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Organic toxin sample vibration purification detection method in grain crops, and ultrasonic pipette thereof

A detection method and pipetting gun technology, applied in the field of ultrasonic pipetting guns, can solve the problems of affecting the purification yield, unfavorable rapid detection and field collection detection, and high cost, so as to eliminate the background of impurity interference, improve the speed of sample purification, and promote The effect of fully mixed adsorption

Inactive Publication Date: 2019-08-09
苏州贞成分析仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, ① the above method requires special grain processing equipment, which is costly and is not conducive to the environment of rapid detection and field collection and detection
②During the purification process, the sample slowly flows through the solid-phase column only by gravity and silica gel adsorption, which takes too long and is inconvenient to operate, and the plant material particles are easy to block the column, which affects the purification yield
It is difficult to avoid contact with the external environment, which is likely to cause secondary pollution and affect the accuracy of detection

Method used

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  • Organic toxin sample vibration purification detection method in grain crops, and ultrasonic pipette thereof
  • Organic toxin sample vibration purification detection method in grain crops, and ultrasonic pipette thereof
  • Organic toxin sample vibration purification detection method in grain crops, and ultrasonic pipette thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Application Case of Zearalenone in Corn Flour

[0070] Sample extraction:

[0071] ·Weigh 6g cornmeal sample, pulverize and pass through a 1mm sieve, add to a 50mL centrifuge tube, add 0.6g sodium chloride and 15mL extract (90% acetonitrile-water solution) and mix well.

[0072] · Ultrasonic extraction for 30 minutes, put into a centrifuge (8000r / min centrifugation for 20 minutes), and take the supernatant as the sample extraction solution.

[0073] ·Sample purification:

[0074] Step 1: Take out the tip of the pipette, install it on the electric pipette, insert the tip below the liquid surface of the liquid to be purified, press the suction button of the electric pipette, inhale 1ml of the liquid to be purified, and stay for 5s.

[0075] Step 2: Move the tip of the pipette out of the liquid surface, press the suction button of the electric pipette again, and inhale 1ml of air to fully mix the liquid with the filler, repeat this step three times, and inhale 4ml of air...

Embodiment 2

[0095] Application Cases of Organic Toxins in Wheat

[0096] Sample extraction:

[0097] Weigh 5g of wheat sample, crush it and pass it through a 1mm sieve, add it to a 50mL centrifuge tube, dilute to 25mL with the extract (acetonitrile), and vortex to mix;

[0098] · Ultrasonic extraction for 30 minutes, put into a centrifuge (4000r / min centrifugation for 10 minutes), and take the supernatant as the sample extraction solution.

[0099] ·Sample purification:

[0100] Step 1: Take out the tip of the pipette, install it on the electric pipette, insert the tip below the liquid surface of the liquid to be purified, press the suction button of the electric pipette, inhale 1ml of the liquid to be purified, and stay for 5s.

[0101] Step 2: Move the tip of the pipette out of the liquid surface, press the suction button of the electric pipette again, and inhale 1ml of air to fully mix the liquid with the filler, repeat this step three times, and inhale 4ml of air in total.

[0102]...

Embodiment 3

[0123] Application Case of Vomitin in Cornmeal

[0124] Sample extraction:

[0125] Weigh 5g of cornmeal sample, crush it and pass it through a 1mm sieve, add it to a 50mL centrifuge tube, dilute to 25mL with the extract (84wt%, acetonitrile / water), and vortex to mix;

[0126] · Ultrasonic extraction for 30 minutes, put into a centrifuge (8000r / min centrifugation for 20 minutes), and take the supernatant as the sample extraction solution.

[0127] ·Sample purification:

[0128] Step 1: Take out the tip of the pipette, install it on the electric pipette, insert the tip below the liquid surface of the liquid to be purified, press the suction button of the electric pipette, inhale 1ml of the liquid to be purified, and stay for 5s.

[0129] Step 2: Move the tip of the pipette out of the liquid surface, press the suction button of the electric pipette again, and inhale 1ml of air to fully mix the liquid with the filler, repeat this step three times, and inhale 4ml of air in total...

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Abstract

The invention provides an organic toxin sample vibration purification detection method in grain crops, and an ultrasonic pipette thereof, and belongs to the technical field of the chemical analysis and detection. The detection step comprises the following steps: extracting a sample, purifying the sample, vibrating and extracting, and draining liquid from the sample. The pipetting technology and the sample purification technology are combined, the purification process of a to-be-detected sample is accomplished when realizing the liquid transferring, the operation step is greatly saved, and thedetection precision is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a vibration purification detection method for organic toxin samples in cereal crops and an ultrasonic pipetting gun thereof. Background technique [0002] Food crops, especially cereal crops, need to go through a long storage and processing process from the initial artificial planting or mechanical harvesting, to drying and shelling, and finally storage. The storage of ordinary grains often does not add any antiseptic ingredients to the grain crops, and only relies on drying and dehydration for storage. Therefore, grain mildew often occurs due to improper processing and storage or excessive circulation time. [0003] Organic toxins in food are metabolized by various molds, such as zearalenone, deoxynivalenone, aflatoxin, etc., which are a variety of highly toxic carcinogens. A very important step in the pre-process of grain processing is the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 靳钊谢尚晋
Owner 苏州贞成分析仪器有限公司
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