solution thereof. Background technique [0002] Active factors, also known as growth factors, active growth factors, and active cytokines, are a class of polypeptide substances that regulate cell growth and other cell functions by binding to specific, high-affinity cell membrane receptors. Active factor is a combination of various amino acids that can regulate cell growth and differentiation, also known as "peptide". Peptide is a precise protein fragment, so active factor is also called active protein factor. It is distributed in various tissues of the human body, mainly In platelets and various adult and embryonic tissues and most cultured cells. The content is extremely small but it is very important to the regulation function of human cells. From the perspective of molecular structur...

Claims

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Application Information

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IPC IPC(8): C07K14/485C07K14/515C07K14/50C07K14/495C07K14/475C07K14/49C07K14/505C07K14/53C07K14/54C07K1/34
CPCC07K14/475C07K14/485C07K14/49C07K14/495C07K14/50C07K14/503C07K14/505C07K14/515C07K14/53C07K14/54
Inventor 雷艳
Owner 雷艳
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