A kind of method and its application of preparing chrysanthemum alkyne a from Huai chrysanthemum
A technology of chrysanthemum and new alkyne, which is applied in the field of medicine, can solve the problem of reduction, achieve easy operation, significant economic and social benefits, and improve the effect of medicinal value and commercial value
Active Publication Date: 2022-03-29
HENAN UNIV OF CHINESE MEDICINE
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Experimental results show that chrysanthemum A can significantly reduce LPS-induced NO release from RAW264.7 cells, and has a good anti-inflammatory effect, but there has been no public report so far
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[0019] The specific embodiments of the present invention will be described in detail below in conjunction with specific circumstances.
[0020] In the specific implementation of the present invention, a method for preparing chrysanthemum new alkyne A from Huai chrysanthemum, said chrysanthemum new alkyne A is an active ingredient extracted from Huai chrysanthemum, and its preparation method is: Huai chrysanthemum 11.2kg, using volume The acetone aqueous solution with a concentration of 50% was broken and extracted twice. The amount of acetone was 120L each time. The two extracts were combined and concentrated under reduced pressure to obtain 1.8kg of extract; the extract was dispersed with 9L of water, followed by 5×10L of petroleum ether, ethyl acetate 5×10L of ester and 5×10L of n-butanol were extracted to obtain petroleum ether part, ethyl acetate part, n-butanol part and water part; , 30:1, 20:1, 5:1 for gradient elution to obtain the corresponding four fractions Fr.1~4; ...
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The present invention relates to a method for preparing chrysanthemum A from Huai chrysanthemum and its application, which can effectively solve the problem of extracting chrysanthemum A from Huai chrysanthemum and realize the application of chrysanthemum A as the only active ingredient in the preparation of anti-inflammatory drugs. Chrysanthemum chrysanthemum was extracted by crushing and extracting with acetone aqueous solution, the extract was concentrated under reduced pressure to form an extract, the extract was dispersed in water, extracted with petroleum ether, ethyl acetate, and n-butanol in sequence, the ethyl acetate part was placed on a silica gel column, and dichloromethane- Gradient elution with methanol yielded fractions Fr.1~4; fraction Fr.3 was dissolved in methanol, centrifuged, and filtered to remove insoluble matter, and the obtained sub-fraction Fr.3.1 was concentrated under reduced pressure, put on an MCI column, and washed with water and methanol in sequence. Gradient elution, spray detection with anisaldehyde-concentrated sulfuric acid, the obtained sub-fraction Fr.3.1.1 was separated by semi-preparative HPLC, collected at retention time t R =25.0~27.0min fractions were concentrated and dried to obtain the compound chrysanthene A. The preparation method of the invention is easy to operate, scientific and reasonable, opens up a new way of anti-inflammatory drugs, and improves the medical value and commercial value of Huai chrysanthemum.
Description
technical field [0001] The invention relates to medicine, in particular to a method for preparing chrysanthemum new alkyne A from Huai chrysanthemum and its application. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium Ramat.) is the dried flower head of chrysanthemum of the Compositae family. Its taste is sweet, bitter, and cool in nature. It mainly belongs to the lung and liver meridians. The 2015 edition of the Chinese Pharmacopoeia added the species "Huai chrysanthemum", which is used as "chrysanthemum" together with "Boju", "Chu Ju", "Gong Ju" and "Hang Ju", affirming the medicinal use of Huai chrysanthemum status. Huai chrysanthemum [Dendranthema morifolium (Ramat.) kitam], as one of the "Four Huai Medicines", is mainly produced in Wuzhi and Wenxian areas in Henan Province. It has a long history of planting and is one of the authentic medicinal materials in Henan. Huai chrysanthemum has the functions of clearing away heat and detoxification, dis...
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IPC IPC(8): C07H15/04C07H1/08A61P29/00A61K31/7028
CPCC07H15/04C07H1/08A61P29/00
Inventor 李孟曾梦楠张靖柯石静亚吕锦锦陈颖婕王洋洋郑晓珂冯卫生
Owner HENAN UNIV OF CHINESE MEDICINE