A kind of cassia twig polyphenol with the activity of inhibiting the proliferation of intestinal cancer cells and its preparation method and application
A technology for colon cancer cells and cinnamon sticks is applied in the field of cinnamon stick polyphenols and their preparation, which can solve the problems of complex chemical composition, difficult to clarify, and the mechanism of action has not yet been elucidated, and achieves the effect of inhibiting colon cancer cells with simple steps.
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Embodiment 1
[0032] A preparation method of cassia twig polyphenols with the activity of inhibiting the proliferation of intestinal cancer cells, comprising the steps of:
[0033] Use a pulverizer to make cassia twig raw materials into powder, pass through a 20-mesh sieve, add 2000 mL of ethanol solution with a volume fraction of 95% according to the ratio of material to liquid of 50:1 mg / mL, and extract in a water bath at 80 ° C for 1 hour, and extract twice; The alcohol extract is put into a centrifuge and centrifuged for 10min under the condition of 5000r / min to remove the solid residue; the crude extract is concentrated under reduced pressure and vacuum and freeze-dried in vacuum to obtain the cinnamon twig freeze-dried powder (that is, the untreated cinnamon used in the test of the following example 5). Resin-treated cassia twig extract); the cassia twig freeze-dried powder was redissolved with deionized water to be prepared into a 25mg / mL cassia twig powder solution, and added 2g of t...
Embodiment 2
[0035] A preparation method of cassia twig polyphenols with the activity of inhibiting the proliferation of intestinal cancer cells, comprising the steps of:
[0036]Use a pulverizer to make raw materials of cassia twig into powder, pass through a 60-mesh sieve, add 1000 mL of absolute ethanol according to the ratio of 100:1 mg / mL to liquid, and extract in a water bath at 75 ° C for 2 hours; put the alcohol extract in a centrifuge Centrifuge at 4000r / min for 15min to remove solid residues; the crude extract is vacuum-concentrated under reduced pressure and then vacuum freeze-dried; reconstitute the cinnamon twig freeze-dried powder with deionized water to prepare a 25mg / mL solution, add 2g The proportion of the activated AB-8 macroporous resin is statically adsorbed, and adsorbed in a shaking table at 25°C and 100rpm for 8h; the adsorbed macroporous resin is filtered to remove the liquid, and 20mL of ethanol with a volume fraction of 95% is added The solution was desorbed in a...
Embodiment 3
[0038] A preparation method of cassia twig polyphenols with the activity of inhibiting the proliferation of intestinal cancer cells, comprising the steps of:
[0039] Use a pulverizer to make cassia twig raw materials into powder, pass through a 40-mesh sieve, add 1250 mL of ethanol solution with a volume fraction of 90% ethanol according to the ratio of material to liquid of 80:1 mg / mL, and extract in a water bath at 80 °C for 1.5 h; The extract was placed in a centrifuge and centrifuged at 6000r / min for 12min to remove solid residues; the crude extract was concentrated under reduced pressure and then vacuum freeze-dried; the lyophilized cassia twig powder was reconstituted with deionized water to make a 25mg / mL solution , according to the ratio of 20mL Guizhi solution plus 2g activated D101 macroporous resin, perform static adsorption, and adsorb in a shaking table at 25°C and 50rpm for 10h; filter the adsorbed macroporous resin to remove the liquid, and add 20mL The volume ...
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