Process for improving extraction rate of cannabidiol by treating flowers and leaves of Cannabis sativa by using microorganisms

A technology of microbial treatment and cannabidiol, which is applied in the preparation of organic compounds, bulk chemical production, chemical instruments and methods, etc., can solve the problems of limited transformation, achieve improved extraction rate, shorten extraction time, and increase economic benefits Effect

Inactive Publication Date: 2019-07-23
YUNNAN LVXIN BIOLOGICAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this transformation is limited

Method used

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  • Process for improving extraction rate of cannabidiol by treating flowers and leaves of Cannabis sativa by using microorganisms
  • Process for improving extraction rate of cannabidiol by treating flowers and leaves of Cannabis sativa by using microorganisms
  • Process for improving extraction rate of cannabidiol by treating flowers and leaves of Cannabis sativa by using microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Pretreatment: Take the leaves, bark and root tissue of fresh cannabis plants that are growing well, and wash the stains on the surface with water. Wipe with 50% ethanol-water solution to kill bacteria on the tissue surface. Afterwards, it was washed with first-grade sterile water and dried in a sterile room for later use.

[0020] Be careful not to mix with diseased plant tissues, avoid bringing in other bad bacteria, and affect the culture of bacteria. Wipe with 50% ethanol solution, the surface of the tissue should be wet, and the tissue should not be soaked in the ethanol solution to prevent ethanol from penetrating into the tissue cells and killing intracellular fungi.

[0021] 2) Strain culture: Take the surface sterile and dry cannabis plant tissue, place it in a PDA plate medium in a sterile room, and culture it aseptically for 48 hours. Pick the white mycelium from the plate on the aseptic operating table, put it into the inclined surface of the test tube, ...

Embodiment 2

[0027] 1) Pretreatment: Take the leaves, bark and root tissue of a well-growing fresh cannabis plant, and wash the stains on the surface with water. Wipe with 50% ethanol-water solution to kill bacteria on the tissue surface. Afterwards, it was washed with first-grade sterile water and dried in a sterile room for later use. Be careful not to mix with diseased plant tissues, avoid bringing in other bad bacteria, and affect the culture of bacteria. Wipe with 50% ethanol solution, the surface of the tissue should be wet, and the tissue should not be soaked in the ethanol solution to prevent ethanol from penetrating into the tissue cells and killing intracellular fungi.

[0028] 2) Strain culture: Take the surface sterile and dry cannabis plant tissue, place it in a PDA plate medium in a sterile room, and culture it aseptically for 48 hours. Pick the white mycelium from the plate on the aseptic operating table, put it into the inclined surface of the test tube, and continue to c...

Embodiment 3

[0034]1) Pretreatment: Take the leaves, bark and root tissue of a well-growing fresh cannabis plant, and wash the stains on the surface with water. Wipe with 50% ethanol-water solution to kill bacteria on the tissue surface. Afterwards, it was washed with first-grade sterile water and dried in a sterile room for later use.

[0035] Be careful not to mix with diseased plant tissues, avoid bringing in other bad bacteria, and affect the culture of bacteria. Wipe with 50% ethanol solution, the surface of the tissue should be wet, and the tissue should not be soaked in the ethanol solution to prevent ethanol from penetrating into the tissue cells and killing intracellular fungi.

[0036] 2) Strain culture: Take the surface sterile and dry cannabis plant tissue, place it in a PDA plate medium in a sterile room, and culture it aseptically for 48 hours. Pick the white mycelium from the plate on the aseptic operating table, put it into the inclined surface of the test tube, and conti...

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Abstract

The invention belongs to the field of plant extraction, and particularly relates to a method for treating industrial flowers and leaves of Cannabis sativa by using microorganisms and improving the extraction rate of cannabidiol. The method comprises the following steps: 1) separating one or more fungi by using the industrial Cannabis sativa plants, and culturing and propagating the fungi, then fermenting the industrial flowers and leaves of Cannabis sativa by using the fungi to effectively destroy cell walls, so that the cell permeability of the flowers and leaves of Cannabis sativa is improved; 2) extracting and filtering the fermented flowers and leaves of Cannabis sativa by using organic solvents such as ethanol and n-hexane, and concentrating the obtained filtrate under reduced pressure in vacuum to obtain crude extract containing cannabidiol; 3) separating and purifying the cannabidiol from the extract by silica gel column chromatography; and 4) further purifying cannabidiol through crystallization to obtain the high-purity CBD crystal with the liquid phase content of 99.2%. According to the method, the enzymatic reaction of microorganisms is fully utilized to destroy plant cell walls, the extraction efficiency of a target object reaches 110-130%, the production cost is effectively reduced, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to a technique for improving the extraction rate of cannabidiol by using microorganisms to treat industrial hemp flowers and leaves. Background technique [0002] Industrial hemp refers to cannabis with a tetrahydrocannabinol content of less than 0.3%. In my country, industrial hemp is called hemp, which is a 1-year-old herb of the genus Cannabis in the family Cannabinaceae. At present, there are more than 100 kinds of cannabinoids (CBs) isolated from the plant cannabis, mainly including tetrahydrocannabinol (tetrahydrocannabinol, THC), cannabidiol (cannabidiol, CBD) and cannabinol (cannabinol, CBN). and cannabichromene (CBC), among which THC and CBD have the highest content. Tetrahydrocannabinol (THC) has hallucinogenic and addictive properties, which limits the development of its medicinal value. Cannabidiol (CBD) is completely different from tetrahydrocannabinol (THC)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C37/70C07C37/84C07C37/82C07C39/23
CPCC07C37/004C07C37/82C07C37/84C07C2601/16C07C39/23Y02P20/54
Inventor 王钲霖刘胜贵李智高付彬彬孔令羽
Owner YUNNAN LVXIN BIOLOGICAL PHARMA CO LTD
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