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Fat graft rich in high activity fatty granule cells and adipose-derived stem cells and preparation method and application of fat graft

An adipose stem cell and fat particle technology, which is applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of insufficient self-fat storage, only 10% gel yield, and low SVF-gel absorption rate. Effects of centrifugation times, improving graft survival, and reducing the number of fat boluses

Active Publication Date: 2019-07-23
CHENGDU YUANSHAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After filling in this way, the absorption rate of SVF-gel is low, and the gel yield is only 10% to 15%. Generally, it is only suitable for small-dose filling, and it is difficult to treat patients with large-volume fat grafting or insufficient fat storage. Large scale

Method used

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  • Fat graft rich in high activity fatty granule cells and adipose-derived stem cells and preparation method and application of fat graft
  • Fat graft rich in high activity fatty granule cells and adipose-derived stem cells and preparation method and application of fat graft
  • Fat graft rich in high activity fatty granule cells and adipose-derived stem cells and preparation method and application of fat graft

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The purpose of this example is to select the most suitable liposuction method.

[0042] The fat extraction method of the present embodiment is as follows:

[0043] Negative pressure liposuction machine: use the currently commonly used machine to assist the negative pressure liposuction machine to extract fat;

[0044] Manual extraction: Use a 10mL or 20mL syringe for manual negative pressure liposuction. The extracted fat is left standing at 4°C under sterile conditions to remove water to obtain fat (granular fat). Use 3-5 holes for liposuction with a diameter of 1.2-2mm Needle, liposuction syringe is best to use 10mL or 20mL syringe.

[0045] The fat treatment process and result records of the two groups are shown in Table 1, as follows:

[0046] Table 1

[0047]

[0048]

[0049] The results of the two groups showed that compared with manual extraction, the fat from machine-assisted negative pressure liposuction needs to increase the number of centrifugation ...

Embodiment 2

[0051] The purpose of this example is to examine the effect of mechanical treatment on the viability of cells in fat.

[0052] Each group of fat preparation process of the present embodiment is as follows:

[0053] Control group: common fat treated by centrifugation to remove water;

[0054] Group A: (1) manual negative pressure liposuction with 10mL or 20mL syringe, the extracted fat was left to stand under sterile conditions at 4°C to remove water to obtain fat particles; the aperture of the liposuction needle used was 1.2-2mm, The number of holes in the needle is 3 to 5;

[0055] (2) Add physiological saline twice the volume of the fat particles in step (1), shake and mix well, and centrifuge at 300-500g for 3 minutes. After centrifugation, the fat is divided into the upper fat layer and the lower water layer, discard the lower water layer and tube The basal erythrocytes are precipitated to obtain the fat layer;

[0056] (3) The fat layer obtained in step (2) is divided ...

Embodiment 3

[0078] The purpose of this example is to detect the effect of mechanical treatment on the activity of adipose stem cells in fat grafts.

[0079] The specific operation is as follows:

[0080] (1) Digest and collect the SVF-cells obtained from the four groups of fats in Example 2, remove red blood cells, and press 2×10 6 Cells / mL were resuspended and cultured in 6-well plate culture flasks, 1 mL / well, and about 2×10 cells were inoculated in each well. 6 cells. After 24 hours of attachment, observe the general adhesion of the cells in the four groups of flasks.

[0081] (2) Suck off the supernatant in the culture flask, add normal saline to wash twice, add 0.25% trypsin to digest in each bottle, neutralize and digest, centrifuge at 400g for 3min, discard the supernatant to obtain cell pellet;

[0082] (3) Prepare the cell pellet as a cell suspension, add 0.4% trypan blue to the cell suspension at a volume of 9:1 for staining (the volume ratio of cell suspension: 0.4% trypan b...

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Abstract

The invention belongs to the technical filed of fat transplantation, and particularly relates to a fat graft rich in high activity fatty granule cells and adipose-derived stem cells and a preparationmethod and application of the fat graft. The method comprises the following steps of performing manual negative-pressure liposuction with an injector, performing centrifugation on obtained fat to obtain a fat layer, enabling the lower layer of the fat layer to be digested to prepare cell suspension, pushing the upper layer to obtain an intermediate layer for transplantation, and finally, uniformlymixing the cell suspension with the intermediate layer to obtain the fat graft rich in high activity fatty granule cells and adipose-derived stem cells. The fat graft has the advantages that comparedwith conventional SVF-gel, the fat graft prepared by the method is higher in yield, and contains massive high-activity granule adipocyte, SVF-cells and adipose-derived stem cells, the concentration of the cells is 5-10 times of that of normal fat tissues, the survival rate of the transplantation fat can achieve 70%, the transplantation fat is soft and uniform in texture, and the probability of adverse reactions of oily tumor masses, tumor tubercles and the like is notably reduced.

Description

technical field [0001] The invention belongs to the technical field of fat transplantation, and relates to a method for processing fat tissue, in particular to a fat graft enriched with highly active fat granule cells and adipose stem cells, and a preparation method and application thereof. Background technique [0002] Autologous adipose tissue is regarded as an ideal soft tissue filling material because of its biocompatibility, stability, natural postoperative effect, easy access to materials, abundant sources, less damage to the donor site, and repeatable surgery. Clinically, the use of autologous fat grafting by plastic surgeons is on the rise. [0003] The survival of transplanted adipose tissue is closely related to the reconstruction of blood vessels and nerves in adipose tissue. It has been confirmed that adipose tissue-derived adipose stem cells can stimulate angiogenesis, and can release angiogenic factors under the condition of ischemia and hypoxia after adipose t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/50
CPCA61L27/3804A61L27/3834A61L27/3895A61L27/50A61L2430/40
Inventor 杨远袁祥徐子林潘红伟韩飞陈俊杰叶飞轮
Owner CHENGDU YUANSHAN BIOTECH CO LTD
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