Method for preparing high-purity phloretin
A high-purity technology for phloretin, applied in the field of separation and purification, can solve the problems of low purity and color of phloretin, and achieve the effect of environmental protection and optimization of extraction methods
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Embodiment 1
[0018] S1: Take 50g of phloretin raw material to be purified, the purity of the phloretin raw material is the first predetermined value, the first predetermined value is less than 85%, in this example 83% (HPLC detection purity, detection method using GB1886. 261-2016 method);
[0019] S2. Dissolve the phloretin raw material in an ethanol aqueous solution with a concentration of 60%, then add pure water with the same temperature and volume as the ethanol aqueous solution, use the solution as the sample solution, and pass the D101 macroporous resin column chromatography ( The column volume is 3L, the column diameter is 8cm, and the column height is 1.2m) for enrichment and purification. The weight of the ethanol aqueous solution added is 4-6 times the weight of the phloretin raw material. Specifically, in this example, ethanol The volume of both the aqueous solution and pure water is 250ml, and the temperature is 60℃;
[0020] S3. The chromatographic column in S2 is eluted with a 3...
Embodiment 2
[0025] S1: Take 60g of phloretin raw material to be purified, the purity of phloretin raw material is the first predetermined value, the first predetermined value is less than 85%, in this example 83% (HPLC detection purity, detection method using GB1886. 261-2016 method);
[0026] S2. Dissolve the phloretin raw material in a 65% ethanol aqueous solution, then add pure water with the same temperature and volume as the ethanol aqueous solution, use the solution as the sample solution, and pass it through the AB-8 model macroporous resin column layer (Column volume is 3L, column diameter is 8cm, column height is 1.2m) for enrichment and purification, and the weight of the ethanol aqueous solution added is 4-6 times the weight of the phloretin raw material. Specifically, this example In, the volume of ethanol aqueous solution and pure water are both 300ml, and the temperature is 55℃;
[0027] S3. The chromatographic column in S2 is eluted with 5% ethanol aqueous solution, the elution...
Embodiment 3
[0032] S1: Take 40g of phloretin raw material to be purified, the purity of phloretin raw material is the first predetermined value, the first predetermined value is less than 85%, in this example 83% (HPLC detection purity, detection method using GB1886. 261-2016 method);
[0033] S2. Dissolve the phloretin raw material in a 62% ethanol aqueous solution, then add pure water with the same temperature and volume as the ethanol aqueous solution, use the solution as the sample solution, and pass it through the HP-20 model macroporous resin column layer (Column volume is 3L, column diameter is 8cm, column height is 1.2m) for enrichment and purification, and the weight of the ethanol aqueous solution added is 4-6 times the weight of the phloretin raw material. Specifically, this example In, the volume of ethanol aqueous solution and pure water are both 200ml, and the temperature is 50℃;
[0034] S3. The chromatographic column in S2 is eluted with a 7% ethanol aqueous solution, the elut...
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