Silkworm egg combination suitable for producing medicinal bombyx batryticatus

A technology for silkworm seeds and dead silkworms, applied in animal husbandry and other directions, can solve the problems that the yield and quality of dead silkworms cannot be guaranteed, the yield and quality of dead silkworms are affected, and the sensitivity of Beauveria bassiana is different, so as to achieve stable yield and improve inoculation. The success rate and the effect of strong operability

Active Publication Date: 2019-07-30
SICHUAN GUANGYUAN MINJIANG CHINESE HERBAL MEDICINE PLANTING CO LTD
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AI Technical Summary

Problems solved by technology

Different silkworm species have different sensitivities to Beauveria bassiana, resulting in different growth and development after inoculation, which ultimately affects the yield and quality of silkworms
[0004] Yang Qiong et al., Research on the Artificial Culture of Medicinal Bombyx larvae [J], Guangdong Sericulture 44(4): 34-38 discloses the artificial culture method of Bombyx botany, strain screening, optimal inoculation dose, suitable silkworm Among them, the silkworm eggs used are...

Method used

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  • Silkworm egg combination suitable for producing medicinal bombyx batryticatus
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  • Silkworm egg combination suitable for producing medicinal bombyx batryticatus

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Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1 Screening of Bombyx mori silkworm eggs

[0018] 1 Experimental materials

[0019] 1.1 Source of materials

[0020] 7532×932, Jinyuan×Lingzhou, Baichun×Fangxiu, Chuanshan×Shushui, Liangguang No. 2 silkworm eggs (purchased from Langzhong Silkworm Farm in Sichuan Province), Beauveria assiana (Bals.) Vuillant provided by Chengdu University of Traditional Chinese Medicine).

[0021] 1.2 Instruments and reagents

[0022] Agilent 1200 HPLC High Performance Liquid Chromatography (Agilent, USA); KQ-500DE CNC Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); DZKW-4 Electronic Constant Temperature Water Bath (Beijing Zhongxing Weiye Instrument Co., Ltd.); Constant Temperature Drum Air drying oven (Shanghai Langgan Experimental Equipment Co., Ltd.); BP-121s precision electronic balance (one hundred thousandth, Zhejiang Precision Instrument Co., Ltd.); BS-200S-WEI precision electronic balance (one thousandth, Beijing Sartorius Instrument Systems Ltd); mu...

Embodiment 2

[0057] Embodiment 2 Bombyx mori culture method

[0058] 1 Experimental materials

[0059] Silkworm eggs from Jinyuan×Lingzhou, Baichun×Fangxiu, Chuanshan×Shushui, Liangguang No. supply).

[0060] 2 Experimental design

[0061] 2.1 Inoculation method

[0062] Prepare the stock solution of Beauveria bassiana with sterile water containing 0.05% Tween 80 (23-28°C), measure the concentration of spores with a hemocytometer, and dilute the stock solution of Beauveria bassiana to 1×10 8 piece / mm 2 . Select mulberry-eating vigorous fifth-instar silkworms, inoculate them with the working solution of Beauveria bassiana, and spray them 3 times until the silkworms see dewdrops. Keep the temperature at 25±2°C and the humidity at 90±5%RH, and feed the mulberry leaves after 2 hours of control.

[0063] 2.2 Breeding method

[0064] The experimental verification takes silkworm breeding as the control, screens silkworm eggs in spring and late autumn, and screens silkworm eggs in summer a...

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Abstract

The invention provides a silkworm egg combination suitable for producing medicinal bombyx batryticatus. The silkworm egg combination comprises the following varieties of silkworm eggs: Baichun and Fangxiu, Chuanshan and Shushui, Jinyuan and Lingzhou, and Liangguang No.2. The invention also provides a medicinal bombyx batryticatus breeding method which comprises the following steps: alternately breeding Baichun and Fangxiu or/and Chuanshan and shushui in spring and late autumn, and selecting Jinyuan and Lingzhou or Liangguang No. 2 in summer and early autumn, wherein the breeding temperature is26+/-2 DEG C, and the humidity is 75+/-5% RH; and enabling the silkworm eggs to be satiated fully. The silkworm egg combination has the advantages of high operability, cost saving, relatively high yield of produced bombyx batryticatus, relatively good appearance and good quality, and the method is suitable for artificially large-scale breeding of bombyx batryticatus.

Description

technical field [0001] The invention relates to a combination of silkworm eggs produced by multi-season medicinal silkworms. Background technique [0002] Bombyx mori is derived from the dead body of Bombyx mori Linnaeus 4-5 instar larvae infected with Beauveria assiana (Bals.) Vuillant. , convulsions, epilepsy, convulsions in children, tetanus and other diseases. With the in-depth research on the pharmacological components and functions of the silkworm and the continuous development of related new products, the application of the silkworm in the medicinal field will become more and more extensive. In the actual production process of Bombyx mori, different silkworm species show different infection rates to Beauveria bassiana and other miscellaneous bacteria, resulting in different growth cycles of Bombyx mori, unstable yield, and ultimately affecting the quality of Bombyx mori. [0003] Since ancient times, silkworm eggs are mainly derived from silkworm species. Silkworms ...

Claims

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Application Information

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IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 何刚李永川肖禾刘文武
Owner SICHUAN GUANGYUAN MINJIANG CHINESE HERBAL MEDICINE PLANTING CO LTD
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