A kind of preparation method and application of mhpbs-hemin/rgo labeled electrochemical immunosensor
An immunosensor, electrochemical technology, applied in the direction of chemical instruments and methods, electrochemical variables of materials, nanotechnology for sensing, etc., can solve the problems of cumbersome sample pretreatment, long detection cycle, unsatisfactory, etc., and achieve relief Effects of irreversible stacking phenomenon, reduction of aggregation and deactivation, and improvement of sensitivity
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Embodiment 1
[0042] Example 1 A method of preparing an electrochemical immunosensor of MHPBS-HEMIN / RGO, steps are as follows:
[0043] (1) Polishing a glass carbon electrode having a diameter of 4.0 mm into a mirror surface, and clean it in ultrapure water;
[0044] (2) Take 6.0 μL of MUA-Au NPS to the surface of the electrode, dry at room temperature, clean the surface of the electrode with ultrapure water, dry;
[0045] (3) Continue to capture antibody ABs from 6.0 μL, 10.0 μg / ml of yellowlaxin 1 Add to the surface of the electrode, place it in a 4.0 ° C refrigerator;
[0046] (4) 3.0 μL, 0.5% of the bovine serum protein BSA solution is added to the surface of the electrode to enclose the non-specific active site on the surface of the electrode, and the electrode surface is cleaned with phosphate buffer with pH = 7.2, placed in 4.0 °. Ceiling in the C refrigerator;
[0047] (5) A series of different concentrations of a series of different concentrations of a series of xeroxin antigenic s...
Embodiment 2
[0049] Example 2 A method of preparing an electrochemical immunosensor of MHPBS-HEMIN / RGO, steps are as follows:
[0050] (1) Polishing a glass carbon electrode having a diameter of 4.0 mm into a mirror surface, and clean it in ultrapure water;
[0051] (2) Take 6.0 μL of MUA-Au NPS to the surface of the electrode, dry at room temperature, clean the surface of the electrode with ultrapure water, dry;
[0052] (3) Continue to capture antibody ABs from 6.0 μL, 15.0 μg / ml 1 Add to the surface of the electrode, place it in a 4.0 ° C refrigerator;
[0053] (4) 3.0 μL, 1.0% of the bovine serum protein BSA solution is added to the surface of the electrode to enclose the non-specific active site on the surface of the electrode, and the electrode surface is cleaned with phosphate buffer with pH = 7.2, placed in 4.0 °. Ceiling in the C refrigerator;
[0054] (5) Add 6.0 μL, 1.0 pg / ml ~ 100.0 ng / ml of a series of different concentrations of yellowlaxin antigenic solution, after 35.0 ...
Embodiment 3
[0056] Example 3 A method of preparing an electrochemical immunosensor of MHPBS-HEMIN / RGO, steps are as follows:
[0057] (1) Polishing a glass carbon electrode having a diameter of 4.0 mm into a mirror surface, and clean it in ultrapure water;
[0058] (2) Take 6.0 μL of MUA-Au NPS to the surface of the electrode, dry at room temperature, clean the surface of the electrode with ultrapure water, dry;
[0059] (3) Continue to capture antibody ABs from 6.0 μL, 20.0 μg / ml of yellowlaxin 1 Add to the surface of the electrode, place it in a 4.0 ° C refrigerator;
[0060] (4) 3.0 μL, 1.5% bovine serum protein BSA solution is added to the surface of the electrode, and the non-specific active site on the surface of the electrode is to be cleaned with pH = 7.2 phosphate buffer, placed in 4.0 °. Ceiling in the C refrigerator;
[0061] (5) Add 6.0 μL, 1.0 pg / ml ~ 100.0 ng / ml of a series of different concentrations of yellowlaxin antigen solution, 40.0 min in a 4.0 ° C refrigerator, w...
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