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Positive serum for riemerella anatipestifer (RA) as well as preparation method and application of positive serum

A duck plague Rimmer's, positive serum technology, applied in chemical instruments and methods, from serum immunoglobulin, immunoglobulin, etc., can solve problems such as high morbidity and mortality, lack of immune protection, and difficulty in prevention and control work , to achieve the effect of good application prospects

Active Publication Date: 2019-07-23
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease infects a wide range of hosts, high morbidity and mortality, easy to produce drug resistance, and there are many serotypes, lack of immune protection between different serotypes, which brings great difficulties to the prevention and treatment of the disease. The duck industry has caused huge economic losses

Method used

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  • Positive serum for riemerella anatipestifer (RA) as well as preparation method and application of positive serum
  • Positive serum for riemerella anatipestifer (RA) as well as preparation method and application of positive serum
  • Positive serum for riemerella anatipestifer (RA) as well as preparation method and application of positive serum

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Experimental program
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Effect test

Embodiment 1

[0020] (1) Preparation of immunogen

[0021] The recombinant protein OmpA of Riemerella anatipestifer preserved in the laboratory was used as the immunogen, and the protein concentration was 1.5 mg / mL, which was mixed with Freund's complete adjuvant and Freund's incomplete adjuvant respectively according to the volume ratio of 1:1. , put the mixture into the syringe, push and pull back and forth between the two syringes connected by the three-way valve, and mix repeatedly until the mixture drops on the water surface and becomes spherical and does not disperse. and immunogen emulsified in Freund's incomplete adjuvant.

[0022] Riemerella anatipestifer RAHB-1 and / or Riemerella anatipestifer RAHB-2.

[0023] (2) Preparation of bacterial solution

[0024] Riemerella anatipestifer RAHB-1 (preservation number is CCTCC NO: M2018805, depository unit: China Center for Type Culture Collection, deposit address: China. Wuhan. Wuhan University) and Riemerella anatipestifer RAHB-2 ( The ...

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Abstract

The invention belongs to the technical field of biological products, and particularly relates to positive serum for riemerella anatipestifer (RA) as well as a preparation method and application of thepositive serum. According to the preparation method, 4 times of immunization are performed on each duck, firstly, 3 times of immunization are performed by use of recombinant protein of the RA, the last immunization is finally performed by use of viable bacteria in a bacterial solution of the RA, so that after ducks are immune to the recombinant protein of the RA to produce antibody protection, high RA antibodies are produced under the stimulation of viable bacteria. The titer of the prepared positive serum for the RA is 1:12800 or above; and the positive serum for the RA, prepared by the method provided by the invention provides good technical support for accurate diagnosis and immune monitoring of the RA disease and accurate evaluation on the vaccine immune effect on the RA disease, andhas bright application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a Riemerella anatipestifer positive serum and a preparation method and application thereof. Background technique [0002] Riemerella anatipestifer (RA) disease is an important bacterial disease of waterfowl. Infectious serositis in famous ducks, the main autopsy pathological changes are fibrinous pericarditis, perihepatitis, air sac inflammation, meningitis, caseous salpingitis and so on. The disease infects a wide range of hosts, high morbidity and mortality, easy to produce drug resistance, and there are many serotypes, lack of immune protection between different serotypes, which brings great difficulties to the prevention and treatment of the disease. The duck industry has caused huge economic losses. Outer membrane protein A (OmpA) of Riemerella anatipestifer is the surface antigen of the outer membrane protein of Gram-negative bacteria. It is a rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K16/06G01N33/569
CPCC07K16/1203C07K16/06G01N33/56911
Inventor 罗玲温国元罗青平邵华斌王红琳卢琴汪宏才张腾飞张蓉蓉商雨张文婷
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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