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Anaerobic ammonium oxidation strain preservation and recovery method

An anammox bacteria and anammox technology are applied in the field of preservation and restoration of anammox strains, and can solve the biological rejection of anammox strains, anammox strain activity destruction, high equipment cost, complicated operation, etc. problems, to avoid biological rejection, restore denitrification performance, and achieve the effect of small footprint

Active Publication Date: 2019-08-16
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preservation effect of the cold storage method is poor, and the mortality rate of the bacteria is high; the equipment cost of the freezing method and the freeze-drying method are high and the operation is cumbersome, and it is not conducive to large-scale industrial application; the cryoprotectant is expensive and cannot be used on a large scale. May cause biorejection of anammox strains or cause irreversible damage to the activity of anammox strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The sludge containing the anammox strains to be preserved was washed 3 times with an inorganic salt solution, put into a 1L storage container made of organic plastic, and stored in units of 0.5L sludge. Then add 0.5L of protective agent into the storage container, the protective agent consists of 437.5mL of distilled water and 62.5mL of anammox bacteria EPS, and then argon gas aeration for 20min and place it at 4°C for sealed storage.

[0029] The storage container made of organic plastic is a clean and airtight plastic bottle. The activity of the anammox strain to be preserved is 138.72 mg N (g VSS d) -1 .

[0030] The anammox bacteria EPS of the present embodiment is extracted by steam method, and the specific steps are:

[0031] Take 0.5mL of sludge and wash it with pure water for 3 times, add 10mL of pure water, centrifuge at 3200rpm for 30min, discard the supernatant, add 25mL of 0.9% normal saline to the remaining sludge sediment, and dissolve at 0.8MPa 1. High...

Embodiment 2

[0038] The sludge containing the anammox strains to be preserved was washed 3 times with an inorganic salt solution, put into a 1L preservation container made of organic plastic, and preserved in units of 0.5L of sludge. Then add 0.5L protective agent to the storage container, the protective agent consists of 437.5mL distilled water and 62.5mL anammox bacteria EPS, then argon aerate for 20min and store at room temperature (15-25°C).

[0039] The storage container made of organic plastic is a clean and airtight plastic bottle. The activity of the anammox strain to be preserved is 138.72 mg N (g VSS d) -1 .

[0040] After three months of storage, the activity of the anammox strain was 71.22 mg N (g VSS d) -1 , the activity retention rate was 51.34%, indicating that this method can effectively preserve the anammox strains.

[0041] Then the sludge containing the anammox strains was taken out from the storage container, and washed 3 times with tap water and inorganic salt solut...

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Abstract

The invention discloses an anaerobic ammonium oxidation strain preservation method. The method includes: flushing sludge containing an anaerobic ammonium oxidation strain with inorganic salt solution,and keeping in a preservation container; adding a protecting agent into the preservation container, well mixing, removing dissolved oxygen in mixed solution, and preserving at 4-25 DEG C, wherein theprotecting agent is a mixture of water and an anaerobic ammonium oxidation bacterium extracellular polymeric substance. By adoption of the protecting agent which is a biological endogenous substance,biological exclusion is avoided, and guidance significance to anaerobic ammonium oxidation strain preservation at different temperature conditions can be achieved. The invention further discloses ananaerobic ammonium oxidation strain preservation recovery method. The recovery method includes that the anaerobic ammonium oxidation strain is taken out from the preservation container, flushed with water and the inorganic salt solution respectively and recovered in a reactor. By adoption of the methods, the anaerobic ammonium oxidation strain can be preserved effectively, and the denitrificationperformance of the preserved sludge can be recovered in a short time.

Description

technical field [0001] The invention relates to the technical field of preservation of anammox strains, in particular to a method for preservation and restoration of anammox strains. Background technique [0002] Nitrogen pollution in water has become one of the important issues threatening the ecosystem and human health, and effective control of nitrogen pollutants needs to be solved urgently. Anammox has great development value in the field of wastewater treatment due to its advantages of high efficiency, low energy consumption, and no secondary pollution. However, due to the extremely slow growth of anammox bacteria, the start-up period for this process to be applied to actual wastewater treatment is too long. The world's first industrialized anaerobic ammonium oxidation process to treat ammonia nitrogen wastewater took up to 3.5 years. [0003] Therefore, long-term storage of anammox bacteria, establishment of a strain bank, and provision of sufficient provenance for s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/20C02F3/28C02F3/34C02F101/16C12R1/01
CPCC02F3/2846C02F3/34C02F2101/16C02F2101/166C12N1/04C12N1/20
Inventor 范念斯白昱慧张权吴静倪绍凯金仁村
Owner HANGZHOU NORMAL UNIVERSITY
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