Preparation method of instant umami mushroom soup powder
A mushroom soup and instant technology, applied in the field of edible mushrooms, to achieve the effect of rich nutrition, short enzymatic hydrolysis time and sufficient release
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Embodiment 1
[0032] A kind of fresh instant bacteria soup powder
[0033] (1) Preparation of shiitake mushroom stock solution: crush dried shiitake mushrooms through a 40-mesh sieve, add 1g of dried shiitake mushroom powder to 10ml of water to make a shiitake mushroom stock solution for later use;
[0034] (2) Enzymolysis: adjust the pH of the mushroom stock solution to 5.5 with hydrochloric acid at a concentration of 1.00mol / L, add 0.45% of cellulase and 0.25% of flavor protease for enzymolysis, and the enzymolysis temperature is 52°C. Enzymolysis for 82 minutes to obtain enzymolysis solution;
[0035] (3) Enzyme inactivation centrifugation: heat the enzymolysis solution at 92.5°C for 12.5 minutes to inactivate the enzyme, then centrifuge at 4500r / min for 12.5 minutes, and take the supernatant for later use;
[0036] (4) High-temperature reaction: Mix the supernatant with xylose, glucose, and L-cysteine at a mass ratio of 100:5:6.5:2.5, adjust the pH to 7.8, and react at 102°C for 80 m...
Embodiment 2
[0042] A kind of fresh instant bacteria soup powder
[0043] (1) Preparation of shiitake mushroom stock solution: crush dried shiitake mushrooms through a 40-mesh sieve, add 1g of dried shiitake mushroom powder to 10ml of water to make a shiitake mushroom stock solution for later use;
[0044] (2) Enzymolysis: Adjust the pH of the mushroom stock solution to 5.5 with hydrochloric acid at a concentration of 1.00 mol / L, add 0.3% of cellulase and 0.2% of flavor protease for enzymolysis, and the enzymatic hydrolysis temperature is 50°C. The enzymolysis time is 75min, and the enzymolysis solution is obtained;
[0045] (3) Enzyme inactivation centrifugation: heat the enzymolysis solution at 90°C for 10 minutes to inactivate the enzyme, then centrifuge at 4500r / min for 10 minutes, and take the supernatant for later use;
[0046] (4) High-temperature reaction: Mix the supernatant with xylose, glucose, and L-cysteine at a mass ratio of 100:4:5:2, adjust the pH to 7.8, and react at 10...
Embodiment 3
[0052] A kind of fresh instant bacteria soup powder
[0053] (1) Preparation of shiitake mushroom stock solution: crush dried shiitake mushrooms through a 40-mesh sieve, add 1g of dried shiitake mushroom powder to 10ml of water to make a shiitake mushroom stock solution for later use;
[0054] (2) Enzymolysis: Adjust the pH of the mushroom stock solution to 5.5 with hydrochloric acid at a concentration of 1.00 mol / L, add 0.6% of cellulase and 0.3% of flavor protease for enzymolysis, and the enzymatic hydrolysis temperature is 55°C. The enzymolysis time is 90min, and the enzymolysis solution is obtained;
[0055] (3) Enzyme inactivation centrifugation: heat the enzymolysis solution at 95°C for 15 minutes to inactivate the enzyme, then centrifuge at 4500r / min for 15 minutes, and take the supernatant for later use;
[0056] (4) High-temperature reaction: Mix the supernatant with xylose, glucose, and L-cysteine at a mass ratio of 100:6:8:3, adjust the pH to 7.8, and react at 10...
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