Method for preparing Pixian thick broad-bean sauce rich in gamma-aminobutyric acid
A technology of aminobutyric acid and production methods, applied in the direction of bacteria used in food preparation, food science, applications, etc., to achieve the effect of filling market gaps, enhancing nutritional and health value, and improving nutritional and health characteristics
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Embodiment 1
[0044] A preparation method of bean paste rich in gamma-aminobutyric acid, comprising the following operations:
[0045] 1) Preparation of Lactobacillus plantarum BC114 fermentation broth: activate Lactobacillus plantarum BC114 producing γ-aminobutyric acid in MRS medium containing 5% (m / v) L-sodium glutamate, and cultivate to logarithmic During the growth period, the bacterial concentration was 10 7 CFU / mL.
[0046]2) Preparation of fermentation broth of Saccharomyces cerevisiae JM037: activate Saccharomyces cerevisiae JM037 producing γ-aminobutyric acid in YPD medium containing 5% (m / v) L-sodium glutamate, and culture to logarithmic growth phase , the bacterial solution concentration is 10 6 CFU / mL.
[0047] 3) Screen the broad beans in turn, shell them, soak them in hot water, mix them with wheat flour (4:1, m / m), inoculate them with Aspergillus oryzae (Shanghai Niang 3.042) bacteria powder to make koji, the inoculation amount of the bacteria powder is 1%; natural ventil...
Embodiment 2
[0052] A preparation method of bean paste rich in gamma-aminobutyric acid, comprising the following operations:
[0053] 1) Preparation of Lactobacillus plantarum BC114 fermentation broth: activate Lactobacillus plantarum BC114 producing γ-aminobutyric acid in MRS medium containing 5% (m / v) L-sodium glutamate, and cultivate to logarithmic During the growth period, the bacterial concentration was 10 7 CFU / mL.
[0054] 2) Preparation of fermentation broth of Saccharomyces cerevisiae JM037: activate Saccharomyces cerevisiae JM037 producing γ-aminobutyric acid in YPD medium containing 5% (m / v) L-sodium glutamate, and culture to logarithmic growth phase , the bacterial solution concentration is 10 6 CFU / mL.
[0055] 3) Screen the broad beans in turn, shell them, soak them in hot water, mix them with wheat flour (3:1, m / m), inoculate them with Aspergillus oryzae (Shanghai Niang 3.042) bacteria powder to make koji, the inoculation amount of the bacteria powder is 1.5%; natural ven...
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