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Method for identifying camellia japonica color bud mutation varieties

An identification method and color technology, applied in the biological field, can solve problems such as no related reports, and achieve the effects of high repeatability, economical application and strong stability

Active Publication Date: 2019-08-16
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, molecular marker technology is mainly used in the identification of bud mutation varieties, such as the application of ISSR or SSR in apples, pears and other fruit trees to identify their bud mutations, and the use of flower color and anthocyanin components and contents in petals to identify camellia color bud mutation varieties There are no related reports

Method used

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  • Method for identifying camellia japonica color bud mutation varieties
  • Method for identifying camellia japonica color bud mutation varieties
  • Method for identifying camellia japonica color bud mutation varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1 material

[0052] Fresh flowers of the camellia variety 'Red Furong' and its bud varieties 'Fen Furong' and 'White Furong' were collected to determine the flower color, among which the pink part of the petals was cut from 'Fen Furong', and the white part of the petals was cut from 'White Furong'. Flower color determination and qualitative and quantitative analysis. For each material, 5 plants with the same growth condition were selected, and 3 flowers in the blooming stage on the periphery of the crown were selected for each plant.

[0053] 2 color determination

[0054] Flower color determination was carried out according to the above method.

[0055]The flower color measurement data of camellia 'Hong Furong' and its bud varieties are shown in Table 2.

[0056] Table 2 Flower color data of camellia 'Hong Furong' and its bud varieties

[0057]

[0058] Note: a , b, c Indicates that the multiple comparison SNK test is significant at the P=0.05 significance lev...

Embodiment 2

[0069] 1 material

[0070] Collect the fresh flowers of the camellia variety 'Hongchang'ecai' and its bud varieties 'Fenchang'ecai' and 'White Chang'ecai' to determine its flower color, among which the pink part of the petals is cut from 'Fenchang'ecai', and the pink part of the petals is cut from 'White Chang'ecai' The white part of the petals was tested for flower color and qualitative and quantitative analysis. For each material, 5 plants with the same growth condition were selected, and 3 flowers in the blooming stage on the periphery of the crown were selected for each plant.

[0071] 2 color determination

[0072] Flower color determination was carried out according to the above method.

[0073] The flower color measurement data of camellia 'Hongchang'ecai' and its bud varieties are shown in Table 5, CIE L * a * b * In the system, from red 'Red Chang'e Color' to pink 'Pink Chang'e Color' and white 'White Chang'e Color', as the color of the petals becomes lighter, it...

Embodiment 3

[0084] 1 material

[0085] Collect fresh flowers of the camellia variety 'Red Wubao' and its bud varieties 'Fen Wubao' and 'White Wubao' to determine its flower color, among which the pink part of the petals is cut from 'Fen Wubao', and the pink part of the petals is cut from 'White Wubao'. The white part of the petals was tested for flower color and qualitative and quantitative analysis. For each material, 5 plants with the same growth condition were selected, and 3 flowers in the blooming stage on the periphery of the crown were selected for each plant.

[0086] 2 color determination

[0087] Flower color determination was carried out according to the above method.

[0088] The flower color test data of camellia 'Hongwubao' and its bud varieties are shown in Table 8, CIE L * a * b * In the system, from red 'Hong Wubao' to pink 'Fen Wubao' and white 'White Wubao', as the color of the petals became lighter, the L* value gradually increased, and 'White Wubao' was significa...

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Abstract

The invention discloses a method for identifying camellia japonica color bud mutation varieties. A high performance liquid chromatography-photodiode array detection and ultra-high performance liquid chromatography-quadrupole rod-gas chromatography-time-of-flight mass spectrometry is used for researching the composition and content of anthocyanins in red petals of the camellia japonica variety andpetals of pink and white bud mutation varieties, combined with the phenotypic analysis of flower colors, the camellia japonica color bud mutation is effectively identified, the relationship between the camellia japonica color bud mutation and the anthocyanins is determined, and the material base of forming the camellia japonica color bud mutation is revealed, so that a scientific basis is providedfor the breeding of the camellia japonica color bud mutation.

Description

technical field [0001] The invention relates to a method for identifying color bud change of camellias by utilizing flower color and anthocyanin components and contents in petals, and belongs to the field of biotechnology. Background technique [0002] Camellia japonica blooms in winter and spring. It has high ornamental value and strong adaptability. It is an important woody flower for landscaping and potted planting, and is widely used at home and abroad. Camellia has rich color variations, its original flower color is red, and it can produce pink, white and double-colored varieties through bud transformation. Corresponding pink and white varieties were selected from the flower varieties. Plant anthocyanins mainly include flavonoids, carotenoids and alkaloids. The anthocyanins in Camellia plants are mainly flavonoids, including anthocyanins in the red petals of Camellia japonica and flavonoids in the yellow petals of Camellia japonica (C.nitidissima) Alcohols, etc. Stud...

Claims

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Application Information

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IPC IPC(8): G01N30/02A01G7/00
CPCG01N30/02A01G7/00
Inventor 李辛雷王洁殷恒福范正琪李纪元
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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