Silkworm egg combination suitable for producing medicinal bombyx batryticatus
A technology for silkworm seeds and dead silkworms, applied in animal husbandry and other directions, can solve the problems that the yield and quality of dead silkworms cannot be guaranteed, the yield and quality of dead silkworms are affected, and the sensitivity of Beauveria bassiana is different, so as to achieve stable yield and improve inoculation. The success rate and the effect of strong operability
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Embodiment 1
[0017] Embodiment 1 Screening of Bombyx mori silkworm eggs
[0018] 1 Experimental materials
[0019] 1.1 Source of materials
[0020] 7532×932, Jinyuan×Lingzhou, Baichun×Fangxiu, Chuanshan×Shushui, Liangguang No. 2 silkworm eggs (purchased from Langzhong Silkworm Farm in Sichuan Province), Beauveria assiana (Bals.) Vuillant provided by Chengdu University of Traditional Chinese Medicine).
[0021] 1.2 Instruments and reagents
[0022] Agilent 1200 HPLC High Performance Liquid Chromatography (Agilent, USA); KQ-500DE CNC Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); DZKW-4 Electronic Constant Temperature Water Bath (Beijing Zhongxing Weiye Instrument Co., Ltd.); Constant Temperature Drum Air drying oven (Shanghai Langgan Experimental Equipment Co., Ltd.); BP-121s precision electronic balance (one hundred thousandth, Zhejiang Precision Instrument Co., Ltd.); BS-200S-WEI precision electronic balance (one thousandth, Beijing Sartorius Instrument Systems Ltd); mu...
Embodiment 2
[0057] Embodiment 2 Bombyx mori culture method
[0058] 1 Experimental materials
[0059] Silkworm eggs from Jinyuan×Lingzhou, Baichun×Fangxiu, Chuanshan×Shushui, Liangguang No. supply).
[0060] 2 Experimental design
[0061] 2.1 Inoculation method
[0062] Prepare the stock solution of Beauveria bassiana with sterile water containing 0.05% Tween 80 (23-28°C), measure the concentration of spores with a hemocytometer, and dilute the stock solution of Beauveria bassiana to 1×10 8 piece / mm 2 . Select mulberry-eating vigorous fifth-instar silkworms, inoculate them with the working solution of Beauveria bassiana, and spray them 3 times until the silkworms see dewdrops. Keep the temperature at 25±2°C and the humidity at 90±5%RH, and feed the mulberry leaves after 2 hours of control.
[0063] 2.2 Breeding method
[0064] The experimental verification takes silkworm breeding as the control, screens silkworm eggs in spring and late autumn, and screens silkworm eggs in summer a...
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