Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preservation and recovery method of anaerobic ammox strain

An anammox bacteria and anaerobic ammonium oxidation technology, applied in the field of preservation and recovery of anammox strains, can solve the problem of anammox strain biological rejection, anammox strain activity destruction, high equipment cost, cumbersome operation, etc. problems, achieve the effects of avoiding biological rejection, restoring denitrification performance, and occupying a small area

Active Publication Date: 2021-05-11
HANGZHOU NORMAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preservation effect of the cold storage method is poor, and the mortality rate of the bacteria is high; the equipment cost of the freezing method and the freeze-drying method are high and the operation is cumbersome, and it is not conducive to large-scale industrial application; the cryoprotectant is expensive and cannot be used on a large scale. May cause biorejection of anammox strains or cause irreversible damage to the activity of anammox strains

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The sludge containing the anammox strains to be preserved was washed 3 times with an inorganic salt solution, put into a 1L storage container made of organic plastic, and stored in units of 0.5L sludge. Then add 0.5L of protective agent to the storage container, the protective agent consists of 437.5mL of distilled water and 62.5mL of anammox bacteria EPS, and then argon gas aeration for 20min and place it at 4°C for sealed storage.

[0029] The storage container made of organic plastic is a clean and airtight plastic bottle. The activity of the anammox strain to be preserved is 138.72 mg N (g VSS d) -1 .

[0030] The anammox bacteria EPS of the present embodiment is extracted by steam method, and the specific steps are:

[0031] Take 0.5mL of sludge and wash it with pure water for 3 times, add 10mL of pure water, centrifuge at 3200rpm for 30min, discard the supernatant, add 25mL of 0.9% normal saline to the remaining sludge sediment, and dissolve at 0.8MPa 1. High-p...

Embodiment 2

[0038] The sludge containing the anammox strains to be preserved was washed 3 times with an inorganic salt solution, put into a 1L preservation container made of organic plastic, and preserved in units of 0.5L of sludge. Then add 0.5L protective agent to the storage container, the protective agent consists of 437.5mL distilled water and 62.5mL anammox bacteria EPS, then argon aerate for 20min and store at room temperature (15-25°C).

[0039] The storage container made of organic plastic is a clean and airtight plastic bottle. The activity of the anammox strain to be preserved is 138.72 mg N (g VSS d) -1 .

[0040] After three months of storage, the activity of the anammox strain was 71.22 mg N (g VSS d) -1 , the activity retention rate was 51.34%, indicating that this method can effectively preserve the anammox strains.

[0041] Then the sludge containing the anammox strains was taken out from the storage container, and washed 3 times with tap water and inorganic salt solut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preserving anammox strains, which comprises: washing the sludge containing anammox strains with an inorganic salt solution and storing them in a preservation container; adding a protective agent into the preservation container and mixing them evenly , remove the dissolved oxygen in the mixed solution and store it at 4-25°C; the protective agent is a mixture of water and extracellular polymer of anammox bacteria. The protective agent used in the invention is the endogenous substance of organisms, which avoids the rejection of organisms; and has certain guiding significance for the preservation of anammox strains under different temperature conditions. The invention also discloses a method for recovering the anammox strains. The anammox strains are taken out from the storage container, rinsed with water and the inorganic salt solution respectively, and placed in a reactor for recovery. The invention can effectively preserve the anaerobic ammonium oxidation bacteria species, and can restore the denitrification performance of the preserved sludge in a short time.

Description

technical field [0001] The invention relates to the technical field of preservation of anammox strains, in particular to a method for preservation and restoration of anammox strains. Background technique [0002] Nitrogen pollution in water has become one of the important issues threatening the ecosystem and human health, and effective control of nitrogen pollutants needs to be solved urgently. Anammox has great development value in the field of wastewater treatment due to its advantages of high efficiency, low energy consumption, and no secondary pollution. However, due to the extremely slow growth of anammox bacteria, the start-up period for this process to be applied to actual wastewater treatment is too long. The world's first industrialized anaerobic ammonium oxidation process to treat ammonia nitrogen wastewater took up to 3.5 years. [0003] Therefore, long-term storage of anammox bacteria, establishment of a strain bank, and provision of sufficient provenance for s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12N1/20C02F3/28C02F3/34C02F101/16C12R1/01
CPCC02F3/2846C02F3/34C02F2101/16C02F2101/166C12N1/04C12N1/20
Inventor 范念斯白昱慧张权吴静倪绍凯金仁村
Owner HANGZHOU NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com