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Tilapia mossambica fatty liver damage model and building method and application thereof

A fatty liver injury and construction method technology, applied in the field of aquaculture, can solve problems such as the occurrence and development mechanism of fatty liver disease in fish that have not been paid attention to, the lack of close integration with actual production, mechanical liver injury, etc., and achieve good application prospects. The effect of short molding time and easy control of ingredients

Inactive Publication Date: 2019-07-23
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deficiency of this method is: 1) this method is an injection method, very easily causes fish mechanical liver damage, and the stress to fish is bigger simultaneously; 2) this method easily causes fish death (85% of the highest survival rate) ; 3) This model is mainly liver damage caused by hepatic metabolic disorder caused by chemical substances (thioacetamide), and does not pay attention to the occurrence and development mechanism of the main fatty liver disease (nutritional fatty liver) in fish. The actual production is not closely integrated

Method used

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  • Tilapia mossambica fatty liver damage model and building method and application thereof
  • Tilapia mossambica fatty liver damage model and building method and application thereof
  • Tilapia mossambica fatty liver damage model and building method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] (1) Experimental design

[0027] Healthy and uninjured tilapia (initial weight 142.15±7.55g) were selected and randomly divided into two groups: model group and blank control group, with 30 fish in each group. After 2 weeks of adaptive feeding, all tilapias began to be formally fed. The model group was fed high-fat diet: 85% tilapia basal feed, 13% lard, 1.5% soybean oil and 0.5% cholesterol. The blank control group was fed with basal feed. The two groups were fed with the same amount, twice a day (9:00 and 16:00), and the daily feeding amount was 2-3% of the total fish body weight, and they were fed continuously for 8 weeks. After 4 weeks of modeling, the tilapia showed symptoms of fatty liver, and after 8 weeks, the tilapia showed obvious fat deposition and liver damage.

[0028] (2) Evaluation method and result analysis of tilapia liver injury

[0029] Evaluation methods include serum biochemical markers, liver lipid levels, and histopathological diagnosis. Afte...

Embodiment 2

[0041] (1) Experimental design

[0042] Based on this animal model, the protective effects of berberine and polydatin on fatty liver injury in tilapia were evaluated. Set up blank control group, model group and drug treatment group (berberine and polydatin treatment group). The blank control group was fed with basal feed; the model group was fed with high-fat feed (85% tilapia basal feed, 13% lard, 1.5% soybean oil and 0.5% cholesterol); the berberine group was fed with 50 mg / kg small The high-fat feed of berberine; the polydatin group was fed with high-fat feed containing 1g / kg polydatin. 30 fish per group, for three parallel replicates. The breeding cycle of this experiment was 8 weeks, and sampling and analysis were carried out at the 8th week.

[0043] (2) Evaluation method and result analysis of the hepatoprotective effect of berberine and polydatin

[0044] Evaluation methods include serum biochemical markers, liver lipid levels, and liver tissue antioxidant levels. ...

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Abstract

The invention discloses a Tilapia mossambica fatty liver damage model and a building method and application thereof. The building method includes: domesticating, preparing high-fat feed, inducing andperforming detection and analysis. Compared with the prior art, the method has the advantages that the high-fat feed used by the method is simple in formula, easy to operate, easy in component control, high in modeling success rate, stable, short in modeling time and promising in application prospect; the animal model built by the method can be used for researching the pathogenesis of fish fatty liver damage caused by various factors and the mutual relation between fat metabolic disorder and liver damage, screening drugs for preventing and treating fish fatty liver and evaluating the pharmacological mechanism of fish liver-protecting drugs.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and relates to a method for constructing a fish injury model, in particular to a tilapia fatty liver injury model and its construction method and application. Background technique [0002] Fatty liver injury is one of the main liver diseases in fish, and it is also a widespread disease that has plagued the aquaculture industry for a long time. There are many inducing factors, such as nutritional imbalance, environmental factor stress, abnormal physiological function, etc. Excessive accumulation of fat and disorder of lipid metabolism lead to liver lesions. Fatty liver disease affects almost all main fish species in my country, and its harm mainly includes reduced growth, feed utilization, immunity, stress tolerance, product sensory, etc., which has a great negative impact on the aquaculture industry. Although after many years of research and prevention and control practice, it has not been f...

Claims

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Application Information

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IPC IPC(8): A01K61/10A23K50/80A23K20/158A23K20/168A61K49/00
CPCA61K49/0008A23K20/158A23K20/168A23K50/80A01K61/10Y02A40/81Y02A40/818
Inventor 贾睿殷国俊杜金梁曹丽萍
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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