Method for preparing high-purity phloretin

A high-purity technology for phloretin, applied in the field of separation and purification, can solve the problems of low purity and color of phloretin, and achieve the effect of environmental protection and optimization of extraction methods

Inactive Publication Date: 2019-08-23
SUZHOU HEYAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of high-purity phloretin, which solves the problems of low purity and color of existing phloretin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] S1: Take 50g of phloretin raw material to be purified, the purity of the phloretin raw material is the first predetermined value, the first predetermined value is less than 85%, in this example 83% (HPLC detection purity, detection method using GB1886. 261-2016 method);

[0019] S2. Dissolve the phloretin raw material in an ethanol aqueous solution with a concentration of 60%, then add pure water with the same temperature and volume as the ethanol aqueous solution, use the solution as the sample solution, and pass the D101 macroporous resin column chromatography ( The column volume is 3L, the column diameter is 8cm, and the column height is 1.2m) for enrichment and purification. The weight of the ethanol aqueous solution added is 4-6 times the weight of the phloretin raw material. Specifically, in this example, ethanol The volume of both the aqueous solution and pure water is 250ml, and the temperature is 60℃;

[0020] S3. The chromatographic column in S2 is eluted with a 3...

Embodiment 2

[0025] S1: Take 60g of phloretin raw material to be purified, the purity of phloretin raw material is the first predetermined value, the first predetermined value is less than 85%, in this example 83% (HPLC detection purity, detection method using GB1886. 261-2016 method);

[0026] S2. Dissolve the phloretin raw material in a 65% ethanol aqueous solution, then add pure water with the same temperature and volume as the ethanol aqueous solution, use the solution as the sample solution, and pass it through the AB-8 model macroporous resin column layer (Column volume is 3L, column diameter is 8cm, column height is 1.2m) for enrichment and purification, and the weight of the ethanol aqueous solution added is 4-6 times the weight of the phloretin raw material. Specifically, this example In, the volume of ethanol aqueous solution and pure water are both 300ml, and the temperature is 55℃;

[0027] S3. The chromatographic column in S2 is eluted with 5% ethanol aqueous solution, the elution...

Embodiment 3

[0032] S1: Take 40g of phloretin raw material to be purified, the purity of phloretin raw material is the first predetermined value, the first predetermined value is less than 85%, in this example 83% (HPLC detection purity, detection method using GB1886. 261-2016 method);

[0033] S2. Dissolve the phloretin raw material in a 62% ethanol aqueous solution, then add pure water with the same temperature and volume as the ethanol aqueous solution, use the solution as the sample solution, and pass it through the HP-20 model macroporous resin column layer (Column volume is 3L, column diameter is 8cm, column height is 1.2m) for enrichment and purification, and the weight of the ethanol aqueous solution added is 4-6 times the weight of the phloretin raw material. Specifically, this example In, the volume of ethanol aqueous solution and pure water are both 200ml, and the temperature is 50℃;

[0034] S3. The chromatographic column in S2 is eluted with a 7% ethanol aqueous solution, the elut...

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Abstract

The present invention relates to a method for preparing high-purity phloretin. The method comprises the following steps: S1, taking a phloretin raw material to be purified, wherein the purity of the phloretin raw material is a first pre-value; S2, dissolving the phloretin raw material in an ethanol-water solution having a concentration of 60-70%, adding pure water having the same temperature and volume to the ethanol-water solution, injecting the obtained solution as a sample solution, and carrying out chromatography column enrichment and purification; S3, sequentially eluting the chromatography column in step 2 with an ethanol-water solution having a concentration of 3-7% and an ethanol-water solution having a concentration of 62-67%; and S4, collecting an eluate obtained by the elution of the chromatography column with the ethanol-water solution having the concentration of 62-67%, carrying out reduced pressure concentration until the eluate has no alcohol smell, cooling the obtainedconcentrate to room temperature, centrifuging the cooled eluate, and recovering the obtained solid to obtain the purified phloretin, wherein the purity of the purified phloretin is higher than the first pre-value. The ethanol-water solution with the concentration of 62-67% is used to dissolve the phloretin in order to extract phloretin with high purity and no mixed colors, and the use range is notlimited; and the method has the advantages of simplicity, easiness in use, and environmental protection, and the purity of the obtained phlorizin is equal to or more than 98%.

Description

Technical field [0001] The invention relates to the technical field of separation and purification, and more specifically, it relates to a method for preparing high-purity phloretin. Background technique [0002] Phloretin, alias trihydroxyphenol acetone 2,4,6-trihydroxy-3-(4-hydroxyphenyl)propiophenone. It is a new type of natural skin whitening agent recently researched and developed abroad, mainly distributed in the peel and root bark of juicy fruits such as apples and pears. Phhroretin can be used in facial masks, skin creams, lotions and essences. It is a pearl white crystalline powder, soluble in ethanol and acetone, but almost insoluble in water. In addition, it has multiple biological activities such as anti-oxidation, inhibition of tyrosinase activity, anti-cancer, anti-inflammatory, antibacterial and estrogen effects. In recent years, phloretin has been widely used in the food field as an antioxidant function factor. [0003] However, the content of phloretin in plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C45/78C07C49/83
CPCC07C45/78C07C49/83
Inventor 曾鸣
Owner SUZHOU HEYAN BIOTECH
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