A three-mode digital holographic microscope imaging system

Abstract

The invention discloses a three-mode digital holography microscopy imaging system. The three-mode digital holography microscopy imaging system comprises a He-Ne laser, an optical fiber beam splitter,a mercury lamp, a white light LED, a frosted glass plate, a first beam expanding collimating mirror, a second beam expanding collimation mirror, a third beam expanding collimating mirror, a first beamsplitting prism, a second beam splitting prism, a microscope objective lens, a tube lens, an excitation color filter, a color filter turntable, an absorption color filter and a camera, wherein the color filter turntable switches three different modes of hologram, fluorescence and a bright field. The three-mode digital holography microscopy imaging system skillfully utilizes a color filter to integrate the fluorescence microscopy mode, the bright field microscopy mode and the digital holography microscopic mode into a whole, and the system utilization rate is high; in different modes, a samplearea observed by the system is consistent, and synthesis of horizontal information and axial information is facilitated; and an obtained composite image can simultaneously contain bright field imageinformation, fluorescence image information and phase information of samples.

Description

technical field [0001] The invention belongs to optical measurement and imaging technology, in particular to a three-mode digital holographic microscopic imaging system. Background technique [0002] As a new type of coherent measurement and imaging technology, digital holography has its greatest advantage in that it can simultaneously and independently acquire quantitative amplitude information and phase information of objects, and when quantitative measurements are performed on almost transparent objects such as biological cells, the phase information is particularly important. important. The invention patent "Transmission-type digital holographic microscope imaging device based on telecentric optical structure" (application number: 201510631831.3) has greatly improved the imaging quality of the system, but due to the illumination method of coherent light, the lateral imaging effect of the system is improved. It is far inferior to the traditional microscope that uses inco...

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Problems solved by technology

The invention patent "Transmissive Digital Holographic Microscopic Imaging Device Based on Telecentric Optical Structure" (application number: 201510631831.3) has greatly improved the imaging quality of the system, but due to the illumination method of coherent light, the horizontal imaging effect of the system It is far inferior to the traditional microscope with incoherent light as the illumination method, so the general digital holographic microscope has low lateral imaging clarity and has been criticized by biomedical researchers

However, inverted fluorescence microscopes and digital holographic microscopes are not only expensive, but also quite bulky

At the same time, after obtaining the bright field and fluorescence information of the sample under the inverted fluorescence microscope, it is difficult to find a completely consistent sample area in the digital holography system, which brings difficulties to the synthesis of lateral intensity information and axial thickness information. And if the observations are made separately in the two systems, the timeliness of the work is very low

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