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Method for identifying and detecting seven crude drugs in hepatitis B detoxification capsule

A hepatitis B Jiedu capsule, identification and detection technology, applied in the directions of measuring device, material separation, analysis material, etc., can solve the problems of low detection efficiency, limited separation ability of thin-layer chromatography, etc., and achieve good specificity, low cost, and detection. good precision

Active Publication Date: 2019-07-23
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of limited separation ability and low detection efficiency of TLC in the above prior art, the present application provides a method that is easy to operate, good in separation effect, and high in detection efficiency while simultaneously identifying Phellodendron Phellodendron, Scutellaria baicalensis, rhubarb, Detection method of seven medicinal flavors of Quanshen, Huhuanglian, Guanzhong and Smilax smilax

Method used

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  • Method for identifying and detecting seven crude drugs in hepatitis B detoxification capsule
  • Method for identifying and detecting seven crude drugs in hepatitis B detoxification capsule
  • Method for identifying and detecting seven crude drugs in hepatitis B detoxification capsule

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Instruments and reagents

[0053] Instruments: Mettler Toledo XSE205 electronic balance; Waters 2695-2998 high-performance liquid chromatography (PDA detector); Thermofisher Q Exactive high-resolution mass spectrometer.

[0054] Control medicinal materials: all purchased from China National Institutes for Food and Drug Control, Cortex Phellodendron (batch number: 121510-201606), Scutellaria baicalensis reference medicinal material (batch number: 120955-201810), rhubarb reference medicinal material (batch number: 120984-201202), boxing ginseng reference medicinal material (batch number: 121569-201602), Huhuanglian reference medicinal material (batch number: 121073-201503), Guanzhong reference medicinal material (batch number: 121034-200302) and Smilax smilax reference medicinal material (batch number: 121439-201803).

[0055] Reagent: Methanol and acetonitrile are chromatographically pure, and phosphoric acid is analytically pure. Hepatitis B Jiedu Capsule is a comme...

Embodiment 2

[0078] The mensuration of embodiment 2 sample

[0079] Nationwide, there are currently eight Hepatitis B Jiedu Capsule manufacturers in production. This experiment selected samples from all eight companies for investigation. The results are shown in Table 2.

[0080] Table 2 Sample determination results of different enterprises

[0081]

[0082]

[0083] Note: "+" means that the chromatographic peak was detected, "-" means that the chromatographic peak was not detected

[0084] From the results in the table above, it can be seen that no peaks of No. 7 and No. 12, peaks of berberine and berberine were not detected in samples 2 and 5, indicating that there may be problems with the quality or feeding of berberine in the samples; all the characteristic peaks in other samples All were detected, indicating that the flavors of each medicine in the sample were basically normal. Figure 10 with Figure 11 The HPLC chromatograms of qualified sample 1 and unqualified sample 2 ar...

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Abstract

The invention relates to the technical field of drug detection, in particular to a method for identifying and detecting seven crude drugs in a hepatitis B detoxification capsule. Taking a sample of the hepatitis B detoxification capsule to be tested; extracting with an organic solvent to obtain a test solution; and injecting into a high performance liquid chromatograph for detection, so that the seven crude drugs of Phellodendron amurense, Scutellaria baicalensis, Rhubarb, Polygonum bistorta, Rhizoma picrorhizae, Cyrtomium fortunei and Rhizoma smilacis glabrae in the hepatitis B detoxificationcapsule can be detected simultaneously. The method for identifying and detecting the seven crude drugs in the hepatitis B detoxification capsule is simple, easy to operate, has short analysis time, low cost and good reproducibility, and can be used for quality control of the hepatitis B detoxification capsule; the method has better specificity; and the RSD of the relative retention time of the 20characteristic peaks is less than 2%, and the detection precision is good.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for identifying and detecting seven kinds of raw materials in Hepatitis B Jiedu Capsules. Background technique [0002] Hepatitis B Jiedu Capsules are composed of Phellodendron Phellodendron, Scutellaria Baicalensis, Rhubarb, Quanshen, Huhuanglian, Guanzhong, Smilax Smilax and Saponin. The current quality standard is simple. Except for the microscopic identification of Smilax Smilax, there is no other difference with the eight herbs. related testing items. After reviewing the literature, there are only thin-layer identification methods for the three medicinal flavors of Phellodendron Phellodendron, Scutellaria baicalensis, and rhubarb, but no method for simultaneous identification of multiple medicinal flavors in Bganjiedu Capsules by using high performance liquid chromatography. [0003] Thin-layer chromatography is a widely used qualitative identification metho...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 谭会洁林林林永强于凤蕊刘洪超李军
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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