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Rice DNA sample extraction method suitable for high-throughput genotyping

A technology of genotyping and extraction methods, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, microbial measurement/inspection, etc., can solve laborious processes, high costs, and complicated operation steps problem, to achieve the effect of overcoming the huge workload

Inactive Publication Date: 2019-08-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods usually have many and complicated steps, and the process is laborious, time-consuming, and costly, which have become the key factors that limit the efficiency and cost reduction in the process of large-scale rice mutant library construction.

Method used

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  • Rice DNA sample extraction method suitable for high-throughput genotyping
  • Rice DNA sample extraction method suitable for high-throughput genotyping
  • Rice DNA sample extraction method suitable for high-throughput genotyping

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention will be further described below in conjunction with specific embodiment 1 and accompanying drawings.

[0023] Materials and Reagents

[0024] 1.1 Experimental materials

[0025] Rice seeds, 96-well deep-well plate, 96-well PCR plate, metal ball (d=0.3cm), elastic skin, metal tray (32cm×22cm×3cm), microplate metal thermal block (QBLock-0.2), Qualcomm Quantitative tissue grinder (2010Genogrinder), glass rod (d=0.35cm), automatic micro-dispensing system (Mantis), etc.

[0026] 1.2 Experimental reagents

[0027] Dry ice, ethanol (95% v / v), 2% CTAB extract, 500 mM TRIS (pH 8), 50 mM EDTA (pH = 9), 2.5% sodium hypochlorite solution, etc.

[0028] 2.2 Treatment of seeds and cultivation of seedlings

[0029] 2.2.1 Treatment of seeds

[0030] Soak the rice seeds in 70% ethanol for 1 min, soak in 2.5% sodium hypochlorite solution for 30 min, after disinfection treatment, soak in water for 1 day, and then wrap the seeds with a wet rag to accelerate germi...

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Abstract

The invention discloses a rice DNA sample extraction method suitable for high-throughput genotyping, and relates to a biological DNA sample extraction method. A double-layer growth plate is prepared by combining a 96 deep-hole plate and a 96-hole PCR plate, the double-layer growth plate is used for breeding rice seeds, root DNA samples of 96 groups of rice can be extracted at a time, and the defects that a traditional method is huge in workload, wastes time and labor and cannot achieve high-throughput DNA extraction are overcome. The method is highly feasible and is suitable for automation; the DNA extraction method is also applicable to other biological samples.

Description

technical field [0001] The invention relates to the technical field of extracting rice DNA samples, in particular to a method for extracting rice DNA samples suitable for high-throughput genotyping. Background technique [0002] For plant genotyping, it is generally necessary to screen from a large number of populations and at the same time obtain tissue samples from individual plants in a manner that does not kill the plants. In addition, each sample has a one-to-one correspondence with the number, so that the target plants can be planted and multiplied after accurate screening. At present, there is no high-throughput tissue sampling method for a single plant. The commonly used method is that technicians take leaves or other tissues and organs of plants and number them, so that the taken plant samples correspond to the numbers one by one, but it is time-consuming and laborious. , high cost, low efficiency and other shortcomings are obvious. Now, the common method for scie...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/6806
CPCC12Q1/6806C12Q2527/125
Inventor 郭涛陈淳夏澳运黄翠红周丹华黄明刘永柱王慧陈志强
Owner SOUTH CHINA AGRI UNIV
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