Matrix for evaluating capability of producing hexanoic acid by pit mud and method for evaluating capability of producing hexanoic acid by pit mud
A technology for pit mud and caproic acid, which is applied in the field of winemaking, can solve the problems of no effective evaluation method for the capacity of pit mud to produce caproic acid, and achieve the effects of simple detection method and accurate result.
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Embodiment 1
[0068] This embodiment provides a matrix for evaluating the ability of pit mud to produce caproic acid, and a method for evaluating the ability of pit mud to produce caproic acid using the matrix.
[0069] The pH value of the pit mud to be evaluated was detected to be 6.2.
[0070] The matrix used to evaluate the ability of pit mud to produce caproic acid includes the following components in mass percentage:
[0071] 0.3% CH 3 COONa, 0.04% (NH 4 ) 2 SO 4 , 0.03% K 2 HPO 4 , 0.01% MgSO 4 , 0.2% yeast paste and 4% 95% vol alcohol, the rest of the water.
[0072] The pH of the matrix was adjusted to pH 6.2 with lactic acid.
[0073] The method for evaluating the ability of pit mud to produce hexanoic acid using the above matrix is as follows;
[0074] Take 3 150ml Erlenmeyer flasks, and put 144mL of substrate except alcohol into the Erlenmeyer flasks.
[0075] Autoclave the flask at 0.1Mpa for 30 minutes, cool to 32°C, take 1.5g of pit mud to be evaluated and add it t...
Embodiment 2
[0079] This embodiment provides a matrix for evaluating the ability of pit mud to produce caproic acid, and a method for evaluating the ability of pit mud to produce caproic acid using the matrix.
[0080] The pit mud to be evaluated in this embodiment is the same as the pit mud to be evaluated in Example 1.
[0081] The matrix used to evaluate the ability of pit mud to produce caproic acid includes the following components in mass percentage: 0.1% CH 3 COONa, 0.02% (NH 4 ) 2 SO 4 , 0.01% K 2 HPO 4 , 0.008% MgSO 4 , 0.1% yeast extract; 2% volume fraction is 92% ethanol aqueous solution, and the remainder is water.
[0082] The pH of the matrix was adjusted to pH 6.2 with lactic acid.
[0083] The method for evaluating the ability of pit mud to produce hexanoic acid using the above matrix is as follows;
[0084] Take 3 150ml Erlenmeyer flasks, and put 144mL of substrate except alcohol into the Erlenmeyer flasks.
[0085] Autoclave the flask at 0.12Mpa and 115°C for 3...
Embodiment 3
[0090] This embodiment provides a matrix for evaluating the ability of pit mud to produce caproic acid, and a method for evaluating the ability of pit mud to produce caproic acid using the matrix.
[0091] The pH value of the pit mud to be evaluated is 4.8.
[0092] The matrix used to evaluate the ability of pit mud to produce caproic acid includes the following components in mass percentage:
[0093] 0.3% CH 3COONa, 0.04% (NH 4 ) 2 SO 4 , 0.03% K 2 HPO 4 , 0.01% MgSO 4 , 0.2% yeast extract and 4% 95% vol alcohol, and the balance is water.
[0094] The pH of the matrix was adjusted to pH 4.8 with acetic acid.
[0095] The method for evaluating the ability of pit mud to produce hexanoic acid using the above matrix is as follows;
[0096] Take 3 150ml Erlenmeyer flasks, and put 144mL of hexanoic acid production capacity evaluation medium of pit mud except alcohol into the Erlenmeyer flasks;
[0097] Autoclave the flask at 0.1Mpa for 30 minutes, cool to 32°C, take 1.5...
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