A kind of cassia twig polyphenol with the activity of inhibiting the proliferation of intestinal cancer cells and its preparation method and application

A technology for colon cancer cells and cinnamon sticks is applied in the field of cinnamon stick polyphenols and their preparation, which can solve the problems of complex chemical composition, difficult to clarify, and the mechanism of action has not yet been elucidated, and achieves the effect of inhibiting colon cancer cells with simple steps.

Active Publication Date: 2021-10-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, cassia twig is mainly in the form of unpurified crude water extract compatible with other traditional Chinese medicines in clinical application. The chemical composition is extremely complex, and it is difficult to determine whether it contains other ingredients that can cause side effects in addition to the active ingredients, and the specific The mechanism of action has not been elucidated

Method used

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  • A kind of cassia twig polyphenol with the activity of inhibiting the proliferation of intestinal cancer cells and its preparation method and application
  • A kind of cassia twig polyphenol with the activity of inhibiting the proliferation of intestinal cancer cells and its preparation method and application
  • A kind of cassia twig polyphenol with the activity of inhibiting the proliferation of intestinal cancer cells and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of cassia twig polyphenols with the activity of inhibiting the proliferation of intestinal cancer cells, comprising the steps of:

[0033] Use a pulverizer to make cassia twig raw materials into powder, pass through a 20-mesh sieve, add 2000 mL of ethanol solution with a volume fraction of 95% according to the ratio of material to liquid of 50:1 mg / mL, and extract in a water bath at 80 ° C for 1 hour, and extract twice; The alcohol extract is put into a centrifuge and centrifuged for 10min under the condition of 5000r / min to remove the solid residue; the crude extract is concentrated under reduced pressure and vacuum and freeze-dried in vacuum to obtain the cinnamon twig freeze-dried powder (that is, the untreated cinnamon used in the test of the following example 5). Resin-treated cassia twig extract); the cassia twig freeze-dried powder was redissolved with deionized water to be prepared into a 25mg / mL cassia twig powder solution, and added 2g of t...

Embodiment 2

[0035] A preparation method of cassia twig polyphenols with the activity of inhibiting the proliferation of intestinal cancer cells, comprising the steps of:

[0036]Use a pulverizer to make raw materials of cassia twig into powder, pass through a 60-mesh sieve, add 1000 mL of absolute ethanol according to the ratio of 100:1 mg / mL to liquid, and extract in a water bath at 75 ° C for 2 hours; put the alcohol extract in a centrifuge Centrifuge at 4000r / min for 15min to remove solid residues; the crude extract is vacuum-concentrated under reduced pressure and then vacuum freeze-dried; reconstitute the cinnamon twig freeze-dried powder with deionized water to prepare a 25mg / mL solution, add 2g The proportion of the activated AB-8 macroporous resin is statically adsorbed, and adsorbed in a shaking table at 25°C and 100rpm for 8h; the adsorbed macroporous resin is filtered to remove the liquid, and 20mL of ethanol with a volume fraction of 95% is added The solution was desorbed in a...

Embodiment 3

[0038] A preparation method of cassia twig polyphenols with the activity of inhibiting the proliferation of intestinal cancer cells, comprising the steps of:

[0039] Use a pulverizer to make cassia twig raw materials into powder, pass through a 40-mesh sieve, add 1250 mL of ethanol solution with a volume fraction of 90% ethanol according to the ratio of material to liquid of 80:1 mg / mL, and extract in a water bath at 80 °C for 1.5 h; The extract was placed in a centrifuge and centrifuged at 6000r / min for 12min to remove solid residues; the crude extract was concentrated under reduced pressure and then vacuum freeze-dried; the lyophilized cassia twig powder was reconstituted with deionized water to make a 25mg / mL solution , according to the ratio of 20mL Guizhi solution plus 2g activated D101 macroporous resin, perform static adsorption, and adsorb in a shaking table at 25°C and 50rpm for 10h; filter the adsorbed macroporous resin to remove the liquid, and add 20mL The volume ...

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Abstract

The invention discloses a cassia twig polyphenol with the activity of inhibiting the proliferation of intestinal cancer cells, a preparation method and application thereof. The method comprises: pulverizing and sieving cassia twigs to obtain cassia twig powder, adding the cassia twig powder into ethanol solution, mixing evenly, and heating for extraction to obtain an extract; centrifuging the extract to obtain a supernatant, filtering to obtain a filtrate , concentrated, freeze-dried to obtain freeze-dried powder; add the freeze-dried powder into water, mix well to obtain a mixed solution, add macroporous resin to the mixed solution, carry out adsorption treatment, take filter residue by suction filtration, add filter residue to ethanol solution, and carry out desorption treatment to obtain a desorption solution; the desorption solution is suction-filtered, the filtrate is taken, concentrated, and freeze-dried to obtain the cinnamon twig polyphenols that can inhibit the proliferation of intestinal cancer cells. The cassia twig extract provided by the invention has the activity of inhibiting the proliferation of human colon cancer cells SW620, can be used for the development of drugs for the preparation of colon cancer, and provides an experimental basis and scientific basis for the application of traditional Chinese medicine compatibility in clinical anti-tumor drug research.

Description

technical field [0001] The invention belongs to the technical field of cassia twig extraction, and in particular relates to a cassia twig polyphenol capable of inhibiting proliferation of intestinal cancer cells, a preparation method and application thereof. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive tract, mainly including colon cancer and rectal cancer. In recent years, the incidence and mortality have increased year by year. According to ACS "Cancer Statistics 2015", the mortality rate is second only to lung cancer, about 47%. The incidence and mortality of colon cancer in my country rank fifth among all malignant tumors, with 376,000 new cases and 191,000 deaths; the incidence and mortality increase rapidly after the age of 35, reaching a peak after the age of 80 ; The urban area is much higher than the rural area, and it is already in the middle and late stage when it is discovered, which seriously threatens the health an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/54A61P35/00
CPCA61K36/54A61K2236/333A61K2236/51A61K2236/55A61P35/00
Inventor 任娇艳高立
Owner SOUTH CHINA UNIV OF TECH
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