Drug sensitivity test plate and method for quickly detecting drug-resistance phenotype

Abstract

The invention discloses a drug sensitivity test plate and method for quickly detecting a drug-resistance phenotype and is applied to quick detection for the drug-resistance phenotype. M-H agar with the thickness of 3 millimeters is evenly laid in a drug sensitivity test dish to form tMHA. The tMHA is coated with 2.0-3.0 Maxwell unit of a bacterial solution, a drug sensitivity test paper piece is attached to the tMHA, and just through culture for 4-6 hours, whether or not the corresponding drug-resistance phenotype exists can be judged according to the diameter of an antibacterial ring. According to the thin M-H drug sensitivity test plate, the time of detecting the drug-resistance phenotype of bacteria can be shortened to 4-8 hours from the shortest time of 16-24 hours in the prior art, quick, convenient and easy detection for the drug-resistance phenotype of the bacteria has extremely important clinical significance on timely and effective anti-infection treatment, and the plate is beneficial to clinical timely treatment and prevention and control over hospital infection.

Description

technical field [0001] The invention relates to the detection of bacterial drug-resistant phenotypes, in particular to a drug-sensitivity plate for rapid detection of drug-resistant phenotypes and a method for using the same. Background technique [0002] With the widespread use of antibiotics worldwide, bacterial resistance to antibiotics has also followed. There are many mechanisms of bacterial resistance to antibiotics, such as the production of hydrolytic enzymes and the loss of membrane permeable proteins. At present, bacterial drug resistance has become an important factor threatening human health, and infectious diseases rank first among the causes of global death every year. Drug-resistant strains such as carbapenem-resistant Enterobacteriaceae (CRE) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are widely spread around the world. In China, the CRE strains of Klebsiella pneumoniae have reached 9%, and even exceeded 20% in some areas, and ...

AI Technical Summary

Problems solved by technology

In these phenotypic detection methods, the bacteria to be tested are spread on the M-H agar plate, and different drug-sensitive paper sheets are affixed, and the drug-resistant phenotype is judged according to the size or shape of the inhibition zone, because on the M-H agar plate It generally takes 16-24 hours to form a bacteriostatic zone visible to the naked eye, so most of these detection methods take 16-24 hours, which is not conducive to timely clinical treatment and prevention and control of hospital infection

Although molecular biology and mass spectrometry methods can be quickly detected, these aspects have high requirements for equipment and technology, which is not conducive to the routine development of clinical laboratories.

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