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Application of mmu-mir-218-5p in the preparation of drugs for inhibiting embryo implantation

A drug and embryo technology, applied in the application field of mmu-miR-218-5p in the preparation of drugs that inhibit embryo implantation, can solve the problem of window period decline and achieve a significant inhibitory effect

Active Publication Date: 2021-08-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our previous study found that miR-218-5p decreased significantly during the window period of embryo implantation

Method used

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  • Application of mmu-mir-218-5p in the preparation of drugs for inhibiting embryo implantation
  • Application of mmu-mir-218-5p in the preparation of drugs for inhibiting embryo implantation
  • Application of mmu-mir-218-5p in the preparation of drugs for inhibiting embryo implantation

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Experimental program
Comparison scheme
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Embodiment 1

[0025] 1. Small RNA sequencing

[0026] 1. Take materials

[0027] For the ICR mouse strain, select the estrous female mouse and the male mouse to co-cage at 17 o'clock in the evening, and check the plug at 9 o'clock the next morning. If a white plug appears at the vaginal opening of the female mouse, it proves that the female mouse is pregnant, and it is recorded as pregnancy D1 sky.

[0028] Collect endometrial tissue from the first day (D1), fourth day (D4), fifth day implantation site (D5IMS) and non-implantation site (D5IIS) after pregnancy: the collection method is, within the corresponding days, At 10:00 in the morning, the mice were humanely killed by neck dissection, and 75% alcohol was used for disinfection, and then the uterine tissue of the mice was removed, the adherent fat and blood vessels were removed, and the attached blood was washed away, and the mice were separated by extrusion stripping endometrium. The endometrial tissue of the first day (D1), fourth d...

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Abstract

The invention discloses the application of a microRNA—mmu-miR-218-5p in the preparation of medicines for inhibiting embryo implantation. The present invention screens out the microRNA that regulates the uterine receptivity stage and plays a key role in the implantation site through the microarray experiment of microRNA expression profile and bioinformatics method: mmu‑miR‑218‑5p, and it is proved by experiments that mmu‑miR‑218 ‑5p has an extremely significant inhibitory effect on embryo implantation, suggesting that it can be used as a contraceptive drug and provides a new way for new drug screening.

Description

[0001] (1) Technical field [0002] The invention relates to the application of mmu-miR-218-5p in the preparation of medicines for inhibiting embryo implantation. [0003] (2) Background technology [0004] In recent years, the important role of miR-218-5p in the process of cell migration, invasion and microangiogenesis in tumor tissue has gradually attracted attention. The reported literature has confirmed that miR-218-5p is important for the occurrence, growth and spread of tumors. Impact. In gastric cancer, miR-218-5p inhibits the invasion and metastasis of gastric cancer through its target gene ROBO1. miR-218-5p inhibits cell invasion through the CD44-ROCK pathway in oral squamous cell carcinoma cell lines. At the same time, miR-218 can negatively regulate the migration ability and planar lumen formation ability of endothelial cells, thereby affecting the formation of microvessels. Similar to tumor migration and microvessel formation, cell migration, invasion and microve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/713A61P15/18
CPCA61K31/713A61P15/18
Inventor 王争光曹丁壬梁晶婕邱寒峰谭强施爽
Owner ZHEJIANG UNIV